Circadian clock-coordinated hepatic lipid metabolism: only transcriptional regulation?

By regulating the metabolism of fatty acids, carbohydrates, and xenobiotic, the mammalian circadian clock plays a fundamental role on the liver physiology. At present, it is supposed that the circadian clock regulates metabolism mostly by regulating the expression of liver enzymes at the transcriptional level. However, recent evidences suggest that some signaling pathways synchronized by the circadian clock can also influence metabolism at a post-transcriptional level. In this context, we have recently shown that the circadian clock synchronizes the rhythmic activation of the IRE1alpha pathway in the endoplasmic reticulum. The absence of circadian clock perturbs this secondary clock, provokes deregulation of endoplasmic reticulum-localized enzymes, and leads to impaired lipid metabolism. We will describe here the additional pathways synchronized by the clock and discussed the influence of the circadian clock-controlled feeding rhythm on them.

complex luminal network in which protein synthesis, maturation, folding, and transport take place. It has been previously shown that the ER of hepatocytes exhibits a circadian dilatation which is a sign of ER stress [8]. This ER stress triggers the unfolded protein response (UPR) which is a conserved adaptative response to cope with the accumulation of unfolded proteins in this organelle. When unfolded proteins accumulate in ER, three pathways are activated, IRE1α, PERK and ATF6, which lead to the nuclear translocation of the transcription factors XBP1, ATF4 and ATF6, respectively. These transcription factors activate in turn the expression of genes coding for proteins involved in peptide folding and degradation to limit the accumulation of unfolded proteins [9]. In this context, we have recently described the posttranslational regulation of liver enzymes through a circadian clockcoordinated 12-hours period rhythmic activation of the IRE1α pathway [10]. The observed rhythmic activation of the IRE1α pathway leads to the expression with a 12hours period of the XBP1-regulated genes that are included in the 12-hours period genes described recently in mouse liver [11]. Persistent activation of the IRE1α pathway in circadian clock deficient Cry1/Cry2 ko mice induced the downregulation of ER membrane localized enzymes, including HMGCR and SCD1, leading to a perturbed lipid metabolism in the liver of this mice. The decreased expression of these enzymes could be caused by activation of the ER Associated Degradation (ERAD), a process involved in the elimination of unfolded proteins inside the ER [12] regulated by the IRE1α-XBP1 pathway [13], which has been shown to induce the degradation of HMGCR and SCD1. In addition, IRE1α is a ribonuclease that can also induce endonucleolytic decay of many ERlocalized mRNA including Hmgcr mRNA [14,15]. These two functions could contribute in parallel to the regulation of lipid metabolism by ER stress. Elsewhere, the IRE1α-XBP1 pathway controls also lipid metabolism through direct transcriptional regulation of the genes Scd1, Dgat2 and Acc2 involved in lipogenesis. As a consequence, liver-specific deletion of the Xpb1 gene resulted in a dramatic reduction of plasma lipids [16]. Finally, it has been shown that ER stress induces the degradation of the apolipoprotein ApoB100 [17,18] and then blocks VLDL secretion [19], which might be responsible for the fat accumulation in the liver in tunicamycin-injected mice [20].
Interestingly, IRE1α activation has been recently linked to induction of autophagy through activation of the Jun-Kinase pathway [21]. In addition, a genomic screen in fly cells demonstrated that knocking down genes involved in protein folding inside the ER or in the UPR, including Xbp1, increases basal autophagy levels [22]. Autophagy is a survival pathway classically associated with adaptation to nutrient starvation [23] and, as UPR, autophagy presented a diurnal rhythm of activation in rodent liver [24,25]. This is of particular interest if we consider the fact that autophagy is linked to lipid metabolism through regulation of intracellular lipid stores [26]. As a consequence, mice with an adipose tissue-specific deletion of the Atg7 gene, an important regulator of autophagy, present an important defect in lipid storage [27,28]. IRE1α-dependent rhythmic regulation of autophagy could then participates to the circadian clock-coordinated lipid metabolism in mammals.
