Vet Med - Czech, 2008, 53(6):283-306 | DOI: 10.17221/1859-VETMED

Detection methods for Mycobacterium avium subsp paratuberculosis in milk and milk products: a review

I. Slana1, F. Paolicchi2, B. Janstova3, P. Navratilova3, I. Pavlik1
1 Veterinary Research Institute, Brno, Czech Republic
2 National Institute of Agricultural Technology, Balcarce, Argentina
3 University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic

Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis, a disease with considerable economic impact, principally on dairy cattle herds. Animals with paratuberculosis shed viable MAP especially in their milk, faeces and semen. MAP may have a role in the development of Crohn's disease in humans via the consumption of contaminated milk and milk products. The current methods of milk pasteurization are not sufficient to kill all MAP cells present in milk and MAP has been cultured from raw or pasteurized milk and isolated from cheese. The purpose of the present study was to review the different methods used for detection of MAP in milk and milk products. We analyze the current methods for direct or non direct identification of MAP and culture and molecular biology methods that can be applied to milk and milk products.

Keywords: PCR; cultivation; IS900; ELISA; MELISA; food safety; potential zoonosis; Johne's disease

Published: June 30, 2008  Show citation

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Slana I, Paolicchi F, Janstova B, Navratilova P, Pavlik I. Detection methods for Mycobacterium avium subsp paratuberculosis in milk and milk products: a review. Vet Med-Czech. 2008;53(6):283-306. doi: 10.17221/1859-VETMED.
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    References

    1. Alexejeff-Goloff N.A. (1935): Journal of Comparative Pathology, 48, 81. Go to original source...
    2. Altic L.C., Rowe M.T., Grant I.R. (2007): UV Light inactivation of Mycobacterium avium subsp. paratuberculosis in milk as assessed by FASTPlaqueTB phage assay and culture. Applied and Environmental Microbiology, 73, 3728-3733. Go to original source... Go to PubMed...
    3. Anonymous (2004a): Council Regulation (EC) No. 852/2004 of the European Parliament and of the Council of 29 April 2004 on the hygiene of foodstuffs. OJ L 139, 30.4.2004, p. 1-54.
    4. Anonymous (2004b): Council Regulation (EC) No. 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin. OJ L 139, 30.4.2004, p. 55-205.
    5. Anonymous (2006): Council Regulation (EC) No. 1662/2006 of 6 November 2006 amending Regulation (EC) No 853/2004 of the European Parliament and of the Council laying down specific hygiene rules for food of animal origin. OJ L 320, 18.11.2006, p. 1-10.
    6. Ayele W.Y., Bartos M., Svastova P., Pavlik I. (2004): Distribution of Mycobacterium avium subsp. paratuberculosis in organs of naturally infected bull-calves and breeding bulls. Veterinary Microbiology, 103, 209-217. Go to original source... Go to PubMed...
    7. Ayele W.Y., Svastova P., Roubal P., Bartos M., Pavlik I. (2005): Mycobacterium avium subspecies paratuberculosis cultured from locally and commercially pasteurized cow's milk in the Czech Republic. Applied and Environmental Microbiology, 71, 1210-1214. Go to original source... Go to PubMed...
    8. Bendixen P.H., Bloch B., Jorgensen J.B. (1981): Lack of intracellular degradation of Mycobacterium paratuberculosis by bovine macrophages infected in vitro and in vivo: light microscopic and electron microscopic observations. American Journal of Veterinary Research, 42, 109-113.
    9. Bosshard C., Stephan R., Tasara T. (2006): Application of an F57 sequence-based real-time PCR assay for Mycobacterium paratuberculosis detection in bulk tank raw milk and slaughtered healthy dairy cows. Journal of Food Protection, 69, 1662-1667. Go to original source... Go to PubMed...
    10. Chiodini R.J., Vankruiningen H.J. (1986): The prevalence of paratuberculosis in culled New-England cattle. Cornell Veterinarian, 76, 91-104.
    11. Chiodini R.J., Vankruiningen H.J., Merkal R.S. (1984): Ruminant paratuberculosis (Johnes disease) - the current status and future-prospects. Cornell Veterinarian, 74, 218-262.
    12. Cho M., Yoon J. (2007): The application of bioluminescence assay with culturing for evaluating quantitative disinfection performance. Water Research, 41, 741-746. Go to original source... Go to PubMed...
