Czech J. Food Sci., 2013, 31(4):407-412 | DOI: 10.17221/286/2012-CJFS

Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheatOriginal Paper

Ta»ána Sumíková1, Ludmila Gabrielová2, Ladislav Kučera3, Martin ®abka2, Jana Chrpová1
1 Department of Genetics and Plant Breeding Methods,
2 Department of Mycology and
3 Department of Molecular Biology, Crop Research Institute, Prague, Czech Republic

Fusarium head blight (FHB) is a serious cereal disease in the CzechRepublic. The most important pathogen associated with FBH is Fusarium graminearum, which can produce trichothecenes, mainly deoxynivalenol (DON) and its derivates. A set of 103 F. graminearum isolates were isolated from naturally infected wheat ears collected from 20 localities (25 ears from one locality) within the Czech Republic, in the year 2004. The ears were evaluated for DON content by ELISA. Maximum detected value was 30.7 mg/kg. A group of PCR assays targeting the segments of the Tri7, Tri13 and Tri3 genes were used to determine the chemotypes of F. graminearum isolates. All the isolates belonged to DON producing chemotype. Further discrimination revealed that almost all (99.03%) isolates belonged to 15-ADON chemotype, and only one (0.97%) isolate belonged to 3-ADON chemotype. The genetic variability of the isolates was assessed from their AFLP fingerprints. The populations were highly heterogeneous both within and between locations, and no clear evidence for the association between AFLP profile and geographic origin was found out.

Keywords: food contaminant; electroanalysis; sample preparation; deoxynivalenol; AFLP; Triticum aestivum L.

Published: August 31, 2013  Show citation

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Sumíková T, Gabrielová L, Kučera L, ®abka M, Chrpová J. Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat. Czech J. Food Sci.. 2013;31(4):407-412. doi: 10.17221/286/2012-CJFS.
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References

