Czech J. Genet. Plant Breed., 2017, 53(1):30-36 | DOI: 10.17221/130/2015-CJGPB

Expression profiling of certain MADS-box genes in Arabidopsis thaliana plant treated with silver nanoparticlesOriginal Paper

Zainab Majed ALMUTAIRI*
Biology Department, College of Science and Humanities, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia

Silver nanoparticles (AgNPs) have been shown to enhance seed germination and plant growth. In this study, we examined the plant response to AgNP dosage using a model plant, Arabidopsis thaliana. Seedlings were treated with different concentrations of AgNPs. Seedling fresh and dry weights were measured for all treatments. The exposure of plants to 1 mg/l AgNPs resulted in a significant increase in seedling fresh and dry weights compared with control plants, although the exposure to lower and higher concentrations resulted in a decrease in fresh and dry weights. Expression profiling for 22 MADS-box genes was carried out using quantitative real-time PCR (qRT-PCR). Among the investigated MADS-box genes, eight genes were upregulated and four genes were downregulated at each of the AgNP concentrations, 0.1 and 1 mg/l. Revealing the effects of AgNPs on the expression of MADS-box genes will increase our knowledge of their role before implementing a large-scale agricultural utilization of AgNPs in the improvement of plant growth and development.

Keywords: AgNPs; molecular mechanisms; plant growth; qRT-PCR

Published: March 31, 2017  Show citation

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ALMUTAIRI ZM. Expression profiling of certain MADS-box genes in Arabidopsis thaliana plant treated with silver nanoparticles. Czech J. Genet. Plant Breed.. 2017;53(1):30-36. doi: 10.17221/130/2015-CJGPB.
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