Czech J. Anim. Sci., 2004, 49(5):177-182 | DOI: 10.17221/4297-CJAS

Analysis of amino acid composition of sheep colostrum by near-infrared spectroscopy

S. Kráčmar, R. Jankovská, K. ©ustová, J. Kuchtík, L. Zeman
Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno, Czech Republic

This paper deals with changes in the basic composition of sheep colostrum within the first 72 hours after parturition on the one hand and with the possibility of determining the major components of sheep colostrum by near-infrared spectroscopy on the other. Levels of essential, nonessential and total amino acids in sheep colostrum were determined by near-infrared reflectance spectroscopy (NIRS). ). For each component, sets of 90 samples were used to calibrate the instrument by means of a modified partial least-squares regression. The values of correlation coefficients (r) were as follows: 0.979 for Thr; 0.954 for Val; 0.968 for Leu; 0.918 for Ile; 0.946 for Lys; 0.908 for Arg; 0.845 for His; 0.999 for Trp; 0.915 for Phe; 0.909 for Met; 0.939 for Cys; 0.911 for Σmet + Cys; 0.933 for Tyr; 0.945 for Asp; 0.935 for Glu; 0.986 for Ser; 0.985 for Pro; 0.957 for Gly; 0.949 for Ala; 0.940 for ΣEAA; 0.958 for ΣNEAA and 0.977 for ΣAA. Partial least-squares (PLS) regression was used to develop calibration models for examined samples of sheep colostrum. When using the NIRS method, the following correlation coefficients were calculated: Thr (0.959), Val (0.912), Leu (0.936), Ile (0.855), Lys (0.903), Arg (0.853), His (0.717), Trp (0.667), Phe (0.854), Met (0.867), Cys (0.895), Σmet + Cys (0.868), Tyr (0.886), Asp (0.910), Glu (0.882), Ser (0.968), Pro (0.968), Gly (0.923), Ala (0.916), ΣEAA (0.901), ΣNEAA (0.923) and ΣAA (0.943). Calibration was tested using the same set of samples.NIRS results were compared with reference data and no significant differences between them were found (P = 0.05). Calibration and validation models were constructed in the same way.Results of this study indicate that NIR spectroscopy can be used for a rapid analysis of amino acid contents in sheep colostrum.

Keywords: near-infrared spectroscopy; sheep colostrum; amino acids

Published: May 31, 2004  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Kráčmar S, Jankovská R, ©ustová K, Kuchtík J, Zeman L. Analysis of amino acid composition of sheep colostrum by near-infrared spectroscopy. Czech J. Anim. Sci.. 2004;49(5):177-182. doi: 10.17221/4297-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Gajdůąek S., Kráčmar S., Kuchtík J., Jelínek P. (2003): Changes in basic composition of sheep colostrums (during first 72 hours a er parturition). Acta Univ. Agric. Silvic. Mendel. Brun., 51, 55-60.
    2. Jankovská R., ©ustová K. (2003): Analysis of cow milk by near-infrared spectroscopy. Czech J. Food Sci., 21, 123-128. Go to original source...
    3. Kráčmar S., Gajdůąek S., Jelínek P., Zeman L., Kozel V., Kozlová M., Kráčmarová E. (1999): Changes in amino acid composition of goat's colostrum during the first 72 hours a er birth. Czech J. Anim. Sci., 44, 541-545.
    4. Kráčmar S., Liąka I. (2002): Nebojme se stanovení tryptofanu. Krmivářství, 6, 40.
    5. Kráčmar S., Kuchtík J., Baran M., Váradyová Z., Kráčmarová E., Gajdůąek S., Jelínek P. (2004): Dynamics of changes in contents of organic and inorganic substances in sheep colostrums within the first 72 hours a er parturition. (Prepared to for publication). Go to original source...
    6. Kukačková O., Čurda L., Jindřich J. (2000): Multivariate calibration of raw cow milk using NIR spectroscopy. Czech J. Food Sci., 18, 1-4.
    7. Official Journal L 206 (1978): Eighth Commission Directive 78/633/EEC of 15 June 1978 Establishing Community Methods of Analysis for the Official Control of Feedingstuffs. July 29, 0043-0055.
    8. Rodriguez-Otero J.L., Hermida M., Centeno J. (1997): Analysis of Dairy Products by Near-Infrared Spectroscopy: A Rewiew. J. Agric. Food Chem., 45, 2815-2818. Go to original source...
    9. Ru Y.J., Glatz P.C. (2000): Application of Near Infrared Spectroscopy (NIR) for Monitoring the Quality of Milk, Cheese, Meat and Fish. Asian-Aus. J. Anim. Sci., 13, 1017-1025. Go to original source...
    10. Sato T., Yoshino M., Farukawa S., Someya Y., Yano N., Uozumi J., Iwamoto M. (1987): Analysis of milk constituents by the near infrared spectrophotometer method. Jpn. J. Zootech. Sci., 58, 698-706. Go to original source...
    11. Snedecor G.W., Cochran W.G. (1967): Statistical Methods. 6th ed. Iowa State, University Press, Iowa. 579 pp.
    12. ©ustová K., Jankovská R., Kráčmar S. (2004): Analysis of basic composition of sheep colostrums by near-infrared spectroscopy. (Prepared to for publication).
    13. Tsenkova R., Atanassova S., Toyoda K., Ozaki Y., Itoh K., Fearn T. (1999): Near-Infrared spectroscopy for dairy management: measurement of unhomogenized milk composition. J. Dairy Sci., 82, 2344-2351. Go to original source... Go to PubMed...
    14. Tsenkova R., Atanassova S., Itoh K., Ozaki Y., Toyoda K. (2000): Near infrared spectroscopy for bio monitoring: Cow milk composition measurement in a spectral region from 1,100 to 2,400 nanometers. Am. Sci. Anim., 78, 515-522. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content