    13. Clark D.L. Jr., Anderson J.L., Koziczkowski J.J., Ellingson J.L. (2006): Detection of Mycobacterium avium subspecies paratuberculosis genetic components in retail cheese curds purchased in Wisconsin and Minnesota by PCR. Molecular and Cellular Probes, 20, 197-202. Go to original source... Go to PubMed...
    14. Collins M.T., Wells S.J., Petrini K.R., Collins J.E., Schultz R.D., Whitlock R.H. (2005): Evaluation of five antibody detection tests for diagnosis of bovine paratuberculosis. Clinical and Diagnostic Laboratory Immunology, 12, 685-692. Go to original source... Go to PubMed...
    15. Corti S., Stephan R. (2002): Detection of Mycobacterium avium subspecies paratuberculosis specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland. BMC. Microbiology, 2, 15. Go to original source... Go to PubMed...
    16. D'Haese E., Dumon I., Werbrouck H., De J.V., Herman L. (2005): Improved detection of Mycobacterium paratuberculosis in milk. The Journal of Dairy Research, 72, Spec No., 125-128. Go to original source... Go to PubMed...
    17. Djonne B., Jensen M.R., Grant I.R., Holstad G. (2003): Detection by immunomagnetic PCR of Mycobacterium avium subsp. paratuberculosis in milk from dairy goats in Norway. Veterinary Microbiology, 92, 135-143. Go to original source... Go to PubMed...
    18. Donaghy J.A., Linton M., Patterson M.F., Rowe M.T. (2007): Effect of high pressure and pasteurization on Mycobacterium avium ssp. paratuberculosis in milk. Letters in Applied Microbiology, 45, 154-159. Go to original source... Go to PubMed...
    19. Dundee L., Grant I.R., Ball H.J., Rowe M.T. (2001): Comparative evaluation of four decontamination protocols for the isolation of Mycobacterium avium subsp. paratuberculosis from milk. Letters in Applied Microbiology, 33, 173-177. Go to original source... Go to PubMed...
    20. Ellingson J.L.E., Bolin C.A., Stabel J.R. (1998): Identification of a gene unique to Mycobacterium avium subspecies paratuberculosis and application to diagnosis of paratuberculosis. Molecular and Cellular Probes, 12, 133-142. Go to original source... Go to PubMed...
    21. Ellingson J.L., Anderson J.L., Koziczkowski J.J., Radcliff R.P., Sloan S.J., Allen S.E., Sullivan N.M. (2005): Detection of viable Mycobacterium avium subsp. paratuberculosis in retail pasteurized whole milk by two culture methods and PCR. Journal of Food Protection, 68, 966-972. Go to original source... Go to PubMed...
    22. Englund S., Bolske G., Johansson K.E. (2002): An IS900like sequence found in a Mycobacterium sp. other than Mycobacterium avium subsp. paratuberculosis. FEMS Microbiology Letters, 209, 267-271. Go to original source... Go to PubMed...
    23. Gao A., Mutharia L., Chen S., Rahn K., Odumeru J. (2002): Effect of pasteurization on survival of Mycobacterium paratuberculosis in milk. Journal of Dairy Science, 85, 3198-3205. Go to original source... Go to PubMed...
    24. Gao A., Odumeru J., Raymond M., Mutharia L. (2005): Development of improved method for isolation of Mycobacterium avium subsp. paratuberculosis from bulk tank milk: effect of age of milk, centrifugation, and decontamination. Canadian Journal of Veterinary Research, 69, 81-87.
    25. Gao A., Mutharia L., Raymond M., Odumeru J. (2007): Improved template DNA preparation procedure for detection of Mycobacterium avium subsp. paratuberculosis in milk by PCR. Journal of Microbiological Methods, 69, 417-420. Go to original source... Go to PubMed...
    26. Geue L., Kohler H., Klawonn W., Drager K., Hess R.G., Conraths F.J. (2007): The suitability of ELISA for the detection of antibodies against Mycobacterium avium ssp. paratuberculosis in bulk milk samples from Rhineland-Palatinate (in German). Berliner and Munchenen Tierarztliche Wochenschrift, 120, 67-78.
    27. Giese S.B., Ahrens P. (2000): Detection of Mycobacterium avium subsp. paratuberculosis in milk from clinically affected cows by PCR and culture. Veterinary Microbiology, 77, 291-297. Go to original source... Go to PubMed...
    28. Gilmour N.J.L. (1976): Pathogenesis, diagnosis and control of Johnes disease. Veterinary Record, 99, 433-434. Go to original source... Go to PubMed...
