A study of aerobic bacterial profile and their antibiogram in patients with chronic osteomyelitis with special references to staphylococcus aureus

Introduction: Chronic osteomyelitis is a important medical illness among developing countries, it is a very expensive disease for the patient and society mostly associated with trauma and surgery as risk factors. There is a constant change in the trend of organisms involved and resistance pattern seen with advent of newer antibiotics, So early and specific therapy is needed. With this background present study aims to look for the varying trends of microorganisms involved in osteomyelitis and their antimicrobial susceptibility pattern. Materials and Methods: A total of 100 cases studied over a period of one year, samples processed by following standard laboratory protocols and along with routine antimicrobial testing screening for MRSA done. Results: Among 100 cases, 76 were males and 24 were females, between age group of 11 –60 years. Long bones are most involved with trauma (45%) as risk factor. Staphylococcus aureus (51%) predominant pathogen isolated with 27(53%) were MRSA, followed by Pseudomonas aeruginosa (16%). Antibiotic sensitivity testing of gram positive organisms showed hundred percent sensitivity to Linezolid and Vancomycin with poor sensitivity to Pencillin (10/15.6%). Among gram negative organisms, majority showed highest sensitivity to Amikacin (73.3%) & Imipenem (71.1%) with poor sensitivity to Ciprofloxacin (20.0%) and Ampicillin (0%). Conclusion: Chronic osteomyelitis is a prime challenging problem in many countries with severe morbidity. Mostly due to neglect, delayed or inadequate treatment and emerging drug resistant pathogens involved. Culture based antibiotic therapy helps in effectively treating the disease also prevents the drug


Introduction
Chronic osteomyelitis is a persistent and relapsing disease, characterized by progressive destruction of bone and formation of sequestra. 1 The cavities formed will act as locus for persistent infection in the bone showing the chronic nature of the disease. Most of the cases factors contributing to chronic osteomyelitis is due to trauma with compound fracture, crush injury, surgical procedures, diabetic foot and sickle cell haemoglobinopathies. 2 This condition may be unifocal limited to single region of bone or multifocal may involve various regions of bone The commonest causative agents of Chronic osteomyelitis are Staphylococcus aureus, Coagulase negative staphylococci, Enterococci among gram positive organisms followed by Pseudomonas, Enterobacteriaceae spp and rarely Salmonella spp.and Actinomycetes. Among anaerobic organisms Peptostreptococcus spp., Bacteroides spp., Clostridium spp were involved. 4 Staphylococcus aureus constitutes 50%-75% cases of chronic osteomyelitis. In most of the cases infection is mono microbial, infection with multiple organisms are usually seen in diabetes mellitus patients with ulcer in foot. 5 The adhesion to local tissue is the initial event to takes place by Staphylococcus aureus strains which possess receptors for collagen, fibrinogen, fibronectin, bone sialoprotein, and heparin sulphate. 6 Infection is predisposed by trauma or injury thus exposing the binding sites for the organism. For staphylococcus aureus, the polysacch aride pseudocapsule also acts as an important virulence factor forming a strong bond between cell also involved with biofilm formation. Thus microcolonies are formed by the organism will be attached to each other and the adjacent tissue in the biofilm. The highly virulence factor glycocalayx also acts to protect the pathogen from host protective mechanism and antibiotics by forming mechanical barrier. 7 Thus glycocalyx prevents phagocytosis by forming the teichoic acid moiety and opsonization and also by altering the configuration of complement. Protein A an important virulence factor of staphylococcus aureus cell wall acts by interfering with opsonization and by polymorpho nuclear cells thus activating complement and inducing hypersensitivity reactions. Gram negative organisms are also evolving as emerging pathogens in chronic osteomyelitis cases, mostly associated with injury or spread from adjacent foci of infection and due to prolonged duration of stay in hospital.
Chronic osteomyelitis is a complicated disease due to its diverse nature of pathophysiology, clinical manifestations and management involved. Pathogenesis mainly involves the necrosis of bone, with sourrounding granulation tissue, followed by absorption of necrotic bone, with chronic reactive new bone and cicatrix formation with adjacent soft tissue destruction. The disease is characterized by progressive destruction of bone and the sequestration of bone. 8 Clinical features of chronic osteomyelitis vary according to severity of the bone involved, mostly presents with persistent pain and chronic discharging sinuses. Diagnosis of osteomyelitis based on clinical features, x-ray findings include bone lucency mixed with patchy sclerosis and adjacent new bone formation. MRI and confirmation of diagnosis by blood culture or culture of bone aspirate or biopsy.
Radiodiagnosis by CT scan and MRI are less often used to diagnose osteomyelitis, but they are frequently used to know the extent of disease involved and to know whether these are collections of pus that are amenable to drainage. The 'gold standard' specimen for diagnosing osteomyelitis is bone biopsy specimen. 9 In most of the cases antimicrobial therapy is based on culture obtained from deep bone biopsy sites or debrided tissues collected during surgery and their antimicrobial susceptibility pattern seen. Radio nucleotide scanning also helps in early diagnosis of osteomyelitis.
In children the predominant pathogen isolated is staphylococcus aureus contributing more than 90%. In case of adults, staphylococcus aureus commonly isolated which contributes around 50% to 75%. 10 While antimicrobial therapy is desirable in the control of osteomyelitis, surgery remains the therapeutic and diagnostic procedure. 11 In most of the cases staphylococcus aureus is the dominant pathogen isolated. Vancomycin is used as the prime drug for strains that are resistant to ampicillin and methicillin both. Linezolid a recently evolved drug which acts against MRSA cases due to its better oral bioavailability and good bone penetration seen. But the disadvantage is on prolonged therapy with linezolid is development of significant pancytopenia, peripheral neuropathy, optic neuritis. 12 Daptomycin a recently developed drug which has better bactericidal activity. Its usage in the vancomycin resistant Enterococcus cases has yet to be defined. The duration of therapy with antibiotics is around 4 to 6 weeks period. 13 The most common surgical intervention done for chronic osteomyelitis is sequestrectomy which includes removal of the diseased bone and adjacent soft tissue.
The main idea behind surgery is to completely remove every bit of an infection and thus maintaining a viable and vascular environment. Hyperbaric oxygen therapy can also been used frequently as an additional therapy for chronic osteomyelitis. The most encountered sequelae of persistent chronic osteomyelitis are lengthening of bone, pathologic fracture, contracture of muscles, epithelioma, reduced growth rate, scar tissue carcinoma and seconadary amyloidosis.
Difficulty arises to correctly establish the etiological agent and proper treatment to cure the patient. Proper selection of treatment always be made on basis of accurate diagnosis of the causative organisms and knowledge of pattern of susceptibility. Our study is done to emphasize the prime need of culture based antibiotic therapy and thus helps the clinicians in choosing appropriate antibiotics. Proper antibiotic coverage will also help in preventing emergence of resistance to the drug which are still sensitive.

