J. For. Sci., 2015, 61(4):182-187 | DOI: 10.17221/63/2014-JFS

Cryopreservation of Pistacia vera embryonic axesShort Communication

B. Benmahioul1, F. Daguin2, M. Kaïd-Harche3
1 Department of Forest Resources, Faculty of Natural Sciences and Life, Earth Sciences and the Universe, University of Tlemcen, Algeria
2 UMR 1345 Research Institute of Horticulture and Seeds, University of Angers, France Angers, France
3 Department of Biotechnology, Faculty of Sciences, University of Sciences and Technology of Oran Mohamed Boudiaf, Algeria

This preliminary study investigated the conservation of Pistacia vera genetic resources using seeds and isolated embryonic axes. First, the effect of storing seeds in ambient conditions on embryo viability was evaluated by in vitro culture. The germination rate of P. vera embryonic axes gradually decreased from 100% to 31% after 30-month storage of seeds. Cryopreservation may thus be necessary for the long-term conservation of embryos. A simple protocol was set up using embryonic axes. It included a single dehydration step with silica gel prior to direct freezing in liquid nitrogen (-196°C). The optimal germination rate was obtained after 60 min dehydration (water content of 0.2 grams of water per gram of dry weight [g.g-1 DW]). However, 90 minutes of dehydration (0.14 g.g-1 DW) were necessary to obtain seedlings whose qualitative development was equivalent to that of the control embryonic axes.

Keywords: seed; dehydration; water content; liquid nitrogen; germination

Published: April 30, 2015  Show citation

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Benmahioul B, Daguin F, Kaïd-Harche M. Cryopreservation of Pistacia vera embryonic axes. J. For. Sci.. 2015;61(4):182-187. doi: 10.17221/63/2014-JFS.
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