    29. Gilmour, N.J.L. (1985): Mycobacterium paratuberculosis. In: Meissner G. (ed.): Handbuch der bakteriellen Infektionen bei Tieren (in German). Band V. VEB Gustav Fischer Verlag, Jena. 281-313.
    30. Grant I.R. (2006): Mycobacterium avium ssp paratuberculosis in foods: current evidence and potential consequences. International Journal of Dairy Technology, 59, 112-117. Go to original source...
    31. Grant I.R., Rowe M.T. (2004): Effect of chemical decontamination and refrigerated storage on the isolation of Mycobacterium avium subsp. paratuberculosis from heat-treated milk. Letters in Applied Microbiology, 38, 283-288. Go to original source... Go to PubMed...
    32. Grant I.R., Ball H.J., Rowe M.T. (1998): Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation. Applied and Environmental Microbiology, 64, 3153-3158. Go to original source... Go to PubMed...
    33. Grant I.R., Pope C.M., O'Riordan L.M., Ball H.J., Rowe M.T. (2000): Improved detection of Mycobacterium avium subsp. paratuberculosis in milk by immunomagnetic PCR. Veterinary Microbiology, 77, 369-378. Go to original source... Go to PubMed...
    34. Grant I.R., Rowe M.T., Dundee L., Hitchings E. (2001): Mycobacterium avium ssp. paratuberculosis: its incidence, heat resistance and detection in milk and dairy products. International Journal of Dairy Technology, 54, 2-13. Go to original source...
    35. Grant I.R., Ball H.J., Rowe M.T. (2002a): Incidence of Mycobacterium paratuberculosis in bulk raw and commercially pasteurized cows' milk from approved dairy processing establishments in the United Kingdom. Applied and Environmental Microbiology, 68, 2428-2435. Go to original source... Go to PubMed...
    36. Grant I.R., Hitchings E.I., McCartney A., Ferguson F., Rowe M.T. (2002b): Effect of commercial-scale hightemperature, short-time pasteurization on the viability of Mycobacterium paratuberculosis in naturally infected cows' milk. Applied and Environmental Microbiology, 68, 602-607. Go to original source... Go to PubMed...
    37. Grant I.R., Kirk R.B., Hitchings E., Rowe M.T. (2003): Comparative evaluation of the MGIT and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk. Journal of Applied Microbiology, 95, 196-201. Go to original source... Go to PubMed...
    38. Grant I.R., Williams A.G., Rowe M.T., Muir D.D. (2005): Efficacy of various pasteurization time-temperature conditions in combination with homogenization on inactivation of Mycobacterium avium subsp. paratuberculosis in milk. Applied and Environmental Microbiology, 71, 2853-2861. Go to original source... Go to PubMed...
    39. Green E.P., Tizard M.L., Moss M.T., Thompson J., Winterbourne D.J., McFadden J.J., Hermon-Taylor J. (1989): Sequence and characteristics of IS900, an insertion element identified in a human Crohn's disease isolate of Mycobacterium paratuberculosis. Nucleic Acids Research, 17, 9063-9073. Go to original source... Go to PubMed...
    40. Gumber S., Eamens G., Whittington R.J. (2006): Evaluation of a Pourquier ELISA kit in relation to agar gel immunodiffusion (AGID) test for assessment of the humoral immune response in sheep and goats with and without Mycobacterium paratuberculosis infection. Veterinary Microbiology, 115, 91-101. Go to original source... Go to PubMed...
    41. Gupta U.D., Katoch K., Natarajan M., Sharma V.D., Sharma R.K., Shivannavar C.T., Katoch V.M. (1997): Viability determination of M.leprae: comparison of normal mouse foot pad and bacillary ATP bioluminescence assay. Acta Leprologica, 10, 209-212. Go to PubMed...
    42. Hammer P., Wiesner C., Walte H.-G., Knappstein K., Teufel P. (2002): Heat resistance of Mycobacterium avium subsp. paratuberculosis in raw milk tested in pilot plant pasteuriser. Kieler Milchwirtschaftlich, 54, 275-303.
    43. Hardin L.E., Thorne J.G. (1996): Comparison of milk with serum ELISA for the detection of paratuberculosis in dairy cows. Journal of the American Veterinary Medical Association, 209, 120-122. Go to original source... Go to PubMed...
