General Characteristics and Treatment Possibilities of Dairy Wastewater – A Review

The dairy industry includes the transformation of raw milk into pasteurised and sour milk, yoghurt, hard, soft and cott age cheese, cream and butt er products, ice cream, milk and whey powders, lactose, condensed milk, as well as various types of desserts (1–6). The general distinctions among these foods are due to the reuse of non-fat milk and whey (a by-product in cheese manufacturing) and the evaporation of the free water from the coagulum as well as from milk and whey powders (5). With the rapid industrialisation observed in the last century (4) and the growing rate of milk production (around 2.8 % per annum), dairy processing is usually considered the largest industrial food wastewater source, especially in Europe (1–3,7). Moreover, in around 50 % of the world’s whey production, especially concerning acid whey, it is untreated prior to disposal (8–10). The effl uents originating from various production technologies are not discharged simultaneously, thus forming a stream with wide qualitative and quantitative variations (4). Notwithstanding the diff erences in composition, att ributable to the manufactured product and technological operations (11,12), dairy effl uents are distinguished by their relatively increased temperature, high organic content (13–15) and a wide pH range, which requires special purifi cation in order to eliminate or reduce environmental damage (1). Treatments of dairy wastewaters include the application of mechanical, physicochemical and biological methods. Mechanical treatment is necessary to equalise volumetric and mass fl ow changes. It also reduces parts of the suspended solids. Physicochemical processes are eff ective in the removal of emulsifi ed compounds, but reagent addition increases water treatment costs. Another disadvantage is the very low elimination of soluble chemical oxygen demand (COD). Therefore, biological wastewater treatment systems are preferred due to the highly biodegradable contaminants (7,11,16,17).


Introduction
The dairy industry includes the transformation of raw milk into pasteurised and sour milk, yoghurt, hard, soft and cott age cheese, cream and butt er products, ice cream, milk and whey powders, lactose, condensed milk, as well as various types of desserts (1)(2)(3)(4)(5)(6).The general distinctions among these foods are due to the reuse of non--fat milk and whey (a by-product in cheese manufacturing) and the evaporation of the free water from the coagulum as well as from milk and whey powders (5).With the rapid industrialisation observed in the last century (4) and the growing rate of milk production (around 2.8 % per annum), dairy processing is usually considered the largest industrial food wastewater source, especially in Europe (1)(2)(3)7).Moreover, in around 50 % of the world's whey production, especially concerning acid whey, it is untreated prior to disposal (8)(9)(10).The effl uents originating from various production technologies are not discharged simultaneously, thus forming a stream with wide qualitative and quantitative variations (4).Notwithstanding the diff erences in composition, att ributable to the manufactured product and technological operations (11,12), dairy effl uents are distinguished by their relatively increased temperature, high organic content (13)(14)(15) and a wide pH range, which requires special purifi cation in order to eliminate or reduce environmental damage (1).Treatments of dairy wastewaters include the application of mechanical, physicochemical and biological methods.Mechanical treatment is necessary to equalise volumetric and mass fl ow changes.It also reduces parts of the suspended solids.Physicochemical processes are eff ective in the removal of emulsifi ed compounds, but reagent addition increases water treatment costs.Another disadvantage is the very low elimination of soluble chemical oxygen demand (COD).Therefore, biological wastewater treatment systems are preferred due to the highly biodegradable contaminants (7,11,16,17).
The purpose of the paper is to review the data on the basic composition and treatment possibilities of milk--processing effl uents.Their origin and major characteristics are summarised.Various methods for wastewater utili sation are discussed.Finally, suggestions for future research are made.

