Czech J. Food Sci., 2013, 31(1):20-26 | DOI: 10.17221/9/2012-CJFS

Effects of season and time of milking on spontaneous and induced lipolysis in bovine milk fatOriginal Paper

Renáta TOUŠOVÁ, Luděk STÁDNÍK, Jaromír DUCHÁČEK
Department of Animal Husbandry, Faculty of Agrobiology, Food and Natural Sources, Czech University of Life Sciences Prague, Prague-Suchdol, Czech Republic

The effects were evaluated of different factors on the level of spontaneous (SPO) and induced (IND) lipolysis as defined by the content of free fatty acids (FFA) in milk. Milk samples were collected at monthly intervals throughout the year from both morning and evening milkings either individually in a milking parlour (SPO; n = 10) or from the bulk tank (IND; n = 10). The data were analysed using SAS 9.1. More intensive SPO was observed from March to May with higher FFA contents (+0.034 to +0.523 mmol/100 g of fat; P < 0.05-0.01), and also from September to November (+0.077 to +0.292 mmol/100 g of fat; P < 0.05). More intensive SPO was also detected in the evening milk than in that coming from morning milking (+0.062 to +0.556 mmol/100 g of fat; P < 0.05-0.01). SPO measured immediately after milking was affected by the season and time of milking. The content of FFA characterising IND in bulk milk (0.33-1.10 mmol/100 g of fat) was higher (P < 0.05-0.001) than that due to SPO in individual samples (0.21-0.86 mmol/100 g of fat), especially in those from evening milking compared to morning milking (+0.10 to +0.47 vs. +0.12 to +0.22 mmol/100 g of fat; P < 0.05-0.001).

Keywords: dairy cow; free fatty acid; lipolysis; milking; processing

Published: February 28, 2013  Show citation

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TOUŠOVÁ R, STÁDNÍK L, DUCHÁČEK J. Effects of season and time of milking on spontaneous and induced lipolysis in bovine milk fat. Czech J. Food Sci.. 2013;31(1):20-26. doi: 10.17221/9/2012-CJFS.
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References

