Paper Titles

LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples
p.1

Occurrence of Microcystis aeruginosa Kütz. Water Blooms in a Eutrophic Pond of Chidambaram Taluk
p.11

Length and Weight Relationship and Growth Performance of Meretrix casta under Experimental Culture Condition in Three Different Estuarine Environment
p.16

Biomonitoring Tool: Morphological Anomalies in Zygnema sp. of Ganga River Bank
p.24

India’s Initiatives on Environmental Safeguarding - Sustainability
p.32

Trend Analysis of Climate Change in Chittagong Station in Bangladesh
p.42

Epidemiology of Salmonella and Salmonellosis
p.54

Distribution and Composition of Aquatic Macrophytes in Santhapettai Lake of Villupuram District in Tamil Nadu
p.74

Immunomodulatory Activity of Ethanolic Leaf Extract of Terminalia chebula
p.82

HomeInternational Letters of Natural SciencesInternational Letters of Natural Sciences Vol. 47LC Determination and Stability Assessment of...

LC Determination and Stability Assessment of Macrolide Antibiotics Azithromycin and Spiramycin in Bulk and Tablet Samples

Article Preview

Abstract:

Development and validation of rapid HPLC method for quantifying macrolide antibiotics azithromycin (AZI) and spiramycin (SPI) in bulk and tablet samples is described. Determination was performed on a reversed phase C₁₈ ODB column (250×4.6 nm I.D) at ambient temperature, and employing a UV-detection set at 210 nm. The mobile phase consists of acetonitrile –2-methyl-2-propanol–hydrogenphosphate buffer, pH 6.2, with 1.8% triethylamine (32:8: up to 100, v/v/v), delivered at a flow-rate of 1.1 mL min^-1. The assay is linear in concentration ranges of: 0.004–4.8 and 0.0003–1.2 mg mL⁻¹ for azithromycin and spiramycin, respectively, with detection limit of 0.02% for SPI and 0.03% for AZI. Recovery experiments revealed recovery of 98.51–100.82%. The applicability of this method in stability assessment studies is evaluated.

By email Full Text Pdf

Info:

Periodical:

International Letters of Natural Sciences (Volume 47)

Pages:

1-10

DOI:

https://doi.org/10.56431/p-so7039

Citation:

Cite this paper

Online since:

September 2015

Authors:

Abdelghani Mahmoudi*

Keywords:

Azithromycin, HPLC-UV, Spiramycin, Stability Study, Tablet Dosage Forms

Export:

RIS, BibTeX

Permissions:

Creative Commons CC BY 4.0

* - Corresponding Author

[1] J. Elks, C.R. Ganellin (Eds.), Dictionary of Drugs, Chapman and Hall, London, 1991.

[2] S. Omura (Ed.), Macrolide Antibiotics: Chemistry, Biology and Practice, Academic Press, Orlando, FL, 1984.

[3] A. Mahmoudi, R.E.-A. Fourar, M.S. Boukhechem, S. Zarkout, Int. J. Pharm. 491 (2015) 285–291.

[4] L.C. Vaucher, C.S. Paim, A.D. Lange, E.E.S. Schapoval, Int. J. Pharm. 366 (2009) 82–87.

[5] C. Stubbs, I. Kanfer, Int. J. Pharm. 63 (1990) 113–119.

[6] O. Farghaly, N. Mohamed, Talanta 62 (2004) 531–538.

[7] C. Boyer, K. Gaudin, T. Kauss, A. Gaubert, A. Boudis, J. Verschelden, M. Franc, J. Roussille, J. Boucher, P. Olliaro, N.J. White, P. Millet, J-P. Dubost, J. Pharm. Biomed. Anal. 67– 68 (2012) 10–15.

DOI: 10.1016/j.jpba.2012.04.009

[8] S. Ashour, R. Bayram, Spectrochim Acta Part A: Mol. Biomol. Spectrosc. 99 (2012) 74–80.

[9] P.Y. Khashaba, J. Pharm. Biomed. Anal. 27 (2002) 923–932.

[10] A.P. Kumar, J.H. Park, J. Chromatogr. A. 1218 (2011) 1314–1317.

[11] H.K. Chepkwony, A. Vermaelen, E. Roets, J. Hoogmartens. Chromatographia 54 (2001) 51–56.

[12] N. Furusawa, Talanta. 49 (1999) 461–465.

[13] Z.Y. Yang, L.Wang, X. Tang, J. Pharm. Biomed. Anal. 49 (2009) 811–815.

[14] F.N. Kamau, H.K. Chepkwony, J.K. Ngugi, E. Roets, J. Hoogmartens, J. Chromatogr. Sci. 40 (2002) 529–533.

[15] C. Leal, R. Codony, R. Compañó, M. Granados, M. D. Prat, J. Chromatogr. A. 910 (2001) 285 –290.

DOI: 10.1016/s0021-9673(00)01231-0

[16] M.J. González de la Huebra, U. Vincent, C. von Holst, J. Pharm. Biomed. Anal. 43 (2007) 1628–1637.

[17] E. Wilms, H. Trumpie, W. Veenendaal, D. Touwa, J. Chromatogr. B. 814 (2005) 37–42.

[18] G. Bahrami, S. Mirzae, A. Kiani, J. Chromatogr. B. 820 (2005) 277–281.

[19] M.J. González de la Huebra, U. Vincent, G. Bordin, A.R. Rodríguez, Anal. Chim. Acta. 503 (2004) 247–256.

[20] M. Horie, H. Takegami, K. Toya, H. Nakazawa, Anal. Chim. Acta. 492 (2003) 187–197.

[21] R.V.S. Nirogi, V.N. Kandikere, M. Shukla, K. Mudigonda, S. Maurya, R. Boosi, A. Yerramilli, Anal. Chim. Acta. 553 (2005) 1–8.

DOI: 10.1016/j.aca.2005.08.007

[22] Y. Shen, C. Yin, M. Su, J. Tu, J. Pharm. Biomed. Anal. 52 (2010) 99–104.

[23] R.V. Nirogi, V.N. Kandikere, M. Shukla, K. Mudigonda, S. Maurya, R. Boosi, A. Yerramilli, Anal. Chim. Acta 553 (2005) 1–8.

DOI: 10.1016/j.aca.2005.08.007

[24] United States Pharmacopoeia 32ed, United States Pharmacopeial Convention, Rockville, MD, 2009, p.2292.

[25] British Pharmacopoeia, fifth ed., Her Majesty's Stationery Office Ltd., London, 2011.

[26] International Conference on Harmonization (ICH) of Technical Requirements for registration of Pharmaceuticals for Human Use Topic Q2 (R1): Validation of Analytical Procedures: Text and Methodology, Geneva, 2005.

[27] P. Hubert, J.J. Ngyen-Huu, B. Boulanger, E. Chapuzet, P. Chiap, N. Cohen, P.A. Compagnon, W. Dewe, M. Feinberg, M. Lallier, M. Laurentie, N. Mercier, G. Muzard, C. Nivet, L. Valat, Validation des procédures analytiques quantitatives: Harmonisation des démarches, STP. Pharma Pratiques. 13 (2003) 101–138.

DOI: 10.1016/j.jpba.2004.07.027