Wastewater volume
Water plays a key role in milk processing.It is used in every step of the technological lines, including cleaning and washing, disinfection, heating and cooling.Water requirements are huge (14).
The bulk of wastewater comes from manufacturing processes (6).Contaminated water, including sanitary activities, reaches 50-80 % of the total water consumed in the dairy factory, whereas the remaining 20-50 % is conditionally clean (6,18).It has been estimated that the amount of wastewater is approx.2.5 times higher than that of processed milk in units of volume.The amount and characteristics of the wastewater depend largely on the factory size, applied technology, eff ectiveness and complexity of clean-in-place (CIP) methods, good manufacture practices (GMP), etc. (3,4).However, the introduction of GMP can reduce the world's wastewater mean volume from 0.5-37 to 0.5-2 m 3 of effl uent per m 3 of processed milk (3,19).Nowadays, the designed volumetric load is 1 m 3 of effl uent per tonne of manufactured milk (4).
In dairy plants, the great fl uctuations in wastewater quality and quantity are very problematic because each milk product needs a separate technological line (1,5).This results in the change of dairy effl uent composition with the start of a new cycle in the manufacturing process, which impedes the work of in-factory wastewater treatment plants.Furthermore, intensive effl uent volumetric variations in time are commonly observed.Daily and hourly changes are the consequence of washing the equipment and fl oors as the fi nal step in every process cycle.Seasonal variations can be att ributed to a higher dairy plant load in summer than in winter (20).One way of explaining hourly homogeneity is by coeffi cients in the range of 1.4-2.0(6).The diurnal inequality coeffi cient depends on the seasonal character of dairy processing, varying from 1.5 for 2-and 3-shift work in summer to 2.6 for winter shift s.The actual concentration of polluting dairy effl uents varies widely depending on the profi le and capacity of the company, the production technology, the type of equipment used, the degree of wastewater reuse, the loss of raw materials, waste management, etc. (1,18).A major factor in the volumetric loading of dairy wastewater treatment plants are the immediate discharges produced in the cleaning of tank trucks, pipelines or equipment at the end of each cycle.In such cases, the effl uent volumes are higher than those of manufactured milk (4,21).On average, wastewater discharge is 70 % of the amount of the fresh water used at the plant (6).
Dairy processing effl uents mostly include milk or milk products lost in the technological cycles (spilled milk, spoiled milk, skimmed milk and curd pieces); starter cultures used in manufacturing; by-products of processing operations (whey, milk and whey permeates); contaminants from the washing of milk trucks, tanks, cans, equipment, bott les and fl oors; reagents applied in CIP procedures, cooling of milk and milk products, for sanitary needs, in equipment damage or operational problems; and various additives introduced in manufacturing (13,18,22,23).Milk loss in wastewater is around 0.5-2.5 % of processed milk, but it can increase to 3-4 % (20).

Wastewater categories
The wide variety of dairy products presupposes the existence of many wastewater types.However, three major categories can be outlined according to their origin and composition (1), explained in the following chapters.

Processing water
Processing water is formed in the cooling of milk in special coolers and condensers, as well as condensates from the evaporation of milk or whey.Milk and whey drying produces vapours which form the cleanest effl uent aft er condensation although it may contain volatile substances as well as milk or whey droplets from evaporators (6).In general, processing waters lack pollutants and, aft er minimal pretreatment, they can be reused or discharged together with stormwater (1).Water reusage is possible for installations that are not in direct contact with derived products.Typical applications include hot water and steam production as well as membrane cleaning.The water from the cooling of products during pasteurisation aft er the last rinse of bott les and condensates generated in vacuum installations from secondary vapours can be utilised for room cleaning, lawn irrigation, etc. (6,18).

Cleaning wastewater
Cleaning wastewater usually comes from washing equipment which is in direct contact with milk or dairy products.It also includes milk and product spillage, whey, pressing and brine, CIP effl uents or equipment malfunction and even operational errors.Over 90 % of organic solids in effl uents come from milk and manufacturing residues: cheese pieces, whey, cream, water from separation and clarifi cation, starter cultures, yoghurt, fruit concentrates or stabilisers.These effl uents are in large quantities and are highly polluted, thus requiring further treatment.

