Breeding and Mortality of Royal Bengal Tiger (Panthera tigris tigris Linnaeus 1758) at Bahawalpur Zoo, Punjab, Pakistan

Ahmad Ali1,*, Sheikh Muhammad Azam2, Khalid Javed Iqbal1, Ghulam Mustafa1 and Mujahid Kaleem3 1Department of Life Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan 2Department of Zoology, University of Education, Lahore, DG Khan Campus, Pakistan 3Punjab Wildlife & Parks Department, 2-Sanda Road, Lahore, Punjab, Pakistan Article Information Received 22 February 2016 Revised 05 May 2016 Accepted 21 November 2016 Available online 08 June 2017

Study was conducted on breeding and mortality of Royal Bengal Tiger (Panthera tigris tigris Linnaeus 1758). There are five big cat species in the genus Panthera and tiger is the biggest one. Data were collected by recording observations on breeding of Royal Bengal Tiger in captivity at Bahawalpur Zoo from June 2012 to July 2014 (two years) on prescribe sheets. Data from June, 2003 to December, 2011 (nine years) were collected from zoo management. In two years study Tiger breed once and produce four cubs (female) while in nine years it breed three times; first three cubs (one male and two female), two and one cubs were produced in 2003, 2005 and 2006, respectively. Six days estrous cycle and 107 days gestation period was observed in tigress. Mortality of one cub of 32 days in August, 2013 and second cub in Jannaury, 2014 of 173 days was observed due to congenital abnormality of liver. Parent tigress (Rani) was also died in May, 2014 due to respiratory failure and cardiac arrest and female cub died in 2005 due to canabalism. T here are five big cat species in the genus Panthera, the lion (Panthera leo), snow leopard (Panthera uncia), leopard (Panthera pardus), tiger (Panthera tigris) and jaguar (Panthera onca) (Davis et al., 2010). Tiger Panthera tigiris Linnaeus 1758 is the biggest member of family felidae. Eight living sub-species of tiger have been recognized (Mazak, 1979). The type sub species Royal Bengal Tiger Panthera tigiris tigiris is ranked as endangered since 2010 IUCN red list with decreasing trend in its population (IUCN, 2011). In the wild Royal Bengal Tiger, sexual maturity is attained at three to four years in females, and four to five year in males (Mazak, 1965(Mazak, , 1979. In captivity, maximum age of tiger has been recorded twenty to twenty six years; this may also be near about their age in the wild (Mazak, 1965(Mazak, , 1979. Tiger mating may occure throghout the year; however this is most likely to occur from November to April (Kaplanov, 1948;Locke, 1954;Corbett, 1961;Stroganov, * Corresponding author: ahmads1976pk@yahoo.com 0030-9923/2017/0004-0001 $ 9.00/0 Copyright 2017 Zoological Society of Pakistan 1969; Sankhala, 1967Sankhala, , 1978Geptner and Sludskij, 1972). Tiger female is receptive for three to six days and comes to oestrous at intervals of about three to nine weeks (Mazak, 1967). Gestation period ranges between 96 to 111 days, while 104 to 106 days being the optimum (Mazak, 1979). Usually the litter is two or three and mostly in range of one to four (Mazak, 1979); in exceptional cases six cubs are also noticed (Sankhala, 1967(Sankhala, , 1978. Tigress has been noticed making a roofed nest for offsprings with hair, dry grass and leaves (Abramov and Pikunov, 1976). The interval between offsprings born is usually twenty to fourty minutes (Sankhala, 1967). The mean yearly growth frequency has been estimated to be up to six percent of all the tiger populations to reach the double number of tigers in twelve years to fulfill the Global requirement that would be a noble but seems impossible (Miquelle et al., 2015).
Most adult tigers die evidently having been poisoned, shot or snared by the humans than of anyother cause. In exceptional cases some large ungulates fatally wound a tiger (Mazak, 1979). Moreover, a group of elephants or cave buffaloes can take life of tiger. Reportedly encounters with porcupines also occasionally result in serious injuries

