Some exometabolites produced by basic representatives of human anaerobic microflora were investigated, detected by gas chromatography - mass spectrometry (GC-MS). In vitro besides lactic acid Bifidobacterium and Lactobacillus generate substantial amounts of phenyllactic and p-hydroxyphenyllactic acids. Clostridium produced 2-hydroxybutyric acid and to a lesser extent lactic and phenyllactic acids. In contrast to С. perfringens, C. sporogenes generates substantial amount of phenylpropionic and p-hydroxyphenylpropionic acids and less p-hydroxyphenyllactic acid. С. perfringens produced minor amounts of 2-hydroxyglutaric acid. Bacteroids are potent producers of succinic and fumaric acids; they also contribute to production of significant portion of lactic acid. E. lentum generate lactic, phenyllactic and succinic acids and form a characteristic only for ones (from studied microorganisms) 2-hydroxyhexanic and 2-hydroxy-3-methylbutyric acids.
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Keywords: GS-MS, metabolites, anaerobes
Citation:
Beloborodova N.V., Bairamov I.T., Olenin A.Yu., Fedotcheva N.I. (2011) Exometabolites of some anaerobic microorganisms of human microflora. Biomeditsinskaya Khimiya, 57(1), 95-105.
Beloborodova N.V. et al. Exometabolites of some anaerobic microorganisms of human microflora // Biomeditsinskaya Khimiya. - 2011. - V. 57. -N 1. - P. 95-105.
Beloborodova N.V. et al., "Exometabolites of some anaerobic microorganisms of human microflora." Biomeditsinskaya Khimiya 57.1 (2011): 95-105.
Beloborodova, N. V., Bairamov, I. T., Olenin, A. Yu., Fedotcheva, N. I. (2011). Exometabolites of some anaerobic microorganisms of human microflora. Biomeditsinskaya Khimiya, 57(1), 95-105.
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