Czech J. Food Sci., 2016, 34(6):541-546 | DOI: 10.17221/72/2016-CJFS

Immobilisation of endoinulinase on polyhydroxybutyrate microfibersFood Technology and Economy, Engineering and Physical Properties

Milos Beran, Jitka Pinkrová, Marian Urban, Josef Drahorád
Department of Quality Features and Microbial Products, Food Research Institute Prague, Prague, Czech Republic

Due to the health benefits associated with the consumption of prebiotic short-chain fructooligosaccharides (sc-FOS) and inulooligosaccharides (IOS), there is increased interest in the use of these compounds in food products. We have developed a new biocatalyst for the production of FOS and IOS by inulin hydrolysis. Endoinulinase from Aspergillus niger Inulinase® Novozym 960 (Novozymes) was immobilised on polyhydroxybutyrate (PHB) nanofibres and microfibres by hydrophobic interactions. The PHB fibres were prepared by centrifugal spinning. FOS and IOS profiles were determined by ion-exchange chromatography with the Rezex RSO-Oligosaccharide column. The biocatalyst had very good activity and stability after repeated applications. It can be used in biocatalytic membrane reactors for the production of prebiotic oligosaccharides.

Keywords: inulin; fructooligosaccharide; inulooligosaccharide; inulinase; nanofibres; PHB

Published: December 31, 2016  Show citation

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Beran M, Pinkrová J, Urban M, Drahorád J. Immobilisation of endoinulinase on polyhydroxybutyrate microfibers. Czech J. Food Sci.. 2016;34(6):541-546. doi: 10.17221/72/2016-CJFS.
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