Czech J. Anim. Sci., 2008, 53(11):462-465 | DOI: 10.17221/344-CJAS

Evaluation of serum homocysteine and oxidative stress during lactation in ewes

G. Piccione, S. Casella, A. Assenza, F. Fazio, G. Caola
Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, University of Messina, Messina, Italy

The aim of the present work was to measure and evaluate the effect of the stage of lactation on serum homocysteine and oxidative stress in ewes. For our study ten Comisana ewes, 3 years old, clinically healthy, with the same level of production and during the same milking period were used and antioxidant barrier (Oxy-adsorbent), reactive oxygen species (dROMs), thiol antioxidant barrier (SHp) and homocysteine (sHcy) were investigated. One-way repeated measures analysis of variance (ANOVA), followed by Bonferroni's test, showed statistical differences (P < 0.05 was considered statistically significant). The statistically significant variations of dROMs, Oxy-adsorbent, SHp and sHcy during the experimental period concerned suggest an increase of oxidative processes at the end of lactation which are indicative of a response to this stress. The systematic analysis of oxidative stress and its influence on homocysteine levels are a valid instrument for the assessment of the health status of an animal and of good management procedures in a period such as lactation in which the homeostatic processes of organisms are altered.

Keywords: antioxidant barrier; reactive oxygen species; thiol antioxidant barrier; homocysteine; lactation; sheep

Published: November 30, 2008  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Piccione G, Casella S, Assenza A, Fazio F, Caola G. Evaluation of serum homocysteine and oxidative stress during lactation in ewes. Czech J. Anim. Sci.. 2008;53(11):462-465. doi: 10.17221/344-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bertoni G., Maianti M.G., Cappa V. (1984): Changes in the lipidic and glucydic metabolism during the last period of pregnancy and early lactation in cows. In: Proceedings of the Italian Society of Buiatric, 16, 223-236.
    2. Chaudiere J., Ferrari-Iliou R. (1999): Intracellular antioxidants: from chemical to biochemical mechanisms. Food and Chemical Toxicology, 37, 949-962. Go to original source... Go to PubMed...
    3. Di Trana A., Celi P., Claps S., Fedele V., Rubino R. (2006): The effect of hot season and nutrition on the oxidative status and metabolic profile in dairy goats during mid lactation. Animal Science, 82, 717-722. Go to original source...
    4. Fetoui H., Garoui M., Zeghal N. (2008): Protein restriction in pregnant-and lactating rats-induced oxidative stress and hypohomocysteinaemia in their offspring. Journal of Animal Physiology and Animal Nutrition. (in press). Go to original source... Go to PubMed...
    5. Fridovich I. (1999): Fundamental aspects of reactive oxygen species, or what's the matter with oxygen? Annals of the New York Academy of Sciences, 893, 13-18. Go to original source... Go to PubMed...
    6. Kalantar-Zadeh K., Ikizler T.A., Block G., Avram M.M., Kopple J.D. (2003): Malnutrition-inflammation complex syndrome in dialysis patients: causes and consequences. American Journal of Kidney Diseases, 42, 864-881. Go to original source... Go to PubMed...
    7. Memisođullari R., Akcay F. (2004): Biochemical mechanisms in hyperhomocysteinemia. Türk Klinik Biyokimya Dergisi, 2, 41-49.
    8. Memisođullari R., Erdođmus B., Alp H.H., Bilgin C., Arbak P.M., Yavuz Ö. (2008): Serum homocysteine levels in highway toll collectors and the relationship with intima-media thickness of the carotid artery. Turkish Journal of Medical Sciences, 38, 1-5.
    9. McCord J.M. (2000): The evolution of free radicals and oxidative stress. The American Journal of Medicine, 108, 652-659. Go to original source... Go to PubMed...
    10. Moat S.J. (2008): Plasma total homocysteine: instigator or indicator of cardiovascular disease? Annals of Clinical Biochemistry, 45, 345-348. Go to original source... Go to PubMed...
    11. Panayiotidis M.I., Stabler S.P., Allen R.H., Ahmad A., White C.W. (2004): Cigarette smoke extract increases S-adenosilmethionine and cystathionine in human lung epithelial-like cells. Chemico-Biological Interactions, 147, 87-97. Go to original source... Go to PubMed...
    12. Piccione G., Borruso M., Fazio F., Grasso F., Caola G. (2006): Oxidative stress evaluation during milking period in the ewes. Journal of Applied Animal Research, 29, 109-112. Go to original source...
    13. Piccione G., Borruso M., Giannetto C., Morgante M., Giudice E. (2007): Assessment of oxidative stress in dry and lactating cows. Acta Agriculturae Scandinavica, Section A-Animal Science, 57, 101-104. Go to original source...
    14. Pfeiffer C.M., Huff D.L., Gunter E.W. (1999): Rapid and accurate HPLC assay for plasma total homocysteine and cysteine in a clinical laboratory setting. Clinical Chemistry, 45, 290-292. Go to original source...
    15. Trevisan M., Browne R., Ram M., Muti P., Freudenheim J., Carosella A.N., Armstrong D. (2001): Correlates of markers of oxidative status in the general population. American Journal of Epidemiology, 154, 348-356. Go to original source... Go to PubMed...
    16. Ueland P.M., Hustad S., Schneede J., Refsum H., Vollset S.E. (2001): Biological and clinical implications of the MTHFR C677T polymorphism. Trends in Pharmacological Science, 22, 195-201. Go to original source... Go to PubMed...
    17. Yeh Y.Y., Yeh S.M. (2006): Homocysteine-lowering action is another potential cardiovascular protective factor of aged garlic extract. The Journal of Nutrition, 136, 745-749. Go to original source... Go to PubMed...
    18. Zinellu A., Sotgia S., Caria M.A., Tangianu F., Casu G., Deiana L., Carru C. (2007): Effect of acute exercise on low molecular weight thiols in plasma. Scandinavian Journal of Medicine and Science in Sports, 17, 452-456. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content