STUDY OF THE INTERACTION BETWEEN E. AMYLOVORA AND PLANT TISSUE THROUGH MEASUREMENT OF ELECTROLYTE LEAKAGE

**STUDY OF THE INTERACTION BETWEEN E. AMYLOVORA AND PLANT TISSUE THROUGH MEASUREMENT OF ELECTROLYTE LEAKAGE**

Authors:	M.-N. BRISSET, J.-P. PAULIN
DOI:	10.17660/ActaHortic.1990.273.26
Abstract: The measurement of electrolyte leakages from plant tissue allows the caracterization of different plant-bacteria interactions. After vacuum infiltration of leaf discs with a bacterial suspension, leaf discs were incubated in suitable buffered conditions and the electrolyte leakages were monitored from time to time during 48 hours. Different patterns of leakage were obtained depending on the type of plant bacteria interaction. We were able to distinguish compatible situations (Erwinia amylovora/pear, Pseudomonas syringae pv. phaseolicola/bean) from incompatible situations (Erwinia amylovora/bean, Pseudomonas syringae pv. phaseolicola/pear) or indifferent situation (Erwinia herbicola on both). In the case of fire blight, electrolyte leakage assessment could be a convenient technique for several purposes : study of aggressiveness of strains (wild-types strains or mutants), study of susceptibilities of plant genotypes.
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