Cytotoxicity evaluation of CELNORM, a nutritional health supplement, on MCF7 breast cancer cells

T N Sathya; Vijay A Mehta; Devi Senthil; K R Navaneethakrishnan; S S Murugan; T S Kumaravel

doi:10.17485/IJST/v13i30.1150

Article

Cytotoxicity evaluation of CELNORM, a nutritional health supplement, on MCF7 breast cancer cells

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Indian Journal of Science and Technology

DOI: 10.17485/IJST/v13i30.1150

Year: 2020, Volume: 13, Issue: 30, Pages: 3070-3075

Original Article

Cytotoxicity evaluation of CELNORM, a nutritional health supplement, on MCF7 breast cancer cells

T N Sathya¹, Vijay A Mehta², Devi Senthil³, K R Navaneethakrishnan⁴, S S Murugan⁵, T S Kumaravel^6*

¹Regulatory Toxicologist, GLR Laboratories Private Limited, #444, Gokulam street, Mathur, Chennai. PIN – 600068
²Founder and Chairman, Retort Pharmaceuticals Private Limited, #21/2, McNichols Road, Chetpet, Chennai, PIN -600031
³Group Product Manager, Retort Pharmaceuticals Private Limited, #21/2, McNichols Road, Chetpet, Chennai, PIN -600031
⁴Assistant Director, GLR Laboratories Private Limited, #444, Gokulam street, Mathur, Chennai. PIN – 600068
⁵Managing Director, Department of Toxicology, GLR Laboratories Private Limited, #444, Gokulam street, Mathur, Chennai. PIN – 600068
⁶Chairman, GLR Laboratories Private Limited, and Head of Toxicology and Risk Assessment., GLR Laboratories Private Limited, #444, Gokulam street, Mathur, Chennai. PIN – 600068

*Corresponding Author
Email: [email protected]

Received Date:14 July 2020, Accepted Date:04 August 2020, Published Date:19 August 2020

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objective: To evaluate the in vitro cytotoxic potential of CELNORM, a nutritional health supplement, on MCF7 human adenocarcinoma breast cancer cells. Methods: Briefly, MCF7 cells were cultured for 24 hours in complete growth medium containing fetal bovine serum and antibiotics and exposed to various concentrations (500, 250, 125, 62.5, 31.25, 15.625, 7.8125, 3.906 micrograms/mL) of CELNORM for a period of 24 hours. Cell viability/death was assessed qualitatively by microscopy and quantitatively by MTT assay. Findings: CELNORM exhibited significant cytotoxic effect on MCF-7 cells in a dose dependent manner. Under microscopy, there was an evident, dosedependent increase in the number of apoptotic cells. Cell viability exhibited in terms of percentage viability, fell below 70% from 7.9 mg/mL, which is indicative of cytotoxic response from this dose. The IC50 value was determined to be 43.51 mg/mL. Applications: With such an effective combination of natural ingredients, the results are promising. Therefore, we conclude that CELNORM can be a useful option in the supplemental therapy for breast cancer treatment in the panel of Complementary and Alternative Medicine (CAM).

Keywords: CELNORM; breast cancer; cytotoxicity; MCF7; complementary and alternative medicine