    44. Harris N.B., Barletta R.G. (2001): Mycobacterium avium subsp. paratuberculosis in Veterinary Medicine. Clinical Microbiology Reviews, 14, 489-512. Go to original source... Go to PubMed...
    45. Hasonova L., Pavlik I. (2006): Economic impact of paratuberculosis in dairy cattle herds: a review. Veterinarni Medicina, 51 (5), 193-211. http://www.vri.cz/docs/vetmed/51-5-193.pdf Go to original source...
    46. Hendrick S., Duffield T., Leslie K., Lissemore K., Archambault M., Kelton D. (2005a): The prevalence of milk and serum antibodies to Mycobacterium avium subspecies paratuberculosis in dairy herds in Ontario. Canadian Veterinary Journal-Revue Veterinaire Canadienne, 46, 1126-1129.
    47. Hendrick S.H., Duffield T.F., Kelton D.F., Leslie K.E., Lissemore K.D., Archambault M. (2005b): Evaluation of enzyme-linked immunosorbent assays performed on milk and serum samples for detection of paratuberculosis in lactating dairy cows. Journal of the American Veterinary Medical Association, 226, 424-428. Go to original source... Go to PubMed...
    48. Hendrick S.H., Duffield T.F., Leslie K.E., Lissemore K. D., Archambault M., Bagg R., Dick P., Kelton D.F. (2006): Monensin might protect Ontario, Canada dairy cows from paratuberculosis milk-ELISA positivity. Preventive Veterinary Medicine, 76, 237-248. Go to original source... Go to PubMed...
    49. Hermon-Taylor J., Bull T. (2002): Crohn's disease caused by Mycobacterium avium subspecies paratuberculosis: a public health tragedy whose resolution is long overdue. Journal of Medical Microbiology, 51, 3-6. Go to original source... Go to PubMed...
    50. Hole N.H. (1958): Johne's disease. Advances in Veterinary Science, 4, 341-387.
    51. Holsinger V.H., Rajkowski K.T., Stabel J.R. (1997): Milk pasteurization and safety: a brief history and update. Revue Scientifique et Technique, 16, 441-451. Go to original source... Go to PubMed...
    52. Hruska K., Bartos M., Kralik P., Pavlik I. (2005): Mycobacterium avium subsp paratuberculosis in powdered infant milk: paratuberculosis in cattle - the public health problem to be solved. Veterinarni Medicina, 50, 327-335. http://www.vri.cz/docs/vetmed/50-8327.pdf Go to original source...
    53. Ikonomopoulos J., Pavlik I., Bartos M., Svastova P., Ayele W.Y., Roubal P., Lukas J., Cook N., Gazouli M. (2005): Detection of Mycobacterium avium subsp. paratuberculosis in retail cheeses from Greece and the Czech Republic. Applied and Environmental Microbiology, 71, 8934-8936. Go to original source... Go to PubMed...
    54. Jawhara S., Mordon S. (2004): In vivo imaging of bioluminescent Escherichia coli in a cutaneous wound infection model for evaluation of an antibiotic therapy. Antimicrobial Agents and Chemotherapy, 48, 3436-3441. Go to original source... Go to PubMed...
    55. Jayarao B.M., Pillai S.R., Wolfgang D.R., Griswold D.R., Rossiter C.A., Tewari D., Burns C.M., Hutchinson L.J. (2004): Evaluation of IS900-PCR assay for detection of Mycobacterium avium subspecies paratuberculosis infection in cattle using quarter milk and bulk tank milk samples. Foodborne Pathogens and Disease, 1, 17-26. Go to original source... Go to PubMed...
    56. Jorgensen J.B., Jensen P.T. (1978): Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Mycobacterium paratuberculosis in cattle. Acta Veterinaria Scandinavica, 19, 310-312. Go to original source... Go to PubMed...
    57. Keswani J., Frank J.F. (1998): Thermal inactivation of Mycobacterium paratuberculosis in milk. Journal of Food Protection, 61, 974-978. Go to original source... Go to PubMed...
    58. Khare S., Ficht T.A., Santos R.L., Romano J., Ficht A.R., Zhang S., Grant I.R., Libal M., Hunter D., Adams L.G. (2004): Rapid and sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separation-conventional PCR and real-time PCR. Journal of Clinical Microbiology, 42, 1075-1081. Go to original source... Go to PubMed...
