Czech J. Food Sci., 2017, 35(2):106-112 | DOI: 10.17221/260/2016-CJFS

Prevalence and characteristics of Salmonella in retail poultry and pork meat in the Czech Republic during 2013-2014Food Microbiology and Safety

Petra Myšková, Renáta Karpíšková*
Department of Bacteriology, Veterinary Research Institute, Brno, Czech Republic

The EN ISO 6579/2002 guideline was used for the detection of Salmonella in retail poultry and pork meat in the Czech Republic in 2013 and 2014. The laboratory confirmed isolates were further typed (slide agglutination, phage typing, resistance to antimicrobial agents, PCR for the detection of selected genes encoding plasmid mediated quinolone resistance). Out of 176 poultry and 223 pork meat samples, 24 (13.6%) and 6 (2.7%) were positive for the detection of Salmonella spp., respectively. In Salmonella isolates from poultry, 14 serotypes were differentiated with S. indiana, S. enteritidis and S. 6,7:-:1,5 being the most common serotypes. S. typhimurium and its monophasic variant S. 4,[5],12:i:- were predominant in pork meat. The overall resistance to one antimicrobial agent at least was high in both groups of isolates - 50% (poultry) and 71.4% (pork). No Salmonella isolate was confirmed to carry any of the selected PMQR genes. The study showed a higher prevalence of Salmonella in poultry, but pork meat also poses a risk to consumers.

Keywords: food; PCR; serotyping; phage typing; antimicrobial resistance

Published: April 30, 2017  Show citation

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Myšková P, Karpíšková R. Prevalence and characteristics of Salmonella in retail poultry and pork meat in the Czech Republic during 2013-2014. Czech J. Food Sci.. 2017;35(2):106-112. doi: 10.17221/260/2016-CJFS.
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    References

