Plant Protect. Sci., 2023, 59(1):59-72 | DOI: 10.17221/48/2022-PPS

Characterisation of the HbSnRK2 gene family members and revealing specific HbSnRK2.2 functions in the stress resistance of the rubber treeOriginal Paper

Yu Zhang1, Mingyang Liu1,2, Dong Zhang1, Meng Wang1*, Lifeng Wang2*
1 Sanya Nanfan Research Institute, Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests; Ministry of Education of PRC; School of Plant Protection, Hainan University, Haikou, P.R. China
2 Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, Haikou, P.R. China; State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops, Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou, P.R. China; Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, P.R. China

SNF1-related protein kinase (SnRK2) is a critical positive regulatory factor in the abscisic acid (ABA) signalling pathway. However, the roles of the HbSnRK2 gene family members in the rubber tree, especially in response to stress, have not been thoroughly characterised. Here, we cloned six HbSnRK2 genes from the rubber tree. Based on the phylogenetic analysis, the HbSnRK2 family genes were divided into three groups. The motifs and intron numbers of HbSnRK2 were conserved. Analysis of cis-regulatory element sequences of all HbSnRK2 genes identified ABRE and TC-rich elements in the prompter of all the HbSnRK2 genes, illustrating that HbSnRK2 could be adjusted by the ABA and stress responsiveness. The qRT-PCR analysis showed that the expression patterns of the six HbSnRK2 genes differed in different tissues. The expression of these genes also differed under treatment with the plant hormone ABA, the HbSnRK2.2 gene was especially significantly expressed under the ABA treatment. Moreover, the HbSnRK2.2 gene responded to glyphosate, powdery mildew, heat stress and cold stress processes, which indicates that the HbSnRK2.2 gene plays an important role in phytohormone signalling and stress response in rubber trees. Taken together, the study provides valuable information to further define the role of the HbSnRK2 gene in rubber trees.

Keywords: SnRK2; Hevea brasiliensis Müll. Arg.; phytohormone; signal pathway

Received: April 18, 2022; Accepted: October 5, 2022; Prepublished online: January 25, 2023; Published: January 31, 2023  Show citation

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Zhang Y, Liu M, Zhang D, Wang M, Wang L. Characterisation of the HbSnRK2 gene family members and revealing specific HbSnRK2.2 functions in the stress resistance of the rubber tree. Plant Protect. Sci.. 2023;59(1):59-72. doi: 10.17221/48/2022-PPS.
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