    59. Klausen J., Huda A., Ekeroth L., Ahrens P. (2003): Evaluation of serum and milk ELISAs for paratuberculosis in Danish dairy cattle. Preventive Veterinary Medicine, 58, 171-178. Go to original source... Go to PubMed...
    60. Koenig G.J., Hoffsis G.F., Shulaw W.P., Bech-Nielsen S., Rings D.M., St Jean G. (1993): Isolation of Mycobacterium paratuberculosis from mononuclear cells in tissues, blood, and mammary glands of cows with advanced paratuberculosis. American Journal of Veterinary Research, 54, 1441-1445. Go to original source...
    61. Larsen A.B., Vardaman T.H., Merkal R.S. (1963): An extended study of a herd of cattle naturally infected with Johnes disease. 2. Significance of complement-fixation test. American Journal of Veterinary Research, 24, 948.
    62. Li L.L., Bannantine J.P., Zhang Q., Amonsin A., May B. J., Alt D., Banerji N., Kanjilal S., Kapur V. (2005): The complete genome sequence of Mycobacterium avium subspecies paratuberculosis. Proceedings of the National Academy of Sciences of the United States of America, 102, 12344-12349. Go to original source... Go to PubMed...
    63. Lombard J.E., Byrem T.M., Wagner B.A., McCluskey B.J. (2006a): Comparison of milk and serum enzyme-linked immunosorbent assays for diagnosis of Mycobacterium avium subspecies paratuberculosis infection in dairy cattle. Journal of Veterinary Diagnostic Investigation, 18, 448-458. Go to original source... Go to PubMed...
    64. Lombard J.E., Wagner B.A., Smith R.L., McCluskey B.J., Harris B.N., Payeur J.B., Garry F.B., Salman M.D. (2006b): Evaluation of environmental sampling and culture to determine Mycobacterium avium subspecies paratuberculosis distribution and herd infection status on US dairy operations. Journal of Dairy Science, 89, 4163-4171. Go to original source... Go to PubMed...
    65. Martin-Casabona N., Xairo M.D., Gonzalez T., Rossello J., Arcalis L. (1997): Rapid method for testing susceptibility of Mycobacterium tuberculosis by using DNA probes. Journal of Clinical Microbiology, 35, 2521- 2525. Go to original source... Go to PubMed...
    66. McDonald W.L., O'Riley K.J., Schroen C.J., Condron R.J. (2005): Heat inactivation of Mycobacterium avium subsp. paratuberculosis in milk. Applied and Environmental Microbiology, 71, 1785-1789. Go to original source... Go to PubMed...
    67. Metzger-Boddien C., Khaschabi D., Schonbauer M., Boddien S., Schlederer T., Kehle J. (2006): Automated high-throughput immunomagnetic separation-PCR for detection of Mycobacterium avium subsp. paratuberculosis in bovine milk. International Journal of Food Microbiology, 110, 201-208. Go to original source... Go to PubMed...
    68. Millar D., Ford J., Sanderson J., Withey S., Tizard M., Doran T., Hermon-Taylor J. (1996): IS900 PCR to detect Mycobacterium paratuberculosis in retail supplies of whole pasteurized cows' milk in England and Wales. Applied and Environmental Microbiology, 62, 3446- 3452. Go to original source... Go to PubMed...
    69. Muehlherr J.E., Zweifel C., Corti S., Blanco J.E., Stephan R. (2003): Microbiological quality of raw goat's and ewe's bulk-tank milk in Switzerland. Journal of Dairy Science, 86, 3849-3856. Go to original source... Go to PubMed...
    70. Muhammed S.I., Eliasson E.C. (1979): Prevalence of antibodies to Mycobacterium johnei in colostrum deprived lambs. Veterinary Record, 105, 11-12. Go to original source... Go to PubMed...
    71. Naser S.A., Schwartz D., Shafran I. (2000): Isolation of Mycobacterium avium subsp paratuberculosis from breast milk of Crohn's disease patients. The American Journal of Gastroenterology, 95, 1094-1095. Go to original source... Go to PubMed...
    72. Nielsen S.S., Ersboll A.K. (2006): Age at occurrence of Mycobacterium avium subspecies paratuberculosis in naturally infected dairy cows. Journal of Dairy Science, 89, 4557-4566. Go to original source... Go to PubMed...
    73. Nielsen S.S., Thamsborg S.M., Houe H., Bitsch V. (2000): Bulk-tank milk ELISA antibodies for estimating the prevalence of paratuberculosis in Danish dairy herds. Preventive Veterinary Medicine, 44, 1-7. Go to original source... Go to PubMed...
