Plant Protect. Sci., 2016, 52(1):10-17 | DOI: 10.17221/34/2015-PPS

Antifungal activities of Chaetomium spp. against Fusarium wilt of teaOriginal Paper

Nguyen Huu Phong^{1, 2}, Wattanachai Pongnak¹, Kasem Soytong¹

¹ Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand

² Northern Mountainous Agriculture and Forestry Science Institute, Phu Tho, Vietnam

An isolate of Fusarium denominated as NHP-Fusa-2 from tea wilt and root-rot diseased sample collected in Vietnam was identified as Fusarium oxysporum based on molecular analysis of translation elongation factor-1α sequence. Interestingly, it is the first time F. oxysporum is reported as a causal pathogen of wilt and root-rot disease of tea in Vietnam. Chaetomium spp. were investigated to control F. oxysporum NHP-Fusa-2 in in vitro test. Three antagonists (Ch. cupreum CC3003, Ch. globosum CG05, and Ch. lucknowense CL01) inhibited mycelial growth by 31.69-34.03% and reduced conidial production of the pathogen by 67.25-75.92% in the bi-culture antagonistic test after 30 days with non-significant difference in their effect. All the crude extracts of these antagonists significantly inhibited mycelial growth and conidial production of NHP-Fusa-2. MeOH extract of CC3003 was more effective in conidial inhibition of the pathogen than the others, the effective dose (ED₅₀) was 85.30 μg/ml of which, it was hexane (49.32 μg/ml) and EtOAc extract (62.17 μg/ml) in the case of CG05 and CL01, respectively.

Keywords: Chaetomium species; Fusarium oxysporum; growth; sporulation; inhibition

Published: March 31, 2016  Show citation