Czech J. Anim. Sci., 2007, 52(11):407-413 | DOI: 10.17221/2321-CJAS

Supplemental wheat bran and microbial phytase could replace inorganic phosphorus in laying hen diets

J.H. Yao1, J.C. Han1, S.Y. WU1, M. XU1, L.L. Zhong1, Y.R. Liu2, Y.J. Wang2
1 College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi, China
2 Shenzhen Kondarl (Gaoling) Feed Co., Ltd., Gaoling, Shaanxi, China

An experiment was conducted to determine effects of wheat bran (WB) phytase on production performance and nutrient utilization in laying hens. Three hundred and seventy-five Lohmann hens at 32 weeks of age were randomly allotted to treatments of fifteen hens per pen with five pens per treatment. Five experimental diets were formulated. Diet one (control) contained 0.19% inorganic phosphate (Pi) from dicalcium phosphate. On the basis of diet 1, diet 2 and 3 were formulated to contain WB 5% and 10%, respectively. In diet 4 and 5, the WB was fixed at the level of 10% with Pi level adjusted to 0.14% in diet 4 and completely replaced with 500 U/kg microbial phytase in diet 5. The results showed that treatment three improved egg yield (P = 0.142), feed conversion ratio (FCR) (P = 0.011), utilization of crude protein (CP) (P = 0.060) and total phosphorus (tP) (P < 0.001), and serum Pi concentration (P = 0.016) compared with the control. Ten percent of WB replacing 0.05% Pi did not influence either egg yield or nutrient utilization. Compared with the control, treatment five improved FCR (P = 0.011) and utilization of CP (P = 0.060) and tP (P < 0.001), but did not influence either performance or serum parameters. The current study suggests that wheat bran could be used successfully in laying hen diets and wheat bran and microbial phytase supplemented together could replace inorganic phosphate completely.

Keywords: wheat bran phytase; laying hens; production performance; nutrient utilization