    74. Nielsen S.S., Grohn Y.T., Enevoldsen C. (2002): Variation of the milk antibody response to paratuberculosis in naturally infected dairy cows. Journal of Dairy Science, 85, 2795-2802. Go to original source... Go to PubMed...
    75. O'Doherty A., O'Grady D., Smith T., Egan J. (2002): Mycobacterium avium subsp paratuberculosis in pasteurized and unpasteurized milk in the Republic of Ireland. Irish Journal of Agricultural and Food Research, 41, 117-121.
    76. Odumeru J., Gao A., Chen S., Raymond M., Mutharia L. (2001): Use of the bead beater for preparation of Mycobacterium paratuberculosis template DNA in milk. Canadian Journal of Veterinary Research, 65, 201- 205.
    77. O'Mahony J., Hill C. (2002): A real time PCR assay for the detection and quantitation of Mycobacterium avium subsp. paratuberculosis using SYBR Green and the Light Cycler. Journal of Microbiological Methods, 51, 283-293. Go to original source... Go to PubMed...
    78. O'Mahony J., Hill C. (2004): Rapid real-time PCR assay for detection and quantitation of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milk. Applied and Environmental Microbiology, 70, 4561-4568. Go to original source... Go to PubMed...
    79. O'Reilly C.E., O'Connor L., Anderson W., Harvey P., Grant I.R., Donaghy J., Rowe M., O'Mahony P. (2004): Surveillance of bulk raw and commercially pasteurized cows' milk from approved Irish liquid-milk pasteurization plants to determine the incidence of Mycobacterium paratuberculosis. Applied and Environmental Microbiology, 70, 5138-5144. Go to original source... Go to PubMed...
    80. Paolicchi F.A., Zumarraga M.J., Gioffre A., Zamorano P., Morsella C., Verna A., Cataldi A., Alito A., Romano M. (2003): Application of different methods for the diagnosis of paratuberculosis in a dairy cattle herd in Argentina. Journal of Veterinary Medicine Series BInfectious Diseases and Veterinary Public Health, 50, 20-26. Go to original source... Go to PubMed...
    81. Pavlik I., Rozsypalova Z., Vesely T., Bartl J., Matlova L., Vrbas V., Valent L., Rajsky D., Mracko I., Hirko M., Miskovic P. (2000): Control of paratuberculosis in five cattle farms by serological tests and faecal culture during the period 1990-1999. Veterinarni Medicina, 45, 61-70.
    82. Pearce L.E., Truong H.T., Crawford R.A., Yates G.F., Cavaignac S., de Lisle G.W. (2001): Effect of turbulentflow pasteurization on survival of Mycobacterium avium subsp. paratuberculosis added to raw milk. Applied and Environmental Microbiology, 67, 3964- 3969. Go to original source... Go to PubMed...
    83. Pillai S.R., Jayarao B.M. (2002): Application of IS900 PCR for detection of Mycobacterium avium subsp. paratuberculosis directly from raw milk. Journal of Dairy Science, 85, 1052-1057. Go to original source... Go to PubMed...
    84. Pinedo P.J., Rae D.O., Williams J.E., Donovan G.A., Melendez P., Buergelt C.D. (2008): Association among results of serum ELISA, faecal culture and nested PCR on milk, blood and faeces for the detection of paratuberculosis in dairy cows. Transboundary Emerging Diseases, 55, 125-133. Go to original source... Go to PubMed...
    85. Poupart P., Coene M., Van Heuverswyn H., Cocito C. (1993): Preparation of a specific RNA probe for detection of Mycobacterium paratuberculosis and diagnosis of Johne's disease. Journal of Clinical Microbiology, 31, 1601-1605. Go to original source... Go to PubMed...
    86. Rademaker J.L., Vissers M.M., Te Giffel M.C. (2007): Effective heat inactivation of Mycobacterium avium subsp. paratuberculosis in raw milk contaminated with naturally infected feces. Applied and Environmental Microbiology, 73, 4185-4190. Go to original source... Go to PubMed...
    87. Richards W.D. (1990): A new, inexpensive test for detecting paratuberculosis in dairy herds. The paratuberculosis Newsletters, 21, 7-8.
    88. Rodriguez-Lazaro D., D'Agostino M., Pla M., Cook N. (2004): Construction strategy for an internal amplification control for real-time diagnostic assays using nucleic Acid sequence-based amplification: development and clinical application. Journal of Clinical Microbiology, 42, 5832-5836. Go to original source... Go to PubMed...
