Vet Med - Czech, 2022, 67(10):510-518 | DOI: 10.17221/36/2022-VETMED

Effect of the vaccination against Shiga toxin 2e in a farm with history of oedema disease, caused by atypical Escherichia coli producing Shiga toxin (STEC)Original Paper

D Sperling1, N Isaka1, H Karembe1, J Vanhara1,4, J Vinduska2, N Strakova3, A Kalova3,5, IvKolackova3, R Karpiskova3
1 Ceva Sante Animale France, Libourne, France
2 ZOD Zichlinek, Zichlinek, Czech Republic
3 Veterinary Research Institute, Brno, Czech Republic
4 Swine and Ruminant Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic
5 Department of Experimental Biology, Faculty of Science, Masaryk University Brno, Brno, Czech Republic

Oedema disease of weaned piglets is caused by shigatoxigenic Escherichia coli (STEC), typically harbouring the stx2e gene and F18 adhesins. The aim of this study was to assess the effect of a commercially available oedema disease vaccine on the zootechnical performance, mortality and individual antibiotic treatment in a herd, in which non-typical STEC strains without F18 adhesin have been identified. The zootechnical performance (average daily gain, total weight gain), mortality and individual antibiotic treatment were compared between vaccinated and non-vaccinated control piglets in a monocentric field efficacy study, which was performed using two groups in a parallel, randomised design. A significantly higher average daily gain and total weight gain were recorded in the vaccinated piglets in comparison to the controls. The lower morbidity, mortality and antibiotic treatment in piglets in the vaccine group were not statistically significant. As a conclusion, the positive effect of the vaccination was confirmed in the herd with prevalent STEC not harbouring F18 adhesin. The vaccine was, therefore, also effective against oedema disease caused by such unusual STEC isolates, under the conditions of this study.

Keywords: adhesins; pig; STEC; toxoid; whole-genome sequencing (WGS)

Received: April 13, 2022; Accepted: July 13, 2022; Prepublished online: September 1, 2022; Published: October 15, 2022  Show citation

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Sperling D, Isaka N, Karembe H, Vanhara J, Vinduska J, Strakova N, et al.. Effect of the vaccination against Shiga toxin 2e in a farm with history of oedema disease, caused by atypical Escherichia coli producing Shiga toxin (STEC). Vet Med-Czech. 2022;67(10):510-518. doi: 10.17221/36/2022-VETMED.
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    References

