Effect of acetylation on arthropathic activity of group A streptococcal peptidoglycan-polysaccharide fragments.
Public Deposited- Creator
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Stimpson, Stephen A.
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Lerch, Robert A.
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Cleland, David R.
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Yarnall, David P.
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Clark, Richard L.
- Affiliation: School of Medicine, Department of Radiology
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Cromartie, William J.
- Affiliation: School of Medicine, Department of Microbiology and Immunology
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Schwab, John H.
- Affiliation: School of Medicine, Department of Microbiology and Immunology
- Abstract
- Purified group A streptococcal peptidoglycan-polysaccharide (PG-PS) fragments were either de-O-acylated, or acetylated and then de-O-acylated to yield N-acetylated PG-PS. Native PG-PS was poorly degraded, N-acetylated PG-PS was extensively degraded, and de-O-acylated PG-PS was only slightly degraded by hen egg white lysozyme. N-acetylated PG-PS was also extensively degraded by human lysozyme and partially degraded by rat serum or rat liver extract. After a single intraperitoneal injection of rats with a sterile, aqueous suspension, all PG-PS preparations induced acute arthritis. The acute arthritis induced by N-acetylated PG-PS was significantly more severe than that induced by native PG-PS; that induced by de-O-acylated PG-PS was of intermediate severity. After the acute reaction, rats injected with native PG-PS developed chronic relapsing erosive synovitis which remained severe for the duration of the experiment (83 days). In contrast, joint inflammation induced by N-acetylated PG-PS resolved within 6 weeks with little evidence of recurrent disease. Chronic arthritis induced by de-O-acylated PG-PS was of intermediate severity. In another assay of arthropathic activity, the arthritis in all rat ankle joints, which had been injected directly with native PG-PS, could be reactivated 3 weeks later by the intravenous injection of a small dose of PG. In contrast, only 50% of the joints initially injected with de-O-acylated PG-PS and none of the joints injected with N-acetylated PG-PS could be reactivated. These studies support the concepts that the resistance of PG-PS to muralytic digestion is crucial for chronic arthropathic activity and that the nature and degree of PG acetylation are important molecular determinants of the phlogistic activities of PG-PS polymers.
- Date of publication
- 1987
- Keyword
- Souris
- Peptidoglycan
- Polymère
- Mammalia
- Constituant cellulaire
- Voie parentérale
- Inflammation
- Vertebrata
- Experimental disease
- Rodentia
- Peptidoglycane
- Acétylation
- Polyoside
- Streptococcus A
- Experimental animal
- Acetylation
- Mouse
- Animal expérience
- Parenteral administration
- Streptococcaceae
- Polymer
- Arthropathy
- Pathologie expérimentale
- Arthropathie
- Cell component
- Bacteria
- DOI
- Identifier
- PMID: 3539800
- PMCID: PMC260274
- Onescience id: c23148c486ff9f0e7f34666cbe9dc0d38f22f005
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Infection and Immunity
- Journal volume
- 55
- Journal issue
- 1
- Page start
- 16
- Page end
- 23
- Language
- English
- Version
- Publisher
- ISSN
- 0019-9567
- 1098-5522
- 1070-6313
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- Parents:
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