Published: November 30, 2007  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Yao JH, Han JC, WU SY, XU M, Zhong LL, Liu YR, Wang YJ. Supplemental wheat bran and microbial phytase could replace inorganic phosphorus in laying hen diets. Czech J. Anim. Sci.. 2007;52(11):407-413. doi: 10.17221/2321-CJAS.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Augspurger N.R., Webel D.M., Lei X.G., Baber D.H. (2003): Efficacy of an E. coli phytase expressed in yeast for releasing phytate-bound phosphorus in young chicks and pigs. J. Anim. Sci., 81, 474-483. Go to original source... Go to PubMed...
    2. Cavalcanti W.B., Behnke K.C. (2004): Effect of wheat bran phytase subjected to different conditioning temperatures on phosphorus utilization by broiler chicks based on body weight and toe ash measurements. Int. J. Poult. Sci., 3, 215-219. Go to original source...
    3. Duncan D.B. (1955): Multiple range and multiple F-tests. Biometries, 11, 1-42. Go to original source...
    4. Eeckhout W., Depaepe M. (1994): Total phosphorus, phytate-phosphorus and phytase activity in plant feedstuffs. Anim. Feed Sci. Technol., 47, 19-29. Go to original source...
    5. Han Y.M., Yang F., Zhou A.G., Miller E.R., Ku P.K., Hogberg M.G., Lei X.G. (1997): Supplemental phytases of microbial and cereal sources improve dietary phytate phosphorus utilization by pigs from weaning through finishing. J. Anim. Sci., 75, 1017-1025. Go to original source... Go to PubMed...
    6. Han Y.M., Roneker K.R., Pond W.G., Lei X.G. (1998): Adding wheat middlings, microbial phytase, and citric acid to corn-soybean meal diets for growing pigs may replace inorganic phosphorus supplementation. J. Anim. Sci., 76, 2649-2656. Go to original source... Go to PubMed...
    7. Ketaren P.P., Batterham E.S., Dettmann E.B., Farrell D.J. (1993): Phosphorus studies in pigs. 3. Effect of phytase supplementation on the digestibility and availability of phosphorus in soyabean meal for grower pigs. Brit. J. Nutr., 70, 289-311. Go to original source... Go to PubMed...
    8. Langlois A., Corring T., Fevrier C. (1987): Effects of wheat bran on exocrine pancreas secretion in the pig. Reprod. Nutr. Dev., 27, 929-939. Go to original source... Go to PubMed...
    9. McCance R.A., Widdowson E.M. (1944): Activity of the phytase in different cereals and its resistance to dry heat. Nature, 153, 650-650. Go to original source...
    10. Mroz Z., Jongbloed A.W., Kemme P.A. (1994): Apparent digestibility and retention of nutrients bound to phytate complexes as influenced by microbial phytase and feeding regimen in pigs. J. Anim. Sci., 72, 126-132. Go to original source... Go to PubMed...
    11. Paik I.K. (2003): Application of phytase, microbial or plant origin, to reduce phosphorus excretion in poultry production. Asian-Australas. J. Anim. Sci., 1, 124-135. Go to original source...
    12. Pallauf J., Rimbach G., Pippig S., Schindler B., Most E. (1994): Effect of phytase supplementation to a phytaterich diet based on wheat, barley and soya on the bioavailability of dietary phosphorus, calcium, magnesium, zinc and protein in piglets. Agribiol. Res., 47, 39-48.
    13. Pointillart A. (1991): Enhancement of phosphorus utilization in growing pigs fed phytate-rich diets by using rye bran. J. Anim. Sci., 69, 1109-1115. Go to original source... Go to PubMed...
    14. Pointillart A., Fontaine N., Thomasset M. (1984): Phytate phosphorus utilization and intestinal phosphatases in pigs fed low phosphorus: wheat or corn diets. Nutr. Rep. Int., 29, 473-483.
    15. Pointillart A., Fourdin A., Fontaine N. (1987): Importance of cereal phytase activity for phytate phosphorus utilization by growing pigs fed diets containing triticale or corn. J. Nutr., 117, 907-913. Go to original source... Go to PubMed...
    16. Punna S., Roland D.A. (1999): Variation in phytate phosphorus utilization within the same broiler strain. J. Appl. Poult. Res., 8, 10-15. Go to original source...
    17. Ravindran V., Selle P.H., Ravindran G., Morel P.C.H., Kies A.K., Bryden W.L. (2001): Microbial phytase improves performance, apparent metabolizable energy and ileal amino acid digestibility of broiler fed a lysine-deficient diet. Poult. Sci., 80, 338-344. Go to original source... Go to PubMed...
    18. Roberson K.D., Kalbfleisch J.L., Pan W., Applegate T.J., Rosenstein D.S. (2005): Comparison of wheat bran phytase and a commercially available phytase on turkey tom performance and litter phosphorus content. Int. J. Poult. Sci., 5, 244-249. Go to original source...
    19. Roche Vitamins (1999): Determination of phytase activity in tel quell, formulated and feed samples. Method PHY-101/03E, Roche Vitamins Ltd., Basel, Switzerland.
    20. Rutherfurd S.M., Chung T.K., Morel P.C., Moughan P.J. (2004): Effect of microbial phytase on ileal digestibility of phytate phosphorus, total phosphorus, and amino acids in a low-phosphorus diet for broilers. Poult. Sci., 83, 61-68. Go to original source... Go to PubMed...
    21. SAS (2001): SAS User's Guide: Statistics. Version. 8.02 ed. SAS Inst., Inc., Cary, NC.
    22. Simgh B., Sedeh H.G. (1979): Characteristics of phytase and its relationship to acid phosphatase and certain minerals in triticale. Cereal Chem., 56, 267-272.
    23. Steiner T., Mosenthin R., Zimmermannb B., Greiner R., Roth S. (2007): Distribution of phytase activity, total phosphorus and phytate phosphorus in legume seeds, cereals and cereal by-products as influenced by harvest year and cultivar. Anim. Feed Sci. Technol., 133, 320-334. Go to original source...
    24. Valette P., Corring T., Juste C., Levenez F. (1989): Shortterm effects of wheat bran incorporation into the diets on bile secretion in the pig. Nutr. Rep. Int., 40, 1059-1071.
    25. Viveros A., Centeno C., Brenes A., Canales R., Lozano A. (2000): Phytase and acid phosphatase activities in plant feedstuffs. J. Agric. Food Chem., 48, 4009-4013. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content