Czech J. Food Sci., 2016, 34(3):189-196 | DOI: 10.17221/25/2016-CJFS

Microbiological quality of raw milk in the Czech RepublicFood Microbiology and Safety

Kateřina Bogdanovičová1, Marcela Vyletělová-Klimešová2, Vladimír Babák3, Libor Kalhotka4, Ivana Koláčková3, Renáta Karpíšková3
1 Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
2 Dairy Research Institute, Prague, Czech Republic
3 Veterinary Research Institute, Brno, Czech Republic
4 Faculty of Horticulture, Mendel University in Brno, Brno, Czech Republic$3

The microbiological and hygienic quality of cow's, goat's and sheep's milk in the Czech Republic was evaluated. Milk (230 samples) was collected on 41 farms and investigated from May 2012 to October 2014. Milk was analysed for the presence of selected groups and types of bacteria: mesophilic microorganisms (total plate count - TPC), enterococci, Enterobacteriaceae, Escherichia coli, Staphylococcus aureus, Salmonella spp., Campylobacter spp., and Listeria monocytogenes. Besides these indicators and pathogenic agents, somatic cell count was determined as one of the indicators of mammary gland health in cows. TPC ranged between 8.3 × 102 and 1.2 × 109 CFU/ml and somatic cells between 1.6 × 104 and 6.8 × 106 cells/ml. The presence of E. coli was confirmed in 86.3% of samples and the colony counts ranged from 1.0 × 101 to 4.0 × 106 CFU/ml. The presence of verotoxigenic E. coli was confirmed in 3 samples (1.3%) (cow's milk 0%; goat's milk 6.3%; sheep's milk 4.4%). The presence of S. aureus was confirmed in 29.1% of the samples (cow's milk 26.9%; goat's milk 34.4%; sheep's milk 39.1%), but the numbers were very low (< 5.0 × 102 CFU/ml). L. monocytogenes was confirmed in 3 examined samples (1.3%) (cow's milk 0.6%; goat's milk 3.1%; sheep's milk 4.4%). Salmonella spp. and Campylobacter spp. were not detected in any of the samples tested.

Keywords: raw milk; TPC; SCC; enterococci; Enterobacteriaceae; Escherichia coli; Staphylococcus aureus; Salmonella spp.; Campylobacter spp.; Listeria monocytogenes

Published: June 30, 2016  Show citation

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Bogdanovičová K, Vyletělová-Klimešová M, Babák V, Kalhotka L, Koláčková I, Karpíšková R. Microbiological quality of raw milk in the Czech Republic. Czech J. Food Sci.. 2016;34(3):189-196. doi: 10.17221/25/2016-CJFS.
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