    89. Rodriguez-Lazaro D., D'Agostino M., Herrewegh A., Pla M., Cook N., Ikonomopoulos J. (2005): Real-time PCRbased methods for detection of Mycobacterium avium subsp. paratuberculosis in water and milk. International Journal of Food Microbiology, 101, 93-104. Go to original source... Go to PubMed...
    90. Rosseels V., Roupie V., Zinniel D., Barletta R.G., Huygen K. (2006): Development of luminescent Mycobacterium avium subsp paratuberculosis for rapid screening of vaccine candidates in mice. Infection and Immunity, 74, 3684-3686. Go to original source... Go to PubMed...
    91. Ruzante J.M., Smith,W.L., Gardner I.A., Thornton C.G., Cullor J.S. (2006): Modified culture protocol for isolation of Mycobacterium avium subsp. paratuberculosis from raw milk. Foodborne Pathogens Disease, 3, 457- 460. Go to original source... Go to PubMed...
    92. Salgado M., Kruze J., Collins M.T. (2007): Diagnosis of paratuberculosis by fecal culture and ELISA on milk and serum samples in two types of Chilean dairy goat herds. Journal of Veterinary Diagnostic Investigation, 19, 99-102. Go to original source... Go to PubMed...
    93. Sasahara K.C., Gray M.J., Shin S.J., Boor K.J. (2004): Detection of viable Mycobacterium avium subsp. paratuberculosis using luciferase reporter systems. Foodborne Pathogens and Disease, 1, 258-266. Go to original source... Go to PubMed...
    94. Singh S.V., Vihan V.S. (2004): Detection of Mycobacterium avium subspecies paratuberculosis in goat milk. Small Ruminant Research, 54, 231-235. Go to original source...
    95. Singh S.V., Singh A.V., Singh R., Sandhu K.S., Singh P. K., Sohal J.S., Gupta V.K., Vihan V.S. (2007): Evaluation of highly sensitive indigenous milk ELISA kit with fecal culture, milk culture and fecal-PCR for the diagnosis of bovine Johne's disease (BJD) in India. Comparative Immunology Microbiology and Infectious Diseases, 30, 175-186. Go to original source... Go to PubMed...
    96. Smith H.W. (1960): The Examination of milk for the presence of Mycobacterium johnei. Journal of Pathology and Bacteriology, 80, 440-442. Go to original source...
    97. Spahr U., Schafroth K. (2001): Fate of Mycobacterium avium subsp. paratuberculosis in Swiss hard and semihard cheese manufactured from raw milk. Applied and Environmental Microbiology, 67, 4199-4205. Go to original source... Go to PubMed...
    98. Stabel J.R. (2001): On-farm batch pasteurization destroys Mycobacterium paratuberculosis in waste milk. Journal of Dairy Science, 84, 524-527. Go to original source... Go to PubMed...
    99. Stabel J.R., Lambertz A. (2004): Efficacy of pasteurization conditions for the inactivation of Mycobacterium avium subsp. paratuberculosis in milk. Journal of Food Protection, 67, 2719-2726. Go to original source... Go to PubMed...
    100. Stabel J.R., Steadham E.M., Bolin C.A. (1997): Heat inactivation of Mycobacterium paratuberculosis in raw milk: are current pasteurization conditions effective? Applied and Environmental Microbiology, 63, 4975-4977. Go to original source... Go to PubMed...
    101. Stabel J.R., Wells S.J., Wagner B.A. (2002): Relationships between fecal culture, ELISA, and bulk tank milk test results for Johne's disease in US dairy herds. Journal of Dairy Science, 85, 525-531. Go to original source... Go to PubMed...
    102. Stabel J.R., Hurd S., Calvente L., Rosenbusch R.F. (2004): Destruction of Mycobacterium paratuberculosis, Salmonella spp., and Mycoplasma spp. in raw milk by a commercial on-farm high-temperature, short-time pasteurizer. Journal of Dairy Science, 87, 2177-2183. Go to original source... Go to PubMed...
    103. Stanley E.C., Mole R.J., Smith R.J., Glenn S.M., Barer M.R., McGowan M., Rees C.E.D. (2007): Development of a new, combined rapid method using phage and PCR for detection and identification of viable Mycobacterium paratuberculosis bacteria within 48 hours. Applied and Environmental Microbiology, 73, 1851-1857. Go to original source... Go to PubMed...