Sanitary wastewater
Sanitary wastewater is found in lavatories, shower rooms, etc. Sanitary wastewater is similar in composition to municipal wastewater and is generally piped directly to sewage works (1,6,21).It can be used as nitrogen source for unbalanced dairy effl uents before a secondary aerobic treatment (1,12).
Additionally, the by-products of manufacturing processes, such as whey, milk and whey permeates, can also be grouped separately if they are collected individually from other wastewater streams (12,24,25).
The main pollutant in milk processing wastewater is whey due to its high organic and volumetric load.It represents about 85-95 % of the milk volume and 55 % of the milk components.Whey consists of carbohydrates (4-5 %), mostly lactose.Proteins and lactic acid amount to less than 1 %, fats to around 0.4-0.5 %, while salts vary from 1 to 3 % (2,15,26).Whey is produced mainly in cheese manufacturing, and its volume depends on the productivity of cheese and the type of processed milk -bovine, goat, sheep, etc. (2,9,27).On the basis of milk casein coagulation procedures, whey can be categorised as cheese whey and second cheese whey.Cheese whey is a by-product in the production of hard, semi-hard and soft cheese, aft er the addition of rennin to milk.Mild enzyme action produces sweet whey with a pH=6-7 (2,28).Second cheese whey is a by-product in cott age cheese production aft er milk has been fermented, or curdled, with organic or mineral acids.Due to strong acid conditions, whey develops an acidic taste, while the average pH value rarely exceeds 5. Scientifi c literature also discusses casein whey whose composition is very close to that of second cheese whey (2).Sweet and acid whey also diff er in mineral and protein content (9,29).
During cheese manufacturing, cheese whey wastewater is produced as well.Its volume and composition change with respect to the type of produced cheese, the applied technology, the milk type and the environment.Cheese whey wastewater originates in the addition of surplus cheese whey and second cheese whey to washing effl uents.Nevertheless, its contamination level is lower than that of cheese whey (2,30).
Cheese whey waste streams are valuable sources of diff erent compounds (protein, lactose, mineral elements) and are utilised in the manufacture of various products, such as lactic acid, single-cell protein, baker's yeast, starter cultures, fermented whey drinks, enzymes, antibiotics, organic acids, vitamins, food gums, etc.Nevertheless, it should be taken into account that whey or whey product recovery results in new waste streams which also need to be treated although such effl uents are less polluted than whey and their organic loading is comparable to other dairy wastewater (4,9,31,32).
Milk and whey permeates are by-products in cheese manufacturing; they are produced during milk and whey ultrafi ltration, respectively.Their solid content is lower; they are rich in soluble compounds, over 80 % of which is lactose (24,25,33).
Dairy wastewater consists of complex constituents (11,34).Knowing the composition of milk and milk products, we can estimate bett er the wastewater contaminant loading (Table 1) (22).Although milk manufacturing produces waste streams analogous to milk and dairy product loss, every process gives an effl uent unique in volume and composition (4).
Dairy wastewater volumetric and fl ow rates (depending on the production capacity and work shift s), as well as pH and total suspended solids (TSS) content (as a consequence of applied CIP methods) aff ect the effi ciency of wastewater treatment management (1).It is important to know the quantity of the milk to be pasteurised, how much milk is processed into cheese and whether the entire obtained whey is discharged in wastewater or part of it is processed and reused (6).Contaminant concentrations in wastewater can be determined by using Eq.1: where C is contaminant concentration in wastewater (g/ m 3 ), L is the loss of milk and milk products in diff erent technological production cycles expressed in proportional units (m 3 or t), C 1 , C 2 and C n are contaminant concentration per unit of milk or milk product loss (g/t), and N 1 , N 2 and N n are wastewater discharge per unit of milk or generated milk products (m 3 /t) (18).
Typically, dairy wastewater is white in colour (whey is yellowish-green) and has an unpleasant odour and turbid character (2,12,36).