Materials and Methods
The present study was a laboratory investigation based study, Study was done after getting permission from the institutional scientific and ethical committee approval, informed consent was obtained. Study was done for a period of one year, from Oct 2016 to Nov 2017, clinically and radiologically diagnosed cases of chronic osteomyelitis were included in this study. Cases apart from chronic osteomyelitis and those who underwent surgery with prosthetic joints were not included in the study. All the necessary data was collected from patient case sheets.
Under stringent aseptic measures followed, all samplespus, pus swabs and sequestrum were collected from cases with chronic osteo myelitis from deep wound site and sinus tract, for those cases who were attending the outpatient and inpatient department of orthopaedics, then they were subjected to bacteriological study over a period of 1 year. Two pus swabs were collected from cases subjected todirect gram stain and other for aerobic bacterial culture. Then the smear is stained by Gram's staining and viewed under microscope to look for the presence of pus cells and any microorganisms. The specimen is plated on Nutrient agar, Mac Conkey agar and Blood agar and incubated for 18 hrs at 37 • C. Culture isolates thus identified were further processed by observing the colony characteristics, culture smear and preliminary tests were done along with the controls by following a series of standard laboratory methods. After identification antibiogram was performed by lawn culture of organism on Muller Hinton agar plate by following standard Kirby-Bauer disc diffusion method and breakpoints interpretated according to CLSI guidelines. 13 Along with routine sensitivity testing MRSA detection was done by placing 30µg cefoxitin disc and interpretation was done by following CLSI guidelines. Clinical isolates showing zone diameters more than or equal to 22mm considered as susceptible to oxacillin (MSSA), minimal inhibitory concentration of vancomycin was performed by macro broth dilution method.

Results
Study group comprised, hundred chronic osteomyelitis cases.
Among hundred cases studied, Males were commonly affected compared to females, 76 males > 24 females. Majority of the cases were in the age group of 11-60 years. Positive cultures were 90% and negative culture 10%. Femur is the commonest bone involved in chronic osteomyelitis followed by Tibia, refer (Table 2). There was no statistical significance found between male and female with respect to site of bone involved p=0.709. The predominant risk factor found among 100 cases of chronic osteomyelitis, compound fracture due to trauma (45%), followed by postoperative infections 22(22%), 19 patients had diabetes with vascular insufficiency as predisposing factor, 10 patients(10%) had smoking and alcohol as predisposing factor and 4(4%) patients acquired infection following hematogenous spread. (Figure 1).

Discussion
Chronic osteomyelitis is an incessant and recurring disease difficult to treat and eradicate completely. In the absence of early diagnosis, prompt treatment or failure to antibiotic therapy leads to development of drug resistance, chronic osteomyelitis still an important cause of high morbidity. Better management of chronic osteomyelitis requires  In the present study, chronic osteomyelitis was common in the elderly age group, males are more affected than females between age group 11-60 years which is in correlation with the studies done by Mita D W et al 4 and Faria Malik et al. 14 In the present study most common predisposing factor is as a result of trauma (45%) associated with compound fracture more common followed by postoperative infections (22%), diabetes with vascular insufficiency (19%) as predisposing factor, (10%) had smoking and alcohol as predisposing factor and 4(4%) patients acquired infection following hematogenous spread.
Though antimicrobial therapy desirable in controlling the infection chronic osteomyelitis is treated surgically by sequestrectomy along with antibiotic therapy. The aim of surgical intervention done is to remove every piece of an infection complrtely and thereby attaining a viable and vascular environment.

Conclusion
Present study showed chronic osteomyelitis common in elderly age group as a sequel of trauma involving long bones commonly. The most commenest organism isolated was Staphylococcus aureus as predominant pathogen involved in chronic osteomyelitis followed by Pseudomonas aeruginosa among gram negative organisms. Prevalence of MRSA among Staphylococcus aureus and ESBL, MBL producers among gram negative organisms being high. Since resistant organisms are highly involved in chronic osteomyelitis, culture based antibiotic therapy helps in effectively treating the condition, also helps in preventing drug resistance.

Source of funding
None.

Conflict of interest
None.