Vet Med - Czech, 2010, 55(12):619-624 | DOI: 10.17221/2948-VETMED

Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats

I. Yaman, A.S. Durmus, S. Ceribasi, M. Yaman
Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey

This experiment was conducted in order to compare the effects of Nigella sativa (NS) and silver sulfadiazine (SSD) cream on healing of burn wounds in rats. Fifty four adult, male Wistar-albino rats were divided into three groups of equal numbers. A burn was generated on the backs of all the rats. The burned areas in the first, second and third groups were covered with daily cold cream (control), SSD cream and NS cream (50% NS oil + 50% cold cream), respectively. Four, nine, and 14 days later, the rats were sacrificed and the burned skin tissue samples were collected for histopathological examinations. Histopatological evaluations on the 4th, 9th and 14th days showed burn healing to be better in the NS and SSD groups with respect to the control group. Wound healing was significantly different among the groups at 4th, 9th and 14th days (P < 0.001). In conclusion, application of NS and SSD cream are effective in healing burn related skin wounds in the rat model.

Keywords: Nigella sativa; silver sulfadiazine; burn; rat

Published: December 31, 2010  Show citation

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Yaman I, Durmus AS, Ceribasi S, Yaman M. Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats. Vet Med-Czech. 2010;55(12):619-624. doi: 10.17221/2948-VETMED.
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    References