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and fatality of tigers. The tiger cubs have to face excessive dangers as compared to the adults, including being killed by full grown tiger males. Fifty percent survival ratio has been considered the highest (Corbett, 1961;Krumbiegel, 1955). In Pakistan, for the local threatened status of carnivores, it is the more important to properly manage the wild ungulate and predator populations to help conserve the population of wild carnivores in general (Anwar et al., 2012). There is scanty information about the diseases of tiger in the wild; only two cases of rabies have been reported from India (Mazak, 1979). The tiger is of course, extinct in Pakistan but it should be a sobering thought that it has only become so within the last seventy years, in a region which cradled man's civilization for over 4000 years. The last survivor in Sindh, a tigeress was shot in 1886 by Col. Mchae. The late Amir of Bahawalpur, H.H. Sir Sadiq Muhammad Khan Abbasi, related how his father had shot thirteen tigers within Bahawalpur state territory in the Indus riverine jungles and that the last specimen was shot by him in 1906 a few miles below Panjnad. At that time Indus River was surrounded by a continuous belt from four to twelve miles wide of Tamarix dioica and Sacharum bengalensis forest (Roberts, 1997).
At Bahawalpur Zoo, the study site, only one breeding pair was being maintained in captivity during the study period from June 2012 to July 2014. The major factors that impose us to start this work are poor conservation stratigies as not a single effort made by any governmental or NGO to save this species. Moreover; the natural beauty of this fascinating species and its ecological role as predator in ecosystem are also indusive factors to study this species. Furthermore, short gestation period allows studying more progenies in a short time period. No significant work has been done in Pakistan on breeding and mortality of this big cat in captivity prior to this work. The present study was initiated to understand the breeding cycle and mortality of the tiger in captivity that may help for the rehabilitation in his wild ecosystem and also useful for commercial purposes like as pet or in public shows. Further it is our responsibility to save this species for our future generation.

Material and methods
Bahawalpur (N 29.3956°, E 71.6836°), now a large city has been a part of Cholistan desert, where the tiger was once naturally distributed. The average rainfall in Bahawalpur is 20 to 25 cm annually. Tiger breeding data spanned over eleven years since July 2003 to June 2014 has been analysed for this study. Data for two years, from July 2012 to June 2014 were collected by our research team by recording direct observations while the nine years data since July 2003 to June 2012 were clollected from the Zoo management (Wildlife and Parks Department of Punjab). During pregnancy a close watch was kept on female's behavior and only one keeper would attend the female to reduce the disturbance level. Gestation period and congenital defects were also recorded. Cubs or adults mortality causes were examined by postmortem examination with the help of veternarian. Data analysis and Graphs were made using Excel 2007.

Results and discussion
The age of parent tigress was 13 years while male parent tiger was 9 years old at the start of the study i.e., July 2012. Tiger bred only once during study period and produced four female cubs (Table I). Previous data (2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011) showed that that the pair bred for the first time in 2003 when three cubs (one male and two female) were produced. In 2005 and 2006 two (one male and one female and one (female) cubs were born, respectively. However, breeding was not recorded during the period 2007 to 2012 (Fig. 1). According to Sunquist (1981) the captive tigeress breeds mostly from two to sixteen years of age however maximum litters are produced when her age is above 6.2 years.

O n l i n e F i r s t A r t i c l e
Very little information is available on these aspects in wild tiger populations. Sunquist (1981) once observed the oestrus in a thirty month old female that was perhaps the earliest. Sunquist (1981) also observed that in wild tiger populations litter size ranged from one to seven cubs.
During present study six days esterous cycle and 107 days gestation period was observed in the tigeress (Table I), while Mazak (1979) reported three to six days of esterous cycle and 104 days of gestation in tigeress. Further he reported that gestation period ranged from 96 days to 111 days.
In our study mating was observed in the month of March (Table I) while several scientists stated that mating in Tigers may occure throghout the year; however this was most likely to happen from November to the April (Kaplanov, 1948;Locke, 1954;Corbett, 1961;Stroganov, 1969;Sankhala, 1967Sankhala, , 1978Geptner and Sludskij, 1972).
It was observed that one cub of 32 days in 2013 while second cub in 2014 of 173 days died due to congenital abnormality of liver. Parent tigress (Rani) also died in 2014 due to respiratory failure and cardiac arrest (Table II); and another female cub had died in 2005 due to canabalism. No death of male tiger has been reported during the period 2003 to 2014 (Fig. 1). Cavaliere in 2015 reported that the Seigfried and Roy's white lion died due to cardiac and respiratory arrest.

Aknowledgement
Authors are grateful to the management of Bahawalpur Zoo for their cooperation and providing us access to the previous data on tigers. We also grateful to the attendant of the Zoo for handling the tigers during observation and data collection.

Statement of conflict of interest
Authors have declared no conflict of interest.