ISHS Acta Horticulturae 829: VI International Symposium on In Vitro Culture and Horticultural Breeding
IN VITRO FLOWERING AND PROPAGATION OF BULBOPHYLLUM AURICOMUM LINDL., THE ROYAL FLOWER OF MYANMAR |
| Authors: | M.M.M. Than, A. Pal, S. Jha |
| DOI: | 10.17660/ActaHortic.2009.829.14 |
| Abstract:
Reproducible protocols for micropropagation of B. auricomum Lindl. were developed in the present study. Seeds from three-month-old immature seed capsules were aseptically cultured on Murashige and Skoog’s (MS) and modified Knudson C (KC) medium. MS medium was found to be the most effective culture medium for seed germination, and a high rate of protocorms formation, multiplication, and differentiation into seedlings. The combination of 1.0 mg/L 6-Benzylaminopurine (BAP) with 0.5 mg/L α-Naphthaleneacetic acid (NAA) induced maximum number of multiple shoots (15.0±1.7) and optimal rooting (3.0±0.7) of plantlets was obtained in 1.0 mg/L kinetin (KIN) and NAA 2.0 mg/L. In vitro flowering was observed within six months after seed sowing, with the highest percentage (50±15.8%) of flowering observed in MS medium with 1.0 mg/L BAP + 0.5 mg/L NAA; with 2.0 mg/L BAP + 1.0 mg/L NAA or with 0.5 mg/L BAP + 2.0 mg/L NAA. In vitro-developed flowers from different treatments were dissected and compared with flowers of the field grown plants. | |
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