Development of New Drought Tolerant Breeding Lines for Vietnam Using Marker-Assisted Backcrossing

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Development of drought tolerant high-yielding varieties is essential because increased areas are subject to drought in the Mekong delta, Vietnam. The purpose of this experiment was developed using IR75499-73-1-B as drought tolerant donor and OMCS2000 as a recipient parent basis of a phenotypic and molecular marker for BC2F2 generation. Seven markers (RM219, RM201 RM105, RM23602, RM23877, RM24103 and RM328) were used for a idifying to drought tolerant. Primer RM23877 detected the highest number of lines as homozygous donor alleles (11 lines), followed by RM105 and RM201 (9 lines). The drought gene was introgressed into the new breeding lines. The plant height, number of tillers, and filled grain had positive correlation with yield/hill under drought stress. The lines BC2F2-45 and BC2F2-54 developed as drought tolerant, and gave high yield. This is an opportunity to improve breeding for high yield and drought tolerant rice varieties in Vietnam.

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October 2016

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[1] E. A. Bray, J. Bailey-Serres, E. Weretilnyk, Responses to abiotic stresses, in B. Buchannan, W. Gruissem, R. Jones, (Eds), Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists. Rockville, MD, 2000, p.1158–1249.

Google Scholar

[2] B.C.Y. Collard, D.J. McKill, Marker-assisted selection: an approach for precision plant breeding in the twenty-first century, Philosophical Transactions of the Royal Society B: Biological Sciences. 363(1491) (2008) 557–572.

DOI: 10.1098/rstb.2007.2170

Google Scholar

[3] J.C. O'Toole, Rice and Water: The Final Frontier. In Proceedings of the First International Conference on Rice for the Future, Bangkok, Thailand, 31 August–2 September, 2004.

Google Scholar

[4] J. Bernier et al., A large-effect QTL for grain yield under reproductive-stage drought stress in upland rice, Crop Sci. 47 (2007) 507-518.

DOI: 10.2135/cropsci2006.07.0495

Google Scholar

[5] R. Venuprasad et al., Identification and characterization of large-effect quantitative trait loci for grain yield under lowland drought stress in rice using bulk-segregant analysis, Theor. Appl. Genet. 120 (2009) 177-190.

DOI: 10.1007/s00122-009-1168-1

Google Scholar

[6] N.T. Lang et al., Quantitative Trait Loci (QTLs) Associated with Drought Tolerance in Rice (Oryza Sativa L.), SABRAO Journal of Breeding and Genetics. 45(3) (2013) 409-421.

Google Scholar

[7] S.B. Verulkar et al., Breeding resilient and productive genotype adapted to drought-prone rainfed ecosystem of India, Field Scrops Res. 117 (2010) 197-208.

DOI: 10.1016/j.fcr.2010.03.005

Google Scholar

[8] J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular cloning: a laboratory Manual, vol. 3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989.

DOI: 10.1002/jobm.19840240107

Google Scholar

[9] S. R. McCouch et al., Microsettlite marker development, mapping and application in rice genetics and breeding, Plant Mol Biol. 35 (1997) 89-99.

Google Scholar

[10] S. Temnykh et al., Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.), Theor. Appl. Genet. 100 (2000) 697-712.

DOI: 10.1007/s001220051342

Google Scholar

[11] IRRI. Standard Evaluation System for rice. International Rice Research Institute, Los Banos, Philippines, 1996.

Google Scholar

[12] R.M.D. Koebner, R.W. Summers, 21st century wheat breeding: plot selection or plate detection, Trends Biotech. 21 (2003) 59–63.

DOI: 10.1016/s0167-7799(02)00036-7

Google Scholar

[13] D.G. Bonnett, G.J. Rebetzke, W. Spielmeyer, Strategies for Efficient Implementation of Molecular Markers in Wheat Breeding, Molecular Breeding. 15 (2005) 75-85.

DOI: 10.1007/s11032-004-2734-5

Google Scholar

[14] X. Yongzhong, Z. Qifa, Genetic and Molecular Bases of Rice Yield, Plant Biol. 61 (2010) 421–442.

Google Scholar

[15] Y. Huang et al., Heterosis and polymorphisms of gene expression in an elite rice hybrid as revealed by a microarray analysis of 9198 unique ESTs, Plant Mol. Biol. 62 (2006) 579–591.

DOI: 10.1007/s11103-006-9040-z

Google Scholar