Czech J. Anim. Sci., 2011, 56(9):390-397 | DOI: 10.17221/1291-CJAS
Proteolytic and anti-proteolytic activity in the seminal plasma of Eurasian perch (Perca fluviatilis L.) during the spawning period
- 1 Department of Ichthyology, University of Warmia and Mazury, Olsztyn, Poland
- 2 Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
The aim of this study was to describe possible changes in protease and anti-protease activity in the seminal plasma of European perch (Perca fluviatilis L.) during the spawning time. No significant difference in proteolytic activity was observed between the beginning and the late period of the spawning season in perch. Anti-protease activity significantly increased during the sampling time. Electrophoretic profiles of gelatinolytic activity in the seminal plasma of perch were characterized by four molecular forms, which depend on the presence of Ca2+ during incubation. We also found two forms of caseinolytic activity with low molecular weights, which were independent of calcium ions. However, both activities were fully stopped by the chelator of calcium ions (EDTA). In this study, non-typical profiles of gelatinolytic acti-vity were also observed. Profiles of protease activities in the perch seminal plasma are constant during the reproduction season indicating that the regulation of protease activity in seminal plasma occurs via protease inhibitors which are abundant in this fluid. Results concerning electrophoresis revealed at least seven forms of anti-proteases in the seminal plasma of perch. Concluded, anti-proteases comprise a high percentage of all proteins in the seminal plasma of perch, while the increase at the end of spawning season is probably protecting spermatozoa during the spawning time in perch testes.
Keywords: European perch; seminal plasma; protease and anti-protease activity
Published: September 30, 2011 Show citation
ACS | AIP | APA | ASA | Harvard | Chicago | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
References
- Alavi S.M.H., Rodina M., Policar T., Kozak P., Psenická M., Linhart O. (2007): Semen of Perca fluviatilis L.: Sperm volume and density, seminal plasma indices and effects of dilution ratio, ions and osmolality on sperm motility. Theriogenology, 68, 276-283. Go to original source... Go to PubMed...
- Alavi S.M.H., Rodina M., Hatef A., Stejskal V., Policar T., Hamáčková J., Linhart O. (2010): Sperm motility and monthly variations of semen characteristics in Perca fluviatilis (Teleostei: Percidae). Czech Journal of Animal Science, 55, 174-182. Go to original source...
- Bergmeyer H.U. (1985): Methods of enzymatic analysis. Weinheim, VCH Verlagsgesellschaft, 701 pp.
- Bowden T.J., Butler R., Bricknell I.R., Ellis A.E. (1997): Serum trypsin-inhibitory activity in five species of farmed fish. Fish and Shellfish Immunology, 7, 377-285. Go to original source...
- Ciereszko A., Dabrowski K., Feng Lin, Dorshov S.I. (1994): Identification of trypsin-like activity in sturgeon spermatozoa. Journal of Experimental Zoology, 268, 486-491. Go to original source...
- Ciereszko A., Dabrowski K., Ochkur S.I. (1996a): Characterization of acrosin-like activity of lake sturgeon (Acipenser fluvescens) spermatozoa. Molecular Reproduction and Development, 45, 72-77. Go to original source... Go to PubMed...
- Ciereszko A., Liu L., Dabrowski K. (1996b): Effects of season and dietary ascorbic acid on some biochemical characteristics of rainbow trout (Oncorhynchus mykiss) semen. Fish Physiology and Biochemistry, 15, 1-10. Go to original source... Go to PubMed...
- Ciereszko A., Glogowski J., Dabrowski K. (2000): Biochemical characteristics of seminal plasma and spermatozoa of freshwater fishes. In: Tiersch T.R., Mazik P.M. (eds.): Cryopreservation in Aquatic Species, World Aquaculture Society. Baton Rouge, Louisiana, 21-48.
- Ciereszko A., Wlasow T., Dobosz S., Goryczko K., Glogowski J. (2004): Blood cells in rainbow trout Oncorhynchus mykiss milt: Relation to milt collection method and sampling period. Theriogenology, 62, 1353-1364. Go to original source... Go to PubMed...
- Cosson M.P., Gagnon C. (1988): Protease inhibitors and substrates block motility and microtubule sliding of sea urchin and carp spermatozoa. Cell Motility and the Cytoskeleton, 10, 518-527. Go to original source...
- Dabrowski K., Ciereszko A. (1994): Protease inhibitor(s) in seminal plasma of teleost fish. Journal of Fish Biology, 45, 801-809. Go to original source...
- Inaba K., Akazome Y., Morisawa M. (1993): Purification of proteasomes from salmonid fish sperm and their localization along sperm flagella. Journal of Cell Science, 104, 907-915. Go to original source... Go to PubMed...
- Kestemont P., Melard C. (2000): Aquaculture. In: Craig J.F. (ed.): Percid Fishes: Systematics, Ecology and Exploitation. Blackwell Science, Oxford, 191-224. Go to original source...
