ISHS Acta Horticulturae 336: II International Symposium on In Vitro Culture and Horticultural Breeding
DEVELOPMENT OF NUCELLAR SOMATIC EMBRYOS OF THEOBROMA CACAO |
| Authors: | A. Figueira, J. Janick |
| Keywords: | Carbon dioxide, cocoa |
| DOI: | 10.17660/ActaHortic.1993.336.29 |
| Abstract:
Somatic embryogenesis in cacao (Theobroma cacao L.) derived from immature zygotic embryos has limited value for propagation, because the zygote is an untested genotype. Further, conversion of somatic embryos into seedlings has been marginal. Recently, somatic embryogenesis has been reported from nucellar tissue, which is maternal in origin. We have confirmed the induction of nucellar embryony and developed a protocol for conversion of nucellar somatic embryos into seedlings that involves preculture of somatic embryos in liquid medium and transfer to semi-solid medium in chambers receiving 20,000 ppm carbon dioxide | |
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