Czech J. Anim. Sci., 2012, 57(8):345-352 | DOI: 10.17221/6269-CJAS

Genetic variation in three paddlefish (Polyodon spathula Walbaum) stocks based on microsatellite DNA analysis

D. Kaczmarczyk, M. Luczynski, P. Brzuzan
Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Poland

There are two stocks of American paddlefish Polyodon spathula in Poland, one in Pogorze and the other in Wasosze. These stocks were established from small quantities of eggs imported from the USA in 1995. In this study, we examined genetic variation at seven microsatellite loci in adult fish from the two Polish farms and one stock farmed in Gorny Tykich in Ukraine. Our data were compared with those reported for one native population from the Mississippi River (USA). The polymorphism of examined loci varied in the Polish and Ukrainian stocks, showing 25-30 alleles across each stock (3.6-4.3 alleles per locus), though they were less polymorphic than those in the Mississippi (49 across the population, 7.0 per locus). The mean observed heterozygosity per locus estimated in Polish stocks (0.59-0.60) was comparable to that found in the Mississippi (0.68). The Garza-Williamson index and values of the heterozygosity excesses revealed a reduction of genetic variation in all the three European stocks (probably resulting from bottleneck or founder effect). Finally, genetic distance measurements confirmed a closer relationship between Pogorze and Wasosze (FST = 0.007) stocks than between these two Polish stocks and that from Ukraine (FST = 0.096 and FST = 0.054).

Keywords: broodstocks; aquaculture; founder effect; genetic distance; genetic variation

Published: August 31, 2012  Show citation

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Kaczmarczyk D, Luczynski M, Brzuzan P. Genetic variation in three paddlefish (Polyodon spathula Walbaum) stocks based on microsatellite DNA analysis. Czech J. Anim. Sci.. 2012;57(8):345-352. doi: 10.17221/6269-CJAS.
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