    104. Stephan R., Schumacher S., Tasara T., Grant I.R. (2007): Prevalence of Mycobacterium avium subspecies paratuberculosis in Swiss raw milk cheeses collected at the retail level. Journal of Dairy Science, 90, 3590-3595. Go to original source... Go to PubMed...
    105. Stratmann J., Strommenger B., Stevenson K., Gerlach G.F. (2002): Development of a peptide-mediated capture PCR for detection of Mycobacterium avium subsp. paratuberculosis in milk. Journal of Clinical Microbiology, 40, 4244-4250. Go to original source... Go to PubMed...
    106. Stratmann J., Dohmann K., Heinzmann J., Gerlach G.F. (2006): Peptide aMptD-mediated capture PCR for detection of Mycobacterium avium subsp. paratuberculosis in bulk milk samples. Applied and Environmental Microbiology, 72, 5150-5158. Go to original source... Go to PubMed...
    107. Streeter R.N., Hoffsis G.F., Bech-Nielsen S., Shulaw W. P., Rings D.M. (1995): Isolation of Mycobacterium paratuberculosis from colostrum and milk of subclinically infected cows. American Journal of Veterinary Research, 56, 1322-1324. Go to original source...
    108. Strommenger B., Stevenson K., Gerlach G.F. (2001): Isolation and diagnostic potential of ISMav2, a novel insertion sequence-like element from Mycobacterium avium subspecies paratuberculosis. FEMS Microbiology Letters, 196, 31-37. Go to original source... Go to PubMed...
    109. Sung N., Collins M.T. (1998): Thermal tolerance of Mycobacterium paratuberculosis. Applied and Environmental Microbiology, 64, 999-1005. Go to original source... Go to PubMed...
    110. Sweeney R.W., Whitlock R.H., Rosenberger A.E. (1992): Mycobacterium paratuberculosis cultured from milk and supramammary lymph nodes of infected asymptomatic cows. Journal of Clinical Microbiology, 30, 166-171. Go to original source... Go to PubMed...
    111. Sweeney R.W., Whitlock R.H., Buckley C.L., Spencer, P., Rosenberger, A.E., Hutchinson, L.J. (1994): Diagnosis of paratuberculosis in dairy cattle, using enzyme-linked immunosorbent assay for detection of antibodies against Mycobacterium paratuberculosis in milk. American Journal of Veterinary Research, 55, 905-909. Go to original source...
    112. Tasara T., Stephan R. (2005): Development of an F57 sequence-based real-time PCR assay for detection of Mycobacterium avium subsp. paratuberculosis in milk. Applied and Environmental Microbiology, 71, 5957- 5968. Go to original source... Go to PubMed...
    113. Taylor T.K., Wilks C.R., McQueen D.S. (1981): Isolation of Mycobacterium paratuberculosis from the milk of a cow with Johne's disease. The Veterinary Record, 109, 532-533. Go to original source... Go to PubMed...
    114. Tunkle B., Aleraj Z. (1965): The recovering of M. johnei in the semen of bull from one station for artificial insemination. Veterinarski Glasnik, 19, 845-849.
    115. Wells S.J., Collins M.T., Faaberg K.S., Wees C., Tavornpanich S., Petrini K.R., Collins J.E., Cernicchiaro N., Whitlock R.H. (2006): Evaluation of a rapid fecal PCR test for detection of Mycobacterium avium subsp. paratuberculosis in dairy cattle. Clinical and Vaccine Immunology, 13, 1125-1130. Go to original source... Go to PubMed...
    116. Williams S.L., Harris N.B., Barletta R.G. (1999): Development of a firefly luciferase-based assay for determining antimicrobial susceptibility of Mycobacterium avium subsp. paratuberculosis. Journal of Clinical Microbiology, 37, 304-309. Go to original source... Go to PubMed...
    117. Act No.166/1999 Coll., on Veterinary Care and Amending Related acts (the Veterinary Act), as amended by Act No. 29/ 2000 Coll., Act No. 154/2000 Coll., Act No. 102/2001 Coll., Act No. 76/2002 Coll., Act No. 120/2002 Coll., Act No. 320/2002 Coll., Act No. 131/2003 Coll., Act No. 316/2004 Coll., Act No. 444/2005 Coll., and Act No. 48/2006 Coll. Collection of Acts of the Czech Republic, 2006, item 50, 70 pp.

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