On line Fir st A rtic le Evaluating Varying Calf Milk Replacers for Optimum Growth Performance in Salt Range Lambs

Muhammad Fiaz1, Khadija Munawar2, Muhammad Mushtaq2,*, Mukhtar Ahmad3, Mushtaq Ahmad Chaudhry3, Muhammad Aslam3, Tanveer Ahmad1 and Nasrullah4 1Department of Livestock Production and Management, PMASArid Agriculture University, Rawalpindi, Punjab, Pakistan 2Department of Zoology, PMAS-Arid Agriculture University, Rawalpindi, Punjab, Pakistan 3Barani Livestock Production Research Institute, Kherimurat, District Attock, Punjab, Pakistan 4Faculty of Veterinary and Animal Sciences, Lasbela University of Agriculture Water and Marine Sciences, Uthal, Balochistan, Pakistan Article Information Received 24 August 2015 Revised 20 March 2016 Accepted 07 April 2017 Available online 06 September 2017

S heep is raised primarily for mutton production in the country and secondarily for carpet type wool production.The major Sheep breeds in province of Punjab are thin tailed like Buchi, Cholistani, Kajli, Lohi and Sipli.However, Salt Range is the only fat tailed breed available in Punjab and particularly in Pothowar region.
Salt Range performance is badly affected during scarcity period when feed resources are minimized as region is totally rain fed.Consequently, it is quite difficult for ewes to nourish their newly born lambs especially during lambing season.The suboptimal nutrition in ewes might affect growth of mammary secretary tissue (Jenkinson, 2003;Karabacak et al., 2016;Knight and Sorensen, 2001) during pregnancy which leads to reduced milk yield and ultimately lambs survival (Binns et al., 2002).Unfortunately enormous numbers of newly born lambs die annually (Iqbal et al., 1993) and more than 66 percent of lamb died before, during and shortly after birth (Berger, 1997).The mortality in lambs at early age in Punjab was due to starvation, chilling, dystocia and mis-mothering (Mustafa et al., 2014).
The early lamb mortality eventually reduces the mutton production in country and accounts greater loss to the meat industry.As an attempt to reduce early lamb mortality, newly born lambs can be reared successfully on alternative source of mother milk that is milk replacer (Emsen et al., 2004).Literature regarding use of milk replacer is limited and milk replacers for lambs are not available in market.However, lambs can be reared satisfactorily on artificial calf milk replacer having adequate nutrients like protein and energy (Mc Kusick et al., 2001;Sevi et al., 2001).Previously, Ocak and Cankaya (2013) reported that calf milk replacer might be used for successful rearing of lambs even at lower cost than ewe milk.Keeping in view the crucial importance of early lamb mortality the present study was designed with aim to

A B S T R A C T O n l i n e F i r s t A r t i c l e
feed newly born Salt Range lambs with calf milk replacers for optimum growth performance and survivability.

Materials and methods
The research site Barani Livestock Production Research Institute (BLPRI) Kherimurat is situated in Fateh Jang, Attock, Punjab, Pakistan.This institute is included in home tract of Salt Range sheep.Its geographical coordinates are 33° 33' 57" North, 72° 38' 57" East.The institute is located at a distance of 50 km from Islamabadcapital of Pakistan.The range of ambient temperature during year of 2015 was 10 to 20° C, whereas average precipitation was 7.8 mm (World Weather Online, 2015).
Twenty four newly born lambs having similar body weight and size were selected from the flock of Salt Range sheep maintained at BLPRI Kherimurat.The selected lambs were initially fed with Colostrum for 07 days and later-on were allocated randomly to 03 treatment groups having 08 lambs each.
The lambs in treatment group-1 were given natural ewe milk (Control) and in group-2 were fed alternative source of mother milk i.e. calf milk replacer-1 (Telilac), whereas lambs in treatment group 3 were fed another calf milk replacer-2 (Creamo).The Telilac was constituted of protein 24.5% and fat 21%, whereas Creamo had protein 20.5% and Fat 21%.This dietary treatment was continued for 45 days including adjustment period of 07 days.
The feeding of milk replacer was given individually @ 10% of body weight twice morning and evening daily.The 100 g milk powder was added in 600 ml hot water for making liquid diet.The water was heated up to 50° C before mixing and then added powder was thoroughly mixed with continuous stirring.The liquid milk replacer was fed to lambs at 40° C through nipple feeding.All utensils needed and nipples were thoroughly cleaned with detergent powder and then boiled water.The washed nipples and utensils were then air dried before used.Hand gloves were used while touching and handling of nipples and utensils.All experimental lambs were also offered ad lib Lamb starter ration having CP 18.5% and TDN 80%.The composition of starter solid diet is soybean meal, 26%; maize grain broken, 40%; rice broken, 20%; wheat bran, 08%; molasses, 05% and minerals mixture, 01% (CP, 18.58%; TDN, 80.54%).The fresh oat fodder (CP, 9.6%) was also offered at the choice of young lambs in all three groups.
The performance of lambs was determined by average daily gain (ADG), feed conversion ratio (FCR), survival rate, body measurements (gain in body height, length and heart girth) and cost of milk per kg live weight gain.The milk or milk replacer intake was recorded daily, whereas weight gain was recorded weekly along with body measurements.Number of mortality was noted daily, whereas feed conversion ratio and milk cost per kg weight gain were calculated.The collected data were analyzed using ANOVA techniques (Steel et al., 1997) under completely randomized design (CRD).The difference among treatments mean was tested through Duncan Multiple Range Test (Duncan, 1955).The statistical model used was as under: Where, Y ij is observation on i th treatment due to j th animal, μ is overall mean, τ i is effect of i th treatment (Σ τi = 0 and i = 1, 2, 3) and ε ij is random error associated with i th treatment with the restriction that variance and σ 2 and mean zero.