References

Rossi RE, Pericleous M, Mandair D, Whyand T, Caplin ME. The role of dietary factors in prevention and progression of breast cancer. Anticancer Research. 2014;34(12):6861–6875.
Sorlie T, Perou CM, Tibshirani R, Aas T, Geisler S, Johnsen H, et al. Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications. Proceedings of the National Academy of Sciences. 2001;98(19):10869–10874. Available from: https://dx.doi.org/10.1073/pnas.191367098
Azamjah N, Soltan-Zadeh Y, Zayeri F. Global Trend of Breast Cancer Mortality Rate: A 25-Year Study. Asian Pacific Journal of Cancer Prevention. 2019;20(7):2015–2020. Available from: https://dx.doi.org/10.31557/apjcp.2019.20.7.2015
Lopes CM, Dourado A, Oliveira R. Phytotherapy and Nutritional Supplements on Breast Cancer. BioMed Research International. 2017;2017:1–42. Available from: https://dx.doi.org/10.1155/2017/7207983
Complementary, Alternative, or Integrative Health: What’s in a Name? Available from: https://nccih.nih.gov/health/integrativehealth
Nagykálnai T, Landherr L, Nagy AC. Vitamin D and breast cancer. Orvosi Hetilap. 2014;155(28):1091–1096. Available from: https://dx.doi.org/10.1556/oh.2014.29906
Hassan F, Mohammed SAAQ, Philip A, Hameed AA, Yousif E. Gold (III) Complexes as Breast Cancer Drug. Systematic Reviews in Pharmacy. 2017;8(1):76–79. Available from: https://dx.doi.org/10.5530/srp.2017.1.13
Hassan F, El-Hiti G, Abd-Allateef M, Yousif E. Cytotoxicity anticancer activities of anastrozole against breast, liver hepatocellular, and prostate cancer cells. Saudi Medical Journal. 2017;38(4):359–365. Available from: https://dx.doi.org/10.15537/smj.2017.4.17061
Hassan F, Hairunisa N, Mohammed SA, Yousif E. A Study on Antitumor Effect of 1,3,4-Thiadiazole Derivatives in Prostate and Breast Cancer Cell Lines (In Vitro) 2017.
Razak NA, Abu N, Ho WY, Zamberi NR, Tan SW, Alitheen NB, et al. Cytotoxicity of eupatorin in MCF-7 and MDA-MB-231 human breast cancer cells via cell cycle arrest, anti-angiogenesis and induction of apoptosis. Scientific Reports. 2019;9(1). Available from: https://dx.doi.org/10.1038/s41598-018-37796-w
Geran RI, Greenberg NH, Macdonald MM, Shumacher AM, Abbott BJ. Protocols for screening chemical agents and natural products against animal tumors and other biological systems. Cancer Chemotherapy Reports, Part III. 1972;3:1–103.
Kubatka P, Liskova A, Kello M, Mojzis J, Solar P, Solarova Z, et al. Plant-derived functional foods with chemopreventive and therapeutic potential against breast cancer: A review of the preclinical and clinical data. In: Kabir Y., ed. Functional Foods in Cancer Prevention and Therapy. (pp. 283-314) Academic Press. 2020.
Hu S, Xu Y, Meng L, Huang L, Sun H. Curcumin inhibits proliferation and promotes apoptosis of breast cancer cells. Experimental and Therapeutic Medicine. 2018;16(2):1266–1272. Available from: https://doi.org/10.3892/etm.2018.6345
Liu J, Pan Y, Chen O, Luan Y, Xue X, Zhao J, et al. Curcumin inhibits MCF-7 cells by modulating the NF-κB signaling pathway. Oncology Letters. 2017;14(5):5581–5584.
Liu H, Ho Y. Anticancer effect of curcumin on breast cancer and stem cells. Food science and human wellness. 2018;7:134–137. Available from: https://doi.org/10.3892/ol.2017.6860
Kakarala M, Brenner DE, Korkaya H, Cheng C, Tazi K, Ginestier C, et al. Targeting breast stem cells with the cancer preventive compounds curcumin and piperine. Breast Cancer Research and Treatment. 2010;122(3):777–785. Available from: https://dx.doi.org/10.1007/s10549-009-0612-x
Chen H, Shao F, Zhang F, Miao Q. Association between dietary carrot intake and breast cancer A meta-analysis. Medicine (Baltimore). 2018;97(37). Available from: https://doi.org/10.1097/MD.0000000000012164
Lin Y, Shi R, XW, Shen H. Luteolin, a flavonoid with potentials for cancer prevention and Therapy. Current Cancer Drug Targets. 2008;8(7):634–646. Available from: https://doi.org/10.2174/156800908786241050
Park S, Ham S, Kwon TH, Kim MS, Lee DH, Kang J, et al. Luteolin Induces Cell Cycle Arrest and Apoptosis Through Extrinsic and Intrinsic Signaling Pathways in MCF-7 Breast Cancer Cells. Journal of Environmental Pathology Toxicology and Oncology. 2014;33(3):219–231. Available from: https://doi.org/10.1615/JEnvironPatholToxicolOncol.2014010923
Brusselmans K, Vrolix R, Verhoeven G, Swinnen JV. Induction of Cancer Cell Apoptosis by Flavonoids Is Associated with Their Ability to Inhibit Fatty Acid Synthase Activity. Journal of Biological Chemistry. 2005;280(7):5636–5645. Available from: https://dx.doi.org/10.1074/jbc.m408177200
Cook MT. Mechanism of Metastasis Suppression by Luteolin in Breast Cancer. Breast Cancer. 2012;10:89–100. Available from: https://doi.org/10.2147/BCTT.S144202
Alghasham AA. Cucurbitacins : A Promising Target for Cancer Therapy. International Journal of Health Sciences. 2013;7(1):77–89. Available from: https://dx.doi.org/10.12816/0006025

Copyright

© 2020 Sathya et al.This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee).