Czech J. Anim. Sci., 2017, 62(7):269-275 | DOI: 10.17221/58/2016-CJAS

Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter sizeOriginal Paper

Xiaopeng An1, Lijuan Bao1, Jinxing Hou1, Yueyu Bai2, Xinyan Zhao3, Yuxuan Song1, Binyun Cao*,1
1 College of Animal Science and Technology, Northwest A&F University, Yangling, P.R. China
2 Animal Health Supervision Institute of Henan Province, Zhengzhou, P.R. China
3 Northwest A&F University of Hospital, Northwest A&F University, Yangling, P.R. China

Gonadotropin-inhibitory hormone (GnIH) can decrease luteinizing hormone and/or follicle-stimulating hormone levels in rat, mouse, sheep, and cattle by the direct suppression of gonadotropin-releasing hormone (GnRH). The present study investigated polymorphisms in the GnIH genes of two dairy goat breeds and their association with litter size. Single nucleotide polymorphisms (SNPs) g.1837C>G and g.3195G>A (GenBank Accession Nos. KR778885 and KR819142) were detected in the GnIH genes of Xinong Saanen and Guanzhong dairy goat breeds using DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Furthermore, the g.1837C>G and g.3195G>A loci were closely linked in both breeds (r2 > 0.33). Association analysis showed that these SNPs had significant effects on the litter size of goats (P < 0.05). In both breeds, individuals with the CC/GG (g.1837C>G/g.3195G>A) genotype showed larger litter sizes in the second and average parities than individuals with the GG/AA genotype (P < 0.05). Known biochemical and physiological functions, along with our results, indicate that the CC/GG genotype may be used in marker-assisted selection to choose individuals with a larger litter size from both breeds.

Keywords: PCR-RFLP; SNPs; combined genotype; candidate gene

Published: July 31, 2017  Show citation

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An X, Bao L, Hou J, Bai Y, Zhao X, Song Y, Cao B. Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size. Czech J. Anim. Sci.. 2017;62(7):269-275. doi: 10.17221/58/2016-CJAS.
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