Results and discussion
The performance of Salt Range lambs kept under different treatment groups at Barani Livestock Production Research Institute is shown in Table I.

O n l i n e F i r s t A r t i c l e
ADG in Salt Range lambs fed natural ewe milk was higher (P<0.05)than those fed calf milk replacer-2 Creamo, though not different (P>0.05) from lambs fed calf milk replacer-I Telilac (Table I).The FCR of natural ewe milk is also not different (P>0.05) with calf milk replacer-I Telilac but lower (P<0.05)than calf milk replacer-2 Creamo (Table I).
The findings of this study are supported by those of Ocak and Cankaya (2013).Similarly, Emsen et al. (2004) reported successful rearing of Awassi lambs on calf milk replacer, and other workers (Galina et al., 1995;Delgado-Pertinez et al., 2009) observed similar findings even in goat kids fed milk replacer.Previously, Xi-Chun et al.
(2010) reported no differences of body length, height and chest measurement (P>0.05)among lambs fed ewe milk and milk replacers.No difference in improvement of body measurements might be due to factor that milk replacer diet had adequate protein and energy.Whereas less improvement in body measurements of lambs fed calf milk replacer-2 might be due to lower concentration of protein in the replacer.However, Rodrigues et al. (2008) reported differently that lambs belonging to Assaf breed fed natural ewe milk gained greater than of those fed artificial milk.The possible reason for this divergence might be different breed of lambs (Assaf Lambs) in that study.
In present study, poor performance of lambs fed milk replacer-2 (Creamo) might be due to the factor that it had lower dietary protein (CP 20.5%) concentration as compared to that (CP 24.5%) of calf milk replacer-1 (Telilac).The reduced dietary protein level significantly affect growth performance of lambs (NRC, 1985).Provision of dense dietary protein might be a better strategy for optimum growth (Arthington and Kalmbacher, 2003).It was inferred that high quality calf milk replacer can be used in Salt Range lambs as substitute of ewe milk.The survival rate was 100% in lambs fed ewe milk and calf milk replacer-1 (Telilac), whereas it was 75% in group of lambs fed calf milk replacer-2 (creamo).
Findings of present study regarding zero mortality in lambs fed calf milk replcer-1 were strengthened by report of Delgado-Pertinez et al. (2009) who also reported no mortality.Similarly, Ocak and Cankaya (2013) also affirmed that lambs can be successfully raised with calf milk replacer even with better survival rate (91.7%) as compared to 55% in ewe reared lambs.The comparative better survivability in calf milk replacer-1 than calf milk replacer-2 might be attributed to higher concentration of protein in Telilac.The dietary protein was needed for optimized growth and feed efficiency which can also generally increase immunocompetence (Latshaw, 1991) and ultimately improved survivability of lambs.
Besides that gain in the body height and length of lambs fed ewe milk and calf milk replacer-1 (Telilac) were not different (P>0.05),though it was higher in animals fed on calf milk replacer-2 (Creamo).The gain in heart girth was however, found highest in ewe milk followed by calf milk replacer-1 and calf milk replacer-2.Table I also shows the cost of milk calf fed on milk replacer-1 and calf milk replacer-2 which was almost the same but lower than ewe milk.
Findings regarding cost in this study were also endorsed by few workers previously (Galina et al., 1995;Emsen et al., 2004;Ocak and Cankaya, 2013;Delgado-Pertinez et al., 2009).They reported that lambs could be reared successfully on artificial milk replacers on lower cost.It was inferred that optimum survivability in Salt Range lambs fed high quality calf milk replacer can be achieved with low milk cost.The comparative lower cost of calf milk replacer might be due to cheaper synthetic constituents used in preparation of milk replacer powder.

Conclusion
It was concluded from findings of present study that high quality calf milk replacer can be used as an efficient substitute of ewe milk for optimum growth performance in neonatal Salt Range lambs after mandatory colostrum feeding.

Table I .-Effect of feeding natural ewe milk and calf milk replacers on average daily gain, feed efficiency and milk cost per kg gain survivability, body height, length and girth gains in Salt Range lambs.
All values are in Mean±SEM; *, variables having different superscripts in a row are different (P<0.05);**, milk cost per kg gain was calculated by dividing cost of milk consumed over weight gain in Kg.