- Kowalski R., Glogowski J., Kucharczyk D., Goryczko K., Dobosz S., Ciereszko A. (2003a): Proteolytic activity and electrophoretic profiles of proteases from seminal plasma of teleost. Journal of Fish Biology, 63, 1008-1019. Go to original source...
- Kowalski R., Wojtczak M., Glogowski J., Ciereszko A. (2003b): Gelatinolytic and anti-trypsin activities in seminal plasma of common carp: relationship to blond, skin mucus and spermatozoa. Aquatic Living Resources, 16, 438-444. Go to original source...
- Kowalski R., Glogowski J., Kucharczyk D., Mak M., Dobosz S., Zakes Z., Ciereszko A. (2004): Characterization of gelatinolytic activity in seminal plasma of some teleost fish. Aquaculture International, 12, 57-68. Go to original source...
- Krol J., Glogowski J., Demska-Zakes K., Hliwa P. (2006): Quality of semen and histological analysis of testes in Eurasian perch Perca fluviatilis L. during a spawning period. Czech Journal of Animal Science, 51, 220-226. Go to original source...
- Lahnsteiner F. (2007): Characterization of seminal plasma proteins stabilizing the sperm viability in rainbow trout (Oncorhynchus mykiss). Animal Reproduction Science, 97, 151-164. Go to original source... Go to PubMed...
- Lahnsteiner F., Berger B., Patzner R., Weismann T. (1995): Fine structure and motility of spermatozoa and composition of the seminal plasma in the perch. Journal of Fish Biology, 47, 492-508. Go to original source...
- Lahnsteiner F., Berger B., Patzner R., Weismann T. (1997): Sperm motility and seminal fluid composition in the burbot (Lota lota). Journal of Applied Ichthyology, 13, 113-119. Go to original source...
- Lahnsteiner F., Berger B., Patzner R., Weismann T. (1998): Determination of semen quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolism. Aqaculture, 163, 163-181. Go to original source...
- Lahnsteiner F., Mansour N., Berger B. (2004): Seminal plasma proteins prolong the viability of rainbow trout (Oncorhynchus mykiss) spermatozoa. Theriogenology, 62, 801-808. Go to original source... Go to PubMed...
- Li P., Hulak M., Linhart O. (2009): Sperm proteins in teleostean and chondrostean (sturgeon) fishes. Fish Physiology and Biochemistry, 35, 567-581. Go to original source... Go to PubMed...
- Loir M., Labbe C., Maisse G., Pinson A., Boulard G., Mourot B., Chambeyron F. (1990): Proteins of seminal fluid and spermatozoa of rainbow trout (Oncorhynchus mykiss): Partial characterization and variations. Fish Physiology and Biochemistry, 8, 485-495. Go to original source... Go to PubMed...
- Mochida K., Kondo T., Matsubara T., Adachi S., Yamauchi K. (1999): A high molecular weight glycoprotein in seminal plasma is a sperm immobilizing factor in the teleost Nile tilapia, Oreochromis niloticus. Development Growth and Differentiation, 41, 619-627. Go to original source... Go to PubMed...
- Mommens M., Wojtczak M., Ciereszko A., Babiak I. (2008): Seminal plasma proteins of Atlantic halibut (Hippoglossus hippoglossus L.). Fish Physiology and Biochemistry, 34, 349-355. Go to original source... Go to PubMed...
- Siegel M.S., Polakoski K.L. (1985): Evaluation of the human sperm proacrosin-acrosin system using gelatinsodium dodecyl sulfate-polyacrylamide gel electrophesis. Biology of Reproduction, 32, 713-720. Go to original source... Go to PubMed...
- Uriel J., Berges J. (1968): Characterization of natural inhibitors of trypsin and chymotrypsin by electrophoresis in acrylamide-agarose gels. Nature, 218, 578-580. Go to original source... Go to PubMed...
- Wilson J.M., Garcia B., Woodson M., Sinha A.A. (1992): Metalloprotease activities expressed during development and maturation of the rat prostatic complex and seminal vesicles. Biology of Reproduction, 47, 683-691. Go to original source... Go to PubMed...
- Wilson J.M., Norris H., Kapoor D., Woodson M., Limas C., Sinha A.A. (1993): Gelatinolytic and caseinolytic protease activities in the human prostatic secretions. Journal of Urology, 149, 653-658. Go to original source... Go to PubMed...
- Wlasow T., Glogowski J., Ciereszko A. (1999): Presence of blood cells in rainbow trout milt. Archives of Polish Fisheries, 7, 359-364.
- Wojtczak M., Dietrich G.J., Ciereszko A. (2005): Transferrin and antiproteases are major proteins of common carp seminal plasma. Fish and Shellfish Immunology, 19, 387-391. Go to original source... Go to PubMed...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.