Czech J. Genet. Plant Breed., 2003, 39(2):51-57 | DOI: 10.17221/3719-CJGPB
Identification of Glutenin Markers in Cultivars of three Wheat Species
- Department of Biochemistry and Biotechnology, Faculty of Biotechnology and Food Production, Slovak Agricultural University in Nitra, Nitra, Slovak Republic
In a collection of 41 Slovak and European winter wheat cultivars (Triticum aestivum L.), 5 spelt wheat cultivars (Triticum spelta L.) and 3 durum wheat cultivars (Triticum durum DESF.) we investigated the qualitative composition of high-molecular-weight glutenin subunits (HMW-GS), the Gluten quality (GQ) score and the presence of the secale block 1B3. The highest frequency of the HMW-GS 0, 7 + 9 and 5 + 10 was found among the winter wheat cultivars. The highest GQ score of 10 was found in the cultivars SK-30 and FD-92017/1. A GQ score of 9 was found in the cultivars Astella, Ilona, Velta and MV-06-95. The HMW-GS 0 and 7 + 8, associated with a GQ of 4, were found in T. durum. The electrophoretic spectra of the spelt wheat cultivars were not homogeneous and represented 4 different lines.
Keywords: Triticum aestivum L.; Triticum spelta L.; Triticum durum DESF.; HMW glutenin subunits; secale block 1B3; electrophoresis; SDS-PAGE; A-PAGE
Published: June 30, 2003 Show citation
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