    1. Álvarez-Fernández E., Alonso-Calleja C., García-Fernández C., Capita R. (2012): Prevalence and antimicrobial resistance of Salmonella serotypes isolated from poultry in Spain: Comparison between 1993 and 2006. International Journal of Food Microbiology, 153: 281-287. Go to original source... Go to PubMed...
    2. Anderson E.S., Ward L.R., de Saxe M.J., de Sa J.D. (1977): Bacteriophage-typing designations of Salmonella typhimurium. The Journal of Hygiene, 78: 297-300. Go to original source... Go to PubMed...
    3. Anonymous (2002): Microbiology of food and animal feeding stuffs - Horizontal method for detection of Salmonella spp. European Committee for Standardization.
    4. Anonymous (2011): Commission Regulation No 1086/2011 of 27 October 2011 amending Annex II to Regulation (EC) No 2160/2003 of the European parliament and of the Council and Annex I to Commission Regulation (EC) No 2073/2005 as regards salmonella in fresh poultry meat. Official Journal of the European Union.
    5. Antunes P., Réu C., Sousa J.C., Peixe L., Pestana N. (2003): Incidence of Salmonella from poultry products and their susceptibility to antimicrobial agents. International Journal of Food Microbiology, 82: 97-103. Go to original source... Go to PubMed...
    6. Bone A., Noel H., Le Hello S., Pihier N., Danan C., Raguenaud M.E., Salah S., Bellali H., Vaillant V., Weill F.X., Jourdan-da Silva N. (2010): Nationwide outbreak of Salmonella enterica serotype 4,12:i:- infections in France, linked to dried pork sausage, March-May 2010. Eurosurveillance, 15: 19592. Go to original source...
    7. Carramiñana J.J., Rota C., Agustín I., Herrera A. (2004): High prevalence of multiple resistance to antibiotics in Salmonella serovars isolated from a poultry slaughterhouse in Spain. Veterinary Microbiology, 104: 133-139. Go to original source... Go to PubMed...
    8. Cattoir V., Poirel L., Rotimi V., Soussy C.J., Nordmann P. (2007): Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates. Journal of Antimicrobial Chemotherapy, 60: 394-397. Go to original source... Go to PubMed...
    9. Clinical and Laboratory Standard Institute (CLSI) (2012): Performance standards for antimicrobial susceptibility testing, Document M100-S22, USA.
    10. European Food Safety Authority (EFSA), European Centre for Disease Prevention and Control (2012): The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2010. EFSA Journal, 10: 2597. Go to original source...
    11. European Food Safety Authority (EFSA), European Centre for Disease Prevention and Control (2013): The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2011. EFSA Journal, 11: 3129. Go to original source...
    12. Fearnley E., Raupach J., Lagala F., Cameron S. (2011): Salmonella in chicken meat, eggs and humans; Adelaide, South Australia, 2008. International Journal of Food Microbiology, 146: 219-227. Go to original source... Go to PubMed...
    13. Glenn L.M., Lindsey R.L., Folster J.P., Pecic G., Boerlin P., Gilmour M.W., Harbottle H., Zhao S., McDermott P.F., Fedorka-Cray P.J., Frye J.G. (2013): Antimicrobial resistance genes in multidrug-resistant Salmonella enterica isolated from animals, retail meats, and humans in the United States and Canada. Microbial Drug Resistance, 19: 175-184. Go to original source... Go to PubMed...
    14. Gossner C.M., van Cauteren D., Le Hello S., Weill F.X., Terrien E., Tessier S., Janin C., Brisabois A., Dusch V., Vaillant V., Jourdan da Silva N. (2012): Nationwide outbreak of Salmonella enterica serotype 4,[5],12:i:- infection associated with consumption of dried pork sausage, France, November to December 2011. Eurosurveillance, 17: 20071. Go to original source... Go to PubMed...
    15. Grimont P., Weill F.X. (2007): Antigenic Formulae of the Salmonella Serovars. 9th Ed. Paris, WHO Collaborating Centre for Reference and Research on Salmonella: 166.
    16. Hopkins K.L., Kirchner M., Guerra B., Granier S.A., Lucarelli C., Porrero M.C., Jakubczak A., Threlfall E.J., Melvius D.J. (2010): Multiresistant Salmonella enterica serovar 4,[5],12:i:-in Europe: a new pandemic strain? Eurosurveillance, 15: 19580. Go to original source...
    17. Horiyama T., Nikaido E., Yamaguchi A., Nishino K. (2011): Roles of Salmonella multidrug efflux pumps in tigecycline resistance. Journal of Antimicrobial Chemotherapy, 66: 105-110. Go to original source... Go to PubMed...
    18. Kerouanton A., Rose V., Weill F.X., Granier S.A., Denis M. (2013): Genetic diversity and antimicrobial resistance profiles of Salmonella enterica serotype Derby isolated from pigs, pork and humans in France. Foodborne Pathogens and Disease, 10: 977-984. Go to original source... Go to PubMed...
    19. Kozak G.K., Pearl D.L., Parkman J., Reid-Smith R.J., Deckert A., Boerlin P. (2009): Distribution of sulfonamide resistance genes in Escherichia coli and Salmonella isolates from swine and chickens at abattoirs in Ontario and Québec, Canada. Applied Environmental Microbiology, 75: 5999-6001. Go to original source... Go to PubMed...
    20. Lukasz M., Mackiw E., Sciezynska M., Pawlowska K., Popowska M. (2014): Antimicrobial susceptibility of Salmonella strains isolated from retail meat products in Poland between 2008 and 2012. Food Control, 36: 199-204. Go to original source...
    21. Mohamed T., Zhao S., White D.G., Parveen S. (2014): Molecular characterization of antibiotic resistant Salmonella Typhimurium and Salmonella Kentucky isolated from pre- and post-chill whole broilers carcasses. Food Microbiology, 38: 6-15. Go to original source... Go to PubMed...
    22. Myšková P., Karpíšková R., Dědičová D. (2012): Výskyt monofazické varianty Salmonella 9,12:l,v:- v České republice. Veterinářství, 62: 632-634.
    23. Myšková P., Karpíšková R., Dědičová D. (2013): Epidemické případy salmonelóz v České republice v roce 2012. Epidemiologie, Mikrobiologie, Imunologie, 62: 59-63.
    24. Myskova P., Oslanecova L., Drahovska H., Karpiskova R. (2014): Clonal distribution of monophasic Salmonella enterica subsp. enterica serotype 4,[5],12:i:- in the Czech Republic. Foodborne Pathogens and Disease, 11: 664-666. Go to original source... Go to PubMed...