The disturbed metabolism observed in Cry1/Cry2 ko mice is probably responsible of the aberrant activation of the Sterol Responsive Element Binding Protein (SREBP) transcription factor, an ER membrane bond protein that, in low sterol conditions, translocates to the Golgi to be cleaved and released in order to migrate in the nucleus where it activates genes coding for enzymes involved in cholesterol and fatty acid metabolism [29]. It has been shown that the ER stress induced activation of SREBP1 and SREBP2 [30,31] correlates with the depletion of INSIG regulatory proteins probably through a decreased synthesis of the protein [32,33]. Interestingly, the circadian clock influences also the activation of the SREBP pathway through the control of Insig2 mRNA expression [34]. Both transcriptional and post-transcriptional circadian clock-coordinated events seem to be involved in the rhythmic activation of the SREBP pathway.
As summarised in Figure 1, in addition to their rhythmic activation, all these pathways have in common the fact that they are regulated by feeding-fasting events. However, this feeding rhythm, like most behaviour, is also controlled by the circadian clock. To discriminate the genes dependant or not on a functional local circadian oscillator, this local clock has been inactivated in mouse liver. This strategy reveals that the expression of approximately 90 % of the rhythmic genes is dependent on a functional circadian clock and only 10 % is dependent on systemic cues [35]. However, the influence of feeding on rhythmic gene expression has been evaluated by a recent study which discriminates between gene induced by feeding and fasting. As expected, food-induced and food-repressed genes present a rhythmic expression which is shifted in response to a change in the feeding schedule [36]. More interestingly, this shift in the feeding schedule is able to induce rhythmic expression of food-regulated genes in the liver of Cry1/Cry2 ko mice. These two studies raise www.impactaging.com the question of the differential influence of the molecular circadian oscillator and systemic cues on rhythmic gene expression: if these two signals can independently drive rhythmic gene expression, the circadian clock is able to fine-tune and modify feeding cues [34,36], whereas feeding cues can synchronize the molecular oscillator in peripheral organs [37].
However, feeding and food-regulated signals, as for example food regulated hormones like insulin, glucagon or leptin, did not represent the only circadian clockregulated cues that can influence lipid metabolism. For example, the pituitary-secreted growth hormone (GH) has been shown to influence lipid metabolism in mouse liver. Long term excess GH secretion produces high serum triglyceride levels through stimulated lipolysis [38], whereas inhibition of GH signaling induced perturbed lipid metabolism resulting in liver steatosis [39], probably caused by reduced activation of HNF3β [40]. Moreover, the ultradian secretion patterns of GH are directly responsible for the sexually dimorphic expression of several hepatic enzymes involved in steroids and fatty acids metabolism [41]. Interestingly, this dimorphism is impaired in Cry1/Cry2 ko mice, with males exhibiting a feminized liver likely because of altered ultradian GH secretion in absence of a functional circadian clock [42]. www.impactaging.com During aging, the circadian system becomes much less responsive to entrainment by light [43,44], and displays loss of temporal precision and robustness [45][46][47]. Such alterations of the circadian clock likely drive attenuation of the diurnal rhythm in circulating leptin [48]. Pulsatile GH secretion is also dramatically impaired in elderly subjects [49][50][51], leading to modifications in GHdependent liver metabolism that resemble those observed in clock-deficient animals [42,52]. Interestingly, the various UPR pathways also decline in the liver during aging [53], as well as autophagy [54]. In summary, many aspects of lipid metabolism that are regulated by the circadian clock exhibit profound changes when age increases, although the liver circadian oscillator appears preserved in aged rats [55]. These changes could thus at least partly originate from alterations of the network constituted of the central clock and other peripheral oscillators. In this respect, it is worth noting that mice bearing mutated alleles of the circadian genes Clock and Bmal1 display signs of premature aging [56,57]. The complexity of systemic cues influencing rhythmic gene expression has thus been rising during the last decade and defining the influence of these different signals on rhythmic gene expression will be thus an exciting challenge for the following years.