    1. Agarwal R, Kharya MD, Shrivastava R (1979): Antimicrobial and anthelmintic activities of the essential oil of Nigella sativa Linn. Indian Journal of Experimental Biology 17, 1264-1265.
    2. Akhtar AH, Ahmed KD, Gilani SN, Nazir A (1996): Antiulcer effects of aqueous extract of Nigella sativa and Pongamia pinnata in rats. Fitoterapia 3, 195-199.
    3. Aljabre SHM, Randhawa MA, Akhtar N, Alakloby OM, Alqurashi AM, Aldossary A (2005): Antidermatophyte activity of ether extract of Nigella sativa and its active principle, thymoquinone. Journal of Ethnopharmacology 101, 116-119. Go to original source... Go to PubMed...
    4. Arici M, Sagdic O, Gecgel U (2005): Antibacterial effect of Turkish black cumin (Nigella sativa L.) oils. Grasasy Aceites 56, 259-262. Go to original source...
    5. Burits M, Bucar F (2000): Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research 14, 323- 328. Go to original source...
    6. Daba MH, Abdel-Rahman MS (1998): Hepatoprotective activity of thymoquinone in isolated rat hepatocytes. Toxicology Letters 95, 23-29. Go to original source... Go to PubMed...
    7. Durmus AS, Han MC, Yaman I (2009): Comperative evaluation of collagenase and silver sulfadiazine on burned wound healing in rats. Firat Universitesi Saglik Bilimleri Veteriner Dergisi 23, 135-139.
    8. Eldad A, Weinberg A, Breiterman S, Chaouat M, Palanker D, Ben-Bassat H (1998): Early nonsurgical removal of chemically injured tissue enhances wound healing in partial thickness burns. Burns 24, 166-172. Go to original source... Go to PubMed...
    9. Gracia CG (2001): An open study comparing topical silver sulfadiazine and topical silver sulfadiazine-cerium nitrate in the treatment of moderate and severe burns. Burns 27, 67-74. Go to original source... Go to PubMed...
    10. Gregory RS, Piccolo N, Piccolo MT, Piccolo MS, Heggers JP (2002): Comparison of propolis skin cream to silver sulfadiazine: A naturopathic alternative to antibiotics in treatment of minor burns. Journal of Alternative and Complementary Medicine 8, 77-83. Go to original source... Go to PubMed...
    11. Han MC, Durmus AS, Karabulut E, Yaman I (2005): Effects of Turkish propolis and silver sulfadiazine on burn wound healing in rats. Revue de Medecine Veterinaire 156, 624-627.
    12. Hosnuter M, Gurel A, Babuccu O, Armutcu F, Kargi E, Isikdemir A (2004): The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury. Burns 30, 121-125. Go to original source... Go to PubMed...
    13. Hosseinzadeh H, Parvardeh S, Asl MN, Sadeghnia HR, Ziaee T (2007): Effect of thymoquinone and Nigella sativa seeds oil on lipid peroxidation level during global cerebral ischemia reperfusion injury in rat hippocampus. Phytomedicine 14, 621-627. Go to original source... Go to PubMed...
    14. Houghton PJ, Zarka R, de la Heras B, Hoult JRS (1995): Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Medica 61, 33-36. Go to original source... Go to PubMed...
    15. Jones FA (1996): Herbs-useful plants. Their role in history and today. European Journal of Gastroenterology and Hepatology 8, 1227-1231. Go to original source... Go to PubMed...
    16. Kanter M, Coskun O, Kalayci M, Buyukbas S, Cagavi F (2006): Neuroprotective effects of Nigella sativa on experimental spinal cord injury in rats. Human and Experimental Toxicology 25, 127-133. Go to original source... Go to PubMed...
    17. Keskin D, Karsan O, Ezirmik N, Ciftcioglu A (1999): The effects of alpha-tocopherol on fracture healing in rabbits (in Turkish). Turkish Journal of Arthroplasty 10, 207-210.
    18. Lautenbacher LM (1997): Schwarzkummelol. Deutsche Apotheker Zeitung 137, 68-69.
    19. Mansour MA, Ginawi OT, El-Hadiyah T, El-Khatib AS, Al-Shabanah OA, Al-Sawaf HA (2001): Effects of volatile oil constituents of Nigella sativa on carbon tetrachloride induced hepatotoxicity in mice: evidence for antioxidant effects of thymoquinone. Research Communications in Molecular Pathology and Pharmacology 110, 239-251. Go to PubMed...
    20. Mansour MA, Nagi MN, El-Khatib AS, Al-Bekairi AM (2002) Effects of thymoquinone on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase in different tissues of mice: a possible mechanism of action. Cell Biochemistry and Function 20, 143-151. Go to original source... Go to PubMed...
    21. Mashhadian NV, Rakhshandeh H (2005): Antibacterial and antifungal effects of Nigella sativa extracts against S. aureus, P. aeroginosa and C. albicans. Pakistan Journal of Medical Sciences 21, 47-52.
    22. Mekkes JR, Le Poole C, Das PK, Bos JD, Westerhof W (1998): Efficient debridement of necrotic wounds using proteolytic enzymes derived from Antarctic krill: a double-blind, placebocontrolled study in a standardized animal wound model. Wound Repair and Regeneration 6, 50-58. Go to original source... Go to PubMed...
    23. Meral I, Yener Z, Kahraman T, Mert N (2001): Effect of Nigella sativa on glucose concentration, lipid peroxidation, anti-oxidant defence system and liver damage in experimentally-induced diabetic rabbits. Journal of Veterinary Medicine Series A - Physiology Pathology Clinical Medicine 48, 593-599. Go to original source... Go to PubMed...
    24. Nayak S, Nalabothu P, Sandiford S, Bhogadi V, Adogwa A (2006): Evaluation of wound healing activity of Allamanda cathartica L. and Laurus nobilis. L. Extracts on rats. BMC Complementary and Alternative Medicine 6, 12. Go to original source... Go to PubMed...
    25. Phillips JD (1992): Medicinal plants. Biologist 39, 187- 191.
    26. Randhawa MA, Al-Ghamdi MS (2002): A review of the pharmacotherapeutic effects of Nigella sativa. Pakistan Journal of Medical Research 41, 77-83.
    27. Serarslan G, Altug ME, Kontas T (2007): The effects of cafeic acid phenethyl ester on plasma lipid peroxidation, antioxidant status and nitric oxide levels in incisional wound model (in Turkish). Turkderm Dergisi 41, 11-14.
    28. Varol Y (2008): Investigation of the effects of Nigella Sativa oil on cutaneous wound healing in rats. [Thesis]. T.C. Marmara Universitesi Saglik Bilimleri Enstitusu, Istanbul.
    29. Webster ME, Altieri PL, Conklin DA, Berman S, Lowenthal JP, Gochenour RB (1962): Enzymatic debridement of third-degree burns on guinea pigs by Clostridium histolyticum proteinases. Journal of Bacteriology 83, 602-608. Go to original source... Go to PubMed...
    30. Worthen DR, Ghosheh OA, Crooks PA (1998): The in vitro anti-tumor activity of some crude and purified components of blackseed, Nigella sativa L. Anticancer Research 18, 1527-1532.
    31. Yaman I, Balikci E (2010): Protective effects of nigella sativa against gentamicin-induced nephrotoxicity in rats. Experimental and Toxicologic Pathology 62, 183-190. Go to original source... Go to PubMed...

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