INFLUENCE OF L- LYSINE SULFATE ON CONTAINING OF VITAMINS AND MINERALS IN THE BODY OF BROILER CHICKENS

Experimental researches have shownthatthe L-lysine sulfate (the product of microbiological synthesis) has a positive effect on metabolism of vitamins A, E, C in the liver and minerals calcium and phosphorus in the blood and bones of broiler chickens. The dose of L-lysine sulfate of 800 mg∙kg 1 and 1000 mg∙kg -1 weight body has influence on increasing vitamins level in the liver. Was observed, that indexes of calcium and phosphorus are increased in the blood but it’s decreased in the bones of broiler chickens. This results are necessary for Veterinary Pharmacology Consults and official practically using in agricultural sectors of Russian Federation.

Introduction.L-Lysine Sulfate -feed additive used for enrichment and balance the rations o f agricultural animals.Along with lysine in a preparation there are other substances that enhance its nutritional value: carbohydrates, mineral salts, organic acids, more than 10% of other amino acids [1l .L-Lysine Sulfate has greater exchange energy than lysine in hydrochloride form.The use o f L-Lysine Sulfate in mixed feed production is economically viable and environmentally sound, and increase economic efficiency o f production [3l .
Vitamins and minerals play important role in the metabolism, growth and health in the body o f animals.Vitamin A has multiple functions: it is important for growth and development, for the maintenance o f the immune system and good vision [3l .Vitamin A is needed by the retina of the eye in the form o f retinal, which combines with protein opsin to form rhodopsin Minerals and vitamins are usually transported from the serosal side o f the mucosa to the liver in free or bound forms via the portal blood stream, but they can get 'stuck' in the mucosa.From the liver, they are Metabolism o f calcium and phosphorus binds with processes o f protein synthesis in the body as increasing mass muscles.Some experiments show, that chickens are able to adapt to early dietary changes in P and Ca through improvement o f digestive efficiency in a later phase, and the extent o f the compensation in terms o f growth performance and bone mineralization depends on the P and Ca levels in the subsequent diet [12! .
Thus, amino acid diet can influence on changes concentration minerals and vitamins in different organs and tissues in the organism broiler chickens.
The aim o f this study was evaluate the capacity concentrations vitamins A,E,C in the liver and P and Ca in the blood and bones o f broiler chickens with using diet from L-lysine sulfate.
M ethods a n d m aterials of research.
The experiments were on the broiler chickens of cross "Hubbard" in period age since 1 before 39 days.All animals was separated on five groups by 10 individuals in each.Birds of control and experimental groups had diet from main ration (MR) with balanced nutritionally and biologically active substances feed.Chickens experimental groups along with the basic diet received a daily dose supplements o f lysine sulfate in accordance with Table .1. Daily deliveryand delivery o f feed to chickens were carried out according to the recommendations o f the manufacturer o f cross-country "Hubbard".A t the end of the experience produced poultry slaughtered by decapitation and took tissue samples and organs for laboratory testing.Vitam ins A and E were determ ined with m ethod of spectral analysis; vitamin C -titrim etric m ethod with using solution of 2,6-dichlorophenol.
Calcium was research with trilonom etric m ethod in the bones and titrim etric by de W aard in the blood.Phosphorus was determined with method o f colorim etric by vanadate-m olybdenum reagent in the bones and in the blood.
The resulting m aterial was digitally processed using m athem atical m ethods of m athematical statistics, taken in Biology and M edicine (M icrosoft Excel 2007 application).
R esults an d discussion.
Indexes of the vitamins A, E, C was found in the liver o f broiler chickens.Results of testing are shown in the table 2. The heightened level o f vitamin A was in the liver o f animals which take diet from M R and lysine sulfate in dose of lysine sulfate 800 and 1000 mg-kg_1w eight body.Concentration o f vitam in С is increased with dose o f lysine sulfate more than 800 mg-kg_1w eight body.Index o f vitam in Е was reliably higher in third group in contrast with control.
As far as, ration of chickens did not contain other sources o f vitamins, that we extrapolate positive effect entering lysine sulfate in nutrition of broiler chickens.
Calcium and phosphorus are accum ulated in the bones and it takes part in functioning o f different biosynthesis process in the blood [9] .Therefore we described to check levels o f these microelem ents in the blood and in the bones.
Indexes o f calcium and phosphorus have trend to increaseconcentration o f this microelements in accordance with dose o f lysine sulfate in the blood of birds (fig.2).Change of concentrations calcium and phosphorus have trend to decrease this indexes in the bones of animals (fig.3).This aspect demonstrates as calcium and phosphorus increases in the blood in the reason of its extraction from the bones for future of protein synthesis.

Conclusions.
Using o f lysine sulfate in daily ration broiler chickens make possible increase vitamin A on 17% in the liver in dose 800 mg-kg-1 and 1000 mg-kg-1 weight body.Concentration o f vitamin E can increased before 30% with using lysine in diet.The level o f vitamin C also was heightened in dose 800 mg-kg-1weight body.Economic profitably use L-lysine sulfate in dose 800 mg-kg-1weight body.
Trend indexes o f calcium and phosphorus are increased in the blood but it's decreased in the bones o f broiler chickens with entering L-lysine sulfate.Calcium/phosphorus ratio is demonstrated in physiologically norm.

Figure 1 .
Figure 1.An illustration of ways flows of mineral between pools calcium and phosphorus in animal body (Braithwaite, 1983) [10!.

IFigure 2 .
Figure 2. Change indexes o f calcium and phosphorus in accordance with dose o f lysine sulfate in the blood o f the broiler chickens.RESEARCH RESULT: PHARMACOLOGY AND CLINICAL PHARMACOLOGY
Light, tem perature and other climate param eters comply with the established veterinary standards and norms[13].Tem perature, lighting and ventilationw as held in the room where the birds were kept.A soft bed o f fresh dry sawdust was changed every five days or added as needed.Daily weighing o f feed was conducted in accordance with the biological bird demand for nutrients.Live w eight o f chickens was m easured to an accuracy o f 1 g to morning feeding every five days during the period o f experience.RESEARCH RESULT: PHARMACOLOGY AND CLINICAL PHARMACOLOGY

Table 2 .
The quantity of vitamins in the liver (M±m).
* reliably difference between control and experimental index, р<0,05 Change indexes of calcium and phosphorus in accordance with dose of lysine sulfate in the bones of the broiler chickens.