  1. Audenaert K., Van Broeck R., Bekaert B., De Witte F., Heremans B., Messens K., Hoffe M., Haesaert G. (2009): Fusarium head blight (FHB) in Flanders: population diversity, inter-species associations and DON contamination in commercial winter wheat varieties. European Journal of Plant Pathology, 125: 445-458. Go to original source...
  2. Bowden R.L., Leslie J.F. (1999): Sexual recombination in Gibberella zeae. Phytopathology, 89: 182-188. Go to original source... Go to PubMed...
  3. Chandler E.A., Simpson D.R., Thomsett M.A., Nicholson P. (2003): Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes, and characterization of chemotypes of Fusarium graminearum, F. culmorum and F. cerealis. Physiological and Molecular Plant Pathology, 62: 355-367. Go to original source...
  4. Excoffier L., Laval G., Schneider S. (2005): Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics (online), 1: 47-50. Go to original source...
  5. Gale L.R., Chen L.-F., Hernick C.A., Takamura K., Kistler H.C. (2002): Population analysis of Fusarium graminearum from wheat fields in Eastern China. Phytopathology, 92: 315-1322. Go to original source... Go to PubMed...
  6. Gale L.R., Harrison S.A., Ward T.J., O'Donnell K., Milus E.A., Gale S.W., Kistler H.C. (2011): Nivalenol-type populations of Fusarium graminearum and F. asiaticum are prevalent on wheat in southern Louisiana. Phytopathology, 101: 124-134. Go to original source... Go to PubMed...
  7. Garvey G.S., McCormick S.P., Alexander N.J., Rayment I. (2009): Structural and functional characterization of Tri3 trichothecene 15-O-acetyltransferase from Fusarium sporotrichioides. Protein Science, 18: 747-761. Go to original source...
  8. Jennings P., Coates M.E., Turner J.A., Chandler E.A., Nicholson P. (2004): Determination of deoxynivalenon and nivalenon chemotypes of Fusarium culmorum isolates from England and Wales by PCR assays. Plant Pathology, 53: 182-190. Go to original source...
  9. Jestoi M. (2008): Emerging Fusarium-mycotoxins fusalproliferin, beauvericin, enniatins and moniliformin: a review. Critical Reviews in Food Science and Nutrition, 48: 21-49. Go to original source... Go to PubMed...
  10. Kammoun L.G., Gargouri S., Barreau C., RichardForget F., Hajlaoui M.R. (2010): Trichothecene chemotypes of Fusarium culmorum infecting wheat in Tunisia. International Journal of Food Microbiology, 140: 84-89. Go to original source... Go to PubMed...
  11. Kimura M., Tokai T., Takahashi-Ando N., Ohsato S., Fujimura M. (2007): Molecular and genetic studies of fusarium trichothecene biosynthesis: pathways, genes, and evolution. Bioscience, Biotechnology, and Biochemistry, 71: 2105-2123. Go to original source... Go to PubMed...
  12. Lee T., Oh D.W., Kim H.S., Lee J., Kim Y.H., Yun S.H., Lee Y.W. (2001): Identification of deoxynivalenol- and nivalenol-producing chemotypes of Giberella zeae by using PCR. Applied and Environmental Microbiology, 67: 2966-2972. Go to original source... Go to PubMed...
  13. Lee T., Han Y.K., Kim K.H., Yun S.H., Lee Y.W. (2002): Tri13 and Tri7 determine deoxynivalenol- and nivalenolproducing chemotypes of Gibberella zeae. Applied and Environmental Microbiology, 68: 2148-2154. Go to original source... Go to PubMed...
  14. Leslie J.F., Bowden R.L. (2008): Fusarium graminearum: when species concepts collide. Cereal Research Communication, 36: 609-615. Go to original source...
  15. Leslie J.F., Summerell B.A. (2006): The Fusarium Laboratory Manual. Blackwell Publishing, Ames. Go to original source...
  16. Maier F.J., Miedaner T., Hadeler B., Felk A., Salomon S., Lemmens M., Kassner H., Schafer W. (2006): Involvement of trichothecenes in fusarioses of wheat, barley and maize evaluated by gene disruption of the trichodiene synthase (Tri5) gene in three field isolates of different chemotype and virulence. Molecular Plant Pathology, 7: 449-451. Go to original source... Go to PubMed...
  17. Miedaner T., Cumagun C.J.R., Chakraborty S. (2008): Population genetics of three important head blight pathogens Fusarium graminearum, F. pseudograminearum and F. culmorum. Journal of Phytopathology, 156: 129-139. Go to original source...
  18. Naef A., Défago G. (2006): Population structure of plantpathogenic Fusarium species in overwintered stalk residues from Bt-transformed and non-transformed maize crops. European Journal of Plant Pathology, 116: 129-143. Go to original source...
  19. Nicholson P., Simpson D.R., Weston G., Rezanoor H.N., Lees A.K., Parry D.W., Joyce D. (1998): Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assay. Physiological and Molecular Plant Pathology, 53: 17-37. Go to original source...
  20. O'Donnell K., Ward T.J., Aberra D., Kistler H.C., Aoki T., Orwig N., Kimura M., Bjornstad A., Klemsdal S.S. (2008): Multilocus genotyping and molecular phylogenetics resolve a novel head blight pathogen within the Fusarium graminearum species complex from Ethiopia. Fungal Genetics and Biology, 45: 1514-1522. Go to original source... Go to PubMed...
  21. Perrier X., Jacquemoud-Collet J.P. (2006): DARwin software. Available at http://darwin.cirad.fr/darwin
  22. Poliąenská I., Sýkorová S., Matějová E., Chrpová J., Nedomová L. (2008): Occurrence of deoxynivalenol in Czech grains. World Mycotoxin Journal, 1: 299-305. Go to original source...
  23. Prodi A., Tonti S., Nipoti P., Pancaldi D., Pisi A. (2009): Identification of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolates from durum wheat in a restricted area of northern Italy. Journal of Plant Pathology, 91: 727-731.
  24. Qu B., Li H.P., Zhang J.B., Huang T., Carter J., Liao Y.C., Nicholson P. (2008): Comparison of genetic diversity and pathogenicity of Fusarium head blight pathogens from China and Europe by SSCP and seedling assays on wheat. Plant Pathology, 57: 642-651. Go to original source...
  25. Samson R.A., Hoekstra E., Frisvald J.C., Filtenborg O. (1996): Introduction to Food-borne Fungi. Centraal Bureau voor Schimmelcultures, Baarn.
  26. Sarver B.A.J., Ward T.J., Gale L.R., Broz K., Kistler C., Aoki T., Nicholson P., Carter J., O'Donnell K. (2011): Novel Fusarium head blight pathogens from Nepal and Louisiana revealed by multilocus genenalogical concordance. Fungal Genetics and Biology, 48: 1096-1107. Go to original source... Go to PubMed...
  27. Starkey D.E., Todd J.W., Aoki T., Liane R.G., Kistler H.C., Geiser D.M., Suga H., Toth B., Varga J., O'Donnell K. (2007): Global molecular surveillance reveals novel Fusarium head blight species and trichothecene toxin diversity. Fungal Genetics and Biology, 44: 1191-1204. Go to original source... Go to PubMed...
  28. Stepien L., Popiel D., Koczyk G., Chelkowski J. (2008): Wheat-infecting Fusarium species in Poland - their chemotypes and frequencies revealed by PCR assay. Journal of Applied Genetics, 49: 433-441. Go to original source... Go to PubMed...
  29. ©udyová V., ©liková S., Gregorová E. (2010): Natural occurrence of deoxynivalenol in kernels of wheat grown in Slovakia during 2004-2008. Journal of Food and Nutrition Research, 49: 89-93.
  30. Sumíková T., Remeąová J., Leiąová L., Kučera L., Chrpová J., ©íp V. (2010): AFLP Genotyping improves the level of discrimination between the Fusarium species responsible for head blight in wheat. Cereal Research Communications, 38: 533-540. Go to original source...
  31. Talas F., Parzies H.K., Miedaner T. (2011): Diversity in genetic structure and chemotype composition of Fusarium graminearum sensu stricto population causing head blight in individual fields in Germany. European Journal of Plant Pathology, 131: 39-48. Go to original source...
  32. Ward T.J., Bielawski J.P., Kistler H.C., Sullivan E., O´Donnell K. (2002): Ancestral polymorphism and adaptive evolution in the trichothecene mycotoxin gene cluster of phytophatogenic Fusarium. Proceedings of National Academy of Science USA, 99: 9278-9283. Go to original source... Go to PubMed...
  33. Yli-Mattila T., Gagkaeva T., Ward T.J., Aoki T., Kistler H.C., O'Donnell K. (2009): A novel Asian clade within Fusarium graminearum species complex includes a newly discovered cereal head blight pathogen from the Russian Far East. Mycologia, 101: 841-852. Go to original source... Go to PubMed...
  34. Zeller K.A, Browden R.L., Leslie J.F. (2004): Population differentiation and recombination in wheat scab populations of Gibberella zeae from United States. Molecular Ecology, 13: 563-571. Go to original source... Go to PubMed...

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