Czech J. Food Sci., 2023, 41(1):29-35 | DOI: 10.17221/158/2022-CJFS

Screening and quantification of pesticide residues in ciders by liquid chromatography-high resolution mass spectrometryOriginal Paper

Veronika Zušťáková1,2, Martin Dušek2, Vladimíra Jandovská2, Jana Olšovská2
1 Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic
2 Research Institute of Brewing and Malting, Prague, Czech Republic

The present study aimed to apply a multi-residue method for the screening and quantification of pesticide residues in ciders, low alcoholic beverages made by fermentation of apple juice. Twenty bottled craft cider samples purchased from the Czech market were analysed for pesticide residues. The residues of pesticides were extracted from samples using the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) procedure in combination with additional solid-phase extraction (SPE) sample clean-up to achieve the lowest detection limit possible. In this study, targeted screening of pesticide residues in the samples was performed with the Q-Orbitrap mass spectrometry instrument. We identified 18 pesticides in cider samples analysed by screening method using an accurate-mass database of about 500 pesticide compounds, including their retention times, empirical formulas, and characteristic fragments. Additionally, liquid chromatography with high-resolution tandem mass spectrometer (LC-HRMS/MS) was used for re-analysis of positive findings of pesticides in samples and allowed to quantify compounds of interest at 0.2 µg L–1 concentration level. The monitoring scheme was applied to the set of craft ciders, and the results revealed the presence of pesticide residues in most of the samples at trace levels ranging from 0.5 to 5 µg L–1 and rarely at a level higher than 10 µg L–1.

Keywords: low alcoholic beverages; determination; apples; pesticide residues; Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS)

Accepted: January 11, 2023; Prepublished online: February 1, 2023; Published: February 27, 2023  Show citation

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Zušťáková V, Dušek M, Jandovská V, Olšovská J. Screening and quantification of pesticide residues in ciders by liquid chromatography-high resolution mass spectrometry. Czech J. Food Sci.. 2023;41(1):29-35. doi: 10.17221/158/2022-CJFS.
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