  1. Abeni F., Degano L., Calza F., Giangiacomo R., Pirlo G. (2005): Milk quality and automatic milking: Fat globule size, natural creaming, and lipolysis. Journal of Dairy Science, 88: 3519-3529. Go to original source... Go to PubMed...
  2. Cempírková R., Mikulová M. (2009): Incidence of psychrotrophic lipolytic bakteria in cow's raw milk. Czech Journal of Animal Science, 54: 65-73. Go to original source...
  3. Cieslak A., Kowalczyk J. Czauderna M., Potkanski A., Szumacher-Strabel M. (2010): Enhancing unsaturated fatty acids in ewe's milk by feeding rapeseed or linseed oil. Czech Journal of Animal Science, 55: 496-504. Go to original source...
  4. Dai X.J., Wang C., Zhu Q. (2011): Milk performance of dairy cows supplemented with rapeseed oil, peanut oil and sunflower seed oil. Czech Journal of Animal Science, 56: 181-191. Go to original source...
  5. Deeth H.C. (2006): Lipoprotein lipase and lipolysis in milk. International Dairy Journal, 16: 555-562. Go to original source...
  6. Deeth H.C., Fitz-Gerald C.H. (1995): Lipolytic enzymes and hydrolytic rancidity in milk and dairy products. In: Fox P.F. (ed.): Advanced Dairy Chemistry. Vol. 2. Lipids. 2nd Ed. Chapman & Hall Publishers, London: 247-308.
  7. Ducháček J., Vacek M., Stádník L., Beran J., Okrouhlá M. (2012): Changes in milk fatty acid composition in relation to indicators of energy balance in Holstein cows. Acta Universitatis Agriculturae et Silviculturae Mendeleianae Brunensis, 60: 29-38. Go to original source...
  8. Evers J.M. (2004): The milkfat globule membrans - Methodologies for measuring milkfat globule (membrans) damage. International Dairy Journal, 14: 747-760. Go to original source...
  9. Evers J.M., Palfteyman K.R. (2001): Free fatty acid levels in New Zealand raw milk. Australian Journal of Dairy Technology, 56: 198-201.
  10. Forman L. (1984): Lipolýza mléčného tuku (I. část). Mlékařské listy: 310-313
  11. Frelich J., Šlachta M., Hanuš O., Špička J., Samková E. (2009): Fatty acid composition of cow milk fat produced on low-input mountain farms. Czech Journal of Animal Science, 54: 532-539. Go to original source...
  12. González-Martín I., Hernández-Hierro J.M., Revilla I., Vivar-Quintana A., Morón-Sancho R., SalvadorEsteban J. (2009): A comparative study of the analysis of free fatty acids in cheeses (cow's, ewe's and goat's) with different ripening times using nirs: intact samples and fat extracts. Czech Journal of Food Sciences, 27 (Special Issue 1): S114-D118. Go to original source...
  13. González F.D., Muiño R., Pereira V., Campos R., Benedito J.L. (2011): Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high-yielding dairy cows. Journal of Veterinary Science, 12: 251-255. Go to original source... Go to PubMed...
  14. Hanuš O., Janů L., Vyletělová M., Macek A., Zajíčková I., Kopecký J., Jedelská R., Nejeschlebová L. (2004): Vliv faktorů prvovýroby jako genotypu dojnice, krmení a bakteriální a mykotoxinové kontaminace mléka na jeho technologické ukazatele typu obsahu volných mastných kyselin, kysací schopnosti a syřitelnosti. In: Sborník příspěvků Aktuální problémy řízení v chovu skotu, Rapotín: 32-54.
  15. Hanuš O., Vegricht J., Frelich J., Macek A., Bjelka M., Louda F., Janů L. (2008): Analysis of raw cow milk quality according to free fatty acid contents in Czech Republic. Czech Journal of Animal Science, 53: 17-30. Go to original source...
  16. Hanuš O., Frelich J., Tomáška M., Vyletělová M., Genčurová V., Kučera J., Třináctý J. (2010): The analysis of relationships between chemical composition, physical, technological and health indicators and freezing point in raw cow milk. Czech Journal of Animal Science, 55: 11-29. Go to original source...
  17. Herdt T. (2007): Postabsorptive nutriet utilization. In: Cunningham J.G., Klein B.G. (eds): Veterinary Physiology. 4th Ed. Saunders/Elsevier, St. Louis: 389-407.
  18. IDF (1987): Significance of lipolysis in the manufacture and storage of dairy products. IDF Bulletin, No. 265. International Dairy Federation, Brussel.
  19. Kalač P., Samková E. (2010): The effects of feeding various forages on fatty acid composition of bovine milk fat: A review. Czech Journal of Animal Science, 55: 521-537. Go to original source...
  20. Kon H., Saito Z. (1997): Factors causing temperature activation of lipolysis in cow s milk. Milchwissenschaft, 52: 435-440.
  21. Kulig H., Kowalewska-Łuczak I., Kmieć M., WojdakMaksymiec K. (2010): ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traits in Jersey cows. Czech Journal of Animal Science, 55: 463-467. Go to original source...
  22. Miller B., Puhan Z. (1985): Das "lipolysierbare"Fett als Indikator für die Fettschädigung in der Rohmilk. Schweiz Milchwissenschaft Forschung, 15: 10-17.
  23. Navrátilová P., Borkovcová I., Vyhnálková J., Vorlové L. (2011): Fluoroquinolone residues in raw cow's milk. Czech Journal of Food Sciences, 29: 641-646. Go to original source...
  24. Nelson D.L., Cox M.M. (2000): Integration and hormonal regulativ of mammalian metabolism. In: Nelson D.L., Cox M.M. (eds): Lehninger Principles of Biochemistry. 3rd Ed. Worth Publishers, New York: 869-890.
  25. Poláková K., Kudrna V., Kodeš A., Hučko B., Mudřík Z. (2010): Non-structural carbohydrates in the nutrition of high-yielding dairy cows during a transition period. Czech Journal of Animal Science, 55: 468-478. Go to original source...
  26. Quattar G.C., Jeon I.J., Hart-Thakur R.A., Schmidt K.A. (2004): Fatty acids released from milk fat by lipoprotein lipase and lipolytic psychotrophs. Journal of Food Science C: Food Chemistry and Toxicology, 68: 659-664. Go to original source...
  27. Samková E., Pešek M., Špička J., Pelikánová T., Hanuš O. (2009): The effect of feeding diets markety differing in the proportion of grass and maize silages on bovine milk fat composition. Czech Journal of Animal Science, 54: 93-100. Go to original source...
  28. SAS (2004): SAS/STAT® 9.1. User's Guide. SAS Institute Inc., Cary.
  29. Sojková K., Hanuš O., Říha J., Genčurová V., Hulová I., Jedelská R., Kopecký J. (2010): Impacts of lactation physiology at higher and average yield on composition, properties and health indicators of milk in holstein breed. Scientia Agriculturae Bohemica, 41: 21-28.
  30. Wilking L., Nielsen J.H., Bavius A.K., Edvardsson A., Svennersten-Sjaunja K. (2006): Impact of milking frequencies on the level of free fatty acids in milk, fat globule size, and fatty acid composition. Journal of Dairy Science, 89: 1004-1009. Go to original source... Go to PubMed...

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