    25. Olsen J.E., Aabo S., Hill W., Notermans S., Wernars K., Granum P.E., Popovic T., Rasmussen H.N., Olsvik O. (1995): doi: 10.17221/260/2016-CJFS Probes and polymerase chain reaction for detection of food-borne bacterial pathogens. International Journal of Food Microbiology, 28: 1-78. Go to original source... Go to PubMed...
    26. Park C.H., Robicsek A., Jacoby G.A., Sahm D., Hooper D.C. (2006): Prevalence in the United States of aac(6)-Ib-cr encoding a ciprofloxacin-modifying enzyme. Antimicrobial Agents and Chemotherapy, 50: 3953-3955. Go to original source... Go to PubMed...
    27. Parry C.M., Threlafall E.J. (2008): Antimicrobial resistance in typhoidal and non-typhoidal salmonellae. Current Opinion in Infectious Diseases, 21: 531-538. Go to original source... Go to PubMed...
    28. Prendergast D.M., Duggan S.J., Gonzales-Barron U., Fanning S., Butler F., Cormican M., Duffy G. (2009): Prevalence, numbers and characteristics of Salmonella spp. on Irish retail pork. International Journal of Food Microbiology, 131: 233-239. Go to original source... Go to PubMed...
    29. Rahmani M., Peighambari S.M., Svendsen C.A., Cavaco L.M., Agersø Y., Hendriksen R.S. (2013): Molecular clonality and antimicrobial resistance in Salmonella enterica serovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Veterinary Research, 9: 66. Go to original source... Go to PubMed...
    30. Schwaiger K., Huther S., Hölzel C., Kämpf P., Bauer J. (2012): Prevalence of antibiotic-resistant Enterobacteriaceae isolated from chicken and pork meat purchased at the slaughterhouse and at retail in Bavaria, Germany. International Journal of Food Microbiology, 154: 206-211. Go to original source... Go to PubMed...
    31. Sechter I., Cahan D. (1984): Monophasic Salmonella 9,12:l,v:- identified as Salmonella goettingen. Israel Journal of Medical Sciences, 20: 472.
    32. Sisak F., Havlickova H., Hradecka H., Rychlik I., Kolackova I., Karpiskova R. (2006): Antibiotic resistance of Salmonella spp. isolates from pigs in the Czech Republic. Veterinární Medicína, 51: 303-310. Go to original source...
    33. Soomro A.H., Khaskheli M., Bhutto M.B., Shah G., Memon A., Dewani P. (2010): Prevalence and antimicrobial resistance of Salmonella serovars isolated from poultry meat in Hyderabad, Pakistan. Turkish Journal of Veterinary and Animal Sciences, 34: 455-460. Go to original source...
    34. Strahilevitz J., Jacoby G.A., Hooper D.C., Robicsek A. (2006): Plasmid-mediated quinolone resistance: a multifaced threat. Clinical Microbiology Reviews, 22: 664-689. Go to original source... Go to PubMed...
    35. Ta Y.T., Nguyen T.T., To P.B., Pham D.X., Le H.T.H., Thi G.N., Alali W.Q., Walls I., Doyle M.P. (2014): Quantification, serovars, and antibiotic resistance of Salmonella isolated from retail raw chicken meat in Vietnam. Journal of Food Protection, 1: 57-66. Go to original source... Go to PubMed...
    36. Thu Hao Van T., Moutafis G., Istivan T., Thuoc Tran L., Coloe P.J. (2007): Detection of Salmonella spp. in retail raw food samples from Vietnam and characterization of their antibiotic resistance. Applied Environmental Microbiology, 73: 6885-6892. Go to original source... Go to PubMed...
    37. Threlfall E.J. (2000): Epidemic Salmonella typhimurium DT 104 - a truly international multiresistant clone. Journal of Antimicrobial Chemotherapy, 46: 7-10. Go to original source... Go to PubMed...
    38. Verraes C., Van Boxstael S., Van Meervenne E., Van Coillie E., Butaye P., Catry B., de Schaetzen M.-A., Van Huffel A., Imberechts H., Dierick K., Daube G., Saegerman C., De Block J., Dewulf J., Herman L. (2013): Antimicrobial resistance in the food chain: a review. International Journal of Environmental Research and Public Health, 10: 2643-2669. Go to original source... Go to PubMed...
    39. Ward L.R., de Sa J.D.H., Rowe B. (1987): A phage-typing scheme for Salmonella enteritidis. Epidemiology and Infection, 1987: 291-294. Go to original source... Go to PubMed...
    40. Wasyl D., Hoszowski A., Zajac M. (2014): Prevalence and characterisation of quinolone resistance mechanisms in Salmonella spp. Veterinary Microbiology, 171: 307-314. Go to original source... Go to PubMed...
    41. White D.G., Zhao S., Sudler R., Ayers S., Friedman S., Chen S., McDermott P.F., McDermott S., Wagner D.D., Meng J. (2001): The isolation of antibiotic-resistant Salmonella from retail ground meats. New England Journal of Medicine, 345: 1147-1154. Go to original source... Go to PubMed...
    42. Wong M.H.Y., Zeng L., Liu J.H., Chen S. (2013): Characterization of Salmonella food isolates with concurrent resistance to ceftriaxone and ciprofloxacin. Foodborne Pathogens and Disease, 10: 42-46. Go to original source... Go to PubMed...
    43. Yamane K., Wachino J., Suzuki S., Arakawa Y. (2008): Plasmid-mediated qepA gene among Escherichia coli clinical isolates from Japan. Antimicrobial Agents and Chemotherapy, 52: 1564-1566. Go to original source... Go to PubMed...
    44. Yang B., Qu D., Zhang X., Shen J., Cui S., Shi Y., Xi M., Sheng M., Zhi S., Meng J. (2010): Prevalence and characterization of Salmonella serovars in retail meats of marketplace in Shaanxi, China. International Journal of Food Microbiology, 141: 63-72. Go to original source... Go to PubMed...
    45. Zdragas A., Mazaraki K., Vafeas G., Giantzi V., Papadopoulos T., Ekateriniadou L. (2012): Prevalence, seasonal occurrence and antimicrobial resistance of Salmonella in poultry retail products in Greece. Letters in Applied Microbiology, 55: 308-313. Go to original source... Go to PubMed...
    46. Zhao C., Ge B., De Villena J., Sudler R., Yeh E., Zhao S., White D.G., Wagner D., Meng J. (2001): Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the Greater Washington, D.C., Area. Applied Environmental Microbiology, 67: 5431-5436. Go to original source... Go to PubMed...

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