    1. Alexa P, Stouracova K, Hamrik J, Salajka E. Gene typing of the colonisation factors F18 of Escherichia coli isolated from piglets suffering from post-weaning oedema disease. Vet Med-Czech. 2002 May;47(5):132-6. Go to original source...
    2. Autret D, Leneveu P, Jardin A, Pelleau H, Goues T, Perrot O. ECOPORC SHIGA vaccination and performance improvement. In: Proceedings of the European Symposium of Porcine Health Management (ESPHM); 9-11 May 2018; Barcelona, Spain; 2018. p. 290.
    3. Baranzoni GM, Fratamico PM, Gangiredla J, Patel I, Bagi LK, Delannoy S, Fach P, Boccia F, Anastasio A, Pepe T. Characterization of Shiga toxin subtypes and virulence genes in porcine Shiga toxin-producing Escherichia coli. Front Microbiol. 2016 Apr 21;7:574. Go to original source... Go to PubMed...
    4. Bastert O, Fricke R, Sudendey C, Gnielka D, Luder O. Effect of vaccination with ECOPORC SHIGA on overall mortality and use of antimicrobial medication due to edema disease (ED). In: Proceedings of the 5th European Symposium of Porcine Health Management (ESPHM); 22-24 May 2013; United Kingdom, Edinburgh; 2013. p. 202.
    5. Creach P, Lemey R, Willems L. Economic impacts of the vaccination with ECOPORC SHIGA in a closed herd farm. In: Proceedings of the 7th European Symposium of Porcine Health Management (ESPHM); 22-24 Apr 2015; Nantes, France, Brussels; 2015. p. 208.
    6. Fricke R, Bastert O, Gotter V, Brons N, Kamp J, Selbitz HJ. Implementation of a vaccine against Shigatoxin 2e in a piglet producing farm with problems of oedema disease: Case study. Porcine Health Manag. 2015 May 7; 1(1): [5]. Go to original source... Go to PubMed...
    7. Gentry MK, Dalrymple JM. Quantitative microtiter cytotoxicity assay for Shigella toxin. J Clin Microbiol. 1980 Sep;12(3):361-6. Go to original source... Go to PubMed...
    8. Hahn E, Wild P, Schraner EM, Bertschinger HU, Haner M, Muller SA, Aebi U. Structural analysis of F18 fimbriae expressed by porcine toxigenic Escherichia coli. J Struct Biol. 2000 Dec;132(3):241-50. Go to original source... Go to PubMed...
    9. Hornich M, Salajka E, Sarmanova Z, Ulmann L. Enteric Escherichia coli infections in piglets. Morphological findings and localization of the causative agent by immunofluorescence. Acta Vet. 1973;42:413-22.
    10. Imberechts H, De Greve H, Schlicker C, Bouchet H, Pohl P, Charlier G, Bertschinger H, Wild P, Vandekerckhove J, Van Damme J, et al. Characterization of F107 fimbriae of Escherichia coli 107/86, which causes edema disease in pigs, and nucleotide sequence of the F107 major fimbrial subunit gene, fedA. Infect Immun. 1992 May;60(5): 1963-71. Go to original source... Go to PubMed...
    11. Imberechts H, Bertschinger HU, Stamm M, Sydler T, Pohl P, De Greve H, Hernalsteens JP, Van Montagu M, Lintermans P. Prevalence of F107 fimbriae on Escherichia coli isolated from pigs with oedema disease or postweaning diarrhoea. Vet Microbiol. 1994 Jun;40(3-4):219-30. Go to original source... Go to PubMed...
    12. Joensen KG, Tetzschner AM, Iguchi A, Aarestrup FM, Scheutz F. Rapid and easy in silico serotyping of Escherichia coli isolates by use of whole-genome sequencing data. J Clin Microbiol. 2015 Aug;53(8):2410-26. Go to original source... Go to PubMed...
    13. Johansen M, Fricke R, Bastert O, Nielsen MBR, Svensmark B, Haugegaard S, Baekbo P. Successful reduction of mortality by vaccination against oedema disease. In: Proceedings of the 5th European Symposium of Porcine Health Management; 22-24 May 2013; Edinburgh, United Kingdom; 2013. p. 195.
    14. Kausche FM, Dean EA, Arp LH, Samuel JE, Moon HW. An experimental model for subclinical edema disease (Escherichia coli enterotoxemia) manifest as vascular necrosis in pigs. Am J Vet Res. 1992 Mar;53(3):281-7.
    15. Leneveu P, Bourguignon P, Jardin A, Diesing AK, Creach P, Gotter V. Importance of single animal vaccination with a toxoid vaccine against edema disease. In: Proceedings of 9th European Symposium of Porcine Health Management (ESPHM); 3-5 May 2017; Prague, Czech Republic; 2017. p. 202.
    16. Lillie-Jaschniski K, Kochling M, Lindner T. Erste Erfahrungen mit dem Einsatz von ECOPORC SHIGA, der neuen Vakzine gegen die Odemkrankheit - Auswertungen aus Feldversuchen in Deutschland [First experiences with ECOPORC SHIGA, the new vaccine against edema disease - Results evaluated in field trials performed in Germany]. Tierärztl Umsch. 2013;68(9):377-88. German.
    17. Linggood MA, Thompson JM. Verotoxin production among porcine strains of Escherichia coli and its association with oedema disease. J Med Microbiol. 1987 Dec;24(4):359-62. Go to original source... Go to PubMed...
    18. MacLeod DL, Gyles CL, Wilcock BP. Reproduction of edema disease of swine with purified Shiga-like toxin-II variant. Vet Pathol. 1991 Jan;28(1):66-73. Go to original source... Go to PubMed...
    19. Nurk S, Bankevich A, Antipov D, Gurevich AA, Korobeynikov A, Lapidus A, Prjibelski AD, Pyshkin A, Sirotkin A, Sirotkin Y, Stepanauskas R, Clingenpeel SR, Woyke T, McLean JS, Lasken R, Tesler G, Alekseyev MA, Pevzner PA. Assembling single-cell genomes and mini-metagenomes from chimeric MDA products. J Comput Biol. 2013 Oct;20(10):714-37. Go to original source... Go to PubMed...
    20. Osek J. Prevalence of virulence factors of Escherichia coli strains isolated from diarrheic and healthy piglets after weaning. Vet Microbiol. 1999 Aug 31;68(3-4):209-17. Go to original source... Go to PubMed...
    21. Roer L, Tchesnokova V, Allesoe R, Muradova M, Chattopadhyay S, Ahrenfeldt J, Thomsen MCF, Lund O, Hansen F, Hammerum AM, Sokurenko E, Hasman H. Development of a web tool for Escherichia coli subtyping based on fimH alleles. J Clin Microbiol. 2017 Aug;55(8):2538-43. Go to original source... Go to PubMed...
    22. Salajka E, Salajkova Z, Alexa P, Hornich M. Colonization factor different from K88, K99, F41 and 987P in enterotoxigenic Escherichia coli strains isolated from postweaning diarrhoea in pigs. Vet Microbiol. 1992 Sep;32(2): 163-75. Go to original source... Go to PubMed...
    23. Scollo A, Mazzoni C. Edema disease vaccination in weaners improves feed conversion rate. In: Proceedings of: 9th European Symposium of Porcine Health Management (ESPHM); 3-5 May 2017; Prague, Czech Republic; 2017. p. 365.
    24. Sidler X, Mattei S, Fricke R, Schmid W, Bastert O, Luder O, Becker A, Schmid W. Results of a field trial for vaccine against oedema disease. In: Proceedings of the 5th European Symposium of Porcine Health Management; 22-24 May 2013; Edinburgh, United Kingdom; 2013. 87 p.
    25. Werneburg GT, Thanassi DG. Pili assembled by the chaperone/usher pathway in Escherichia coli and Salmonella. EcoSal Plus. 2018 Mar;8(1):1-56. Go to original source... Go to PubMed...
    26. Wittig W, Klie H, Gallien P, Lehmann S, Timm M, Tschape H. Prevalence of the fimbrial antigens F18 and K88 and of enterotoxins and verotoxins among Escherichia coli isolated from weaned pigs. Zentralbl Bakteriol. 1995 Nov;283(1):95-104. Go to original source... Go to PubMed...
    27. Zhang W, Zhao M, Ruesch L, Omot A, Francis D. Prevalence of virulence genes in Escherichia coli strains recently isolated from young pigs with diarrhea in the US. Vet Microbiol. 2007 Jul 20;123(1-3):145-52. Go to original source... Go to PubMed...

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