With annual temperatures of 17-25 °C, dairy waste streams are warmer than municipal wastewater (10-20 °C), which results in faster biological degradation compared to sewage treatment plants (37).The average temperatures of industrial dairy effl uents range from 17-18 °C in winter and 22-25 °C in summer (6).Using the Arrhenius equation, the biodegradation rates and oxygen consumption can be predicted to be 1.5 times higher in summer than in winter (37).The design winter temperature of 15 °C is adopted for this type of wastewater due to the utilisation of hot water for washing and cleaning of equipment (6,18).
Cott age cheese whey Cheese whey wastewater BOD A crucial requirement for biological treatment of dairy wastewater is their pH value between 6 and 9 (37).Milk and butt er factories have effl uents with active reaction close to neutral (pH=6.8-7.4).In plants where a certain amount of whey is discharged, the pH of the effl uent is reduced to below 6.2.In cheese manufacturing, sweet whey is slightly acidic, with pH=5.9-6.6, while mineral acid coagulation gives an acidic whey with pH=4.3-4.6 (6,27).The sharp increase in the short-term pH of the total fl ow of up to 10-10.5 is att ributable to the discharge of alkaline cleaning solutions.The prolonged exposure of wastewater to anaerobic conditions (in the sewer network with sumps) causes liquid acidifi cation by lactic acid fermentation that leads to a decrease in pH (18).
Although dairy wastewaters have low concentrations of sett leable solids, they may clog sewage pipes.Most of the suspension enters the initial stage of equipment cleaning.The bulk of the sediment (90 %) of organic matt er is usually of protein origin, namely particles of solid milk processing (pieces of cheese, coagulated milk, cheese, curd fi nes, milk fi lm or fl avouring agents, etc.) and other impurities (soil or sand) that get into the sewage system during equipment washing or packaging (12,18).Formation of protein and fat deposits on the inside of the pipes requires periodic cleaning with appropriate chemical or bacterial preparations.The main advantage in the application of such bacteria is that they continue acting in the next stages of wastewater treatment, increasing the purifi cation effect (38).The highest amount of total solids (TS) has been reported in whey, with negligible amount of volatiles (3).Fats in dairy industry effl uents are found in trace amounts in the form of emulsions with a droplet diameter of 1-10 μm (6).During homogenisation, the size of milk fat globules is reduced to 1-2 μm.The obtained stable emulsion, when passing into dairy effl uents, aff ects the mechanical wastewater treatment system due to its diffi cult separation (4).Thus, fats remaining in cheese whey wastewater can produce an undesired fl otation, which results in the washout of active sludge during biological processes (2).In the production of high-fat products (cream, sour cream and butt er), larger fat globules are extracted from the milk, due to their coalescence and enlargement, as well as the degradation of the protein shell.That is why fat impurities in the wastewater from these productions are signifi cantly diff erent in type and concentration and their elimination by sett ling is more effi cient than in other dairy effl uents.The FOG concentration in the wastewater from dairy plants specialised in the production of high-fat products is 0.2-0.4g/L although higher values (up to 2.88 g/L in a butt er factory have been reported) (18).In the wastewater from other dairy plants, it usually does not ex ceed 0.1 g/L (18).
Due to their high organic content, represented mainly by rapidly assimilable carbohydrates and slowly degradable proteins (20) and lipids, dairy wastewater is characterised by high BOD and COD values varying from 0.1 to 100 g/L (3,11,27,39).It is known that there is a direct relationship between the ultimate 20-day BOD (BOD u ) and COD values in dairy wastewater, as shown in Eq. 2: It should be taken into account that such a logical connection cannot be made between a 5-day BOD (BOD 5 ) and BOD u , and between BOD 5 and COD.Therefore, BOD 5 value of dairy waste streams is not an objective indicator of organic pollution (18).Nevertheless, many authors use the BOD 5 value of dairy wastewater in the BOD 5 /COD ratio.For dairy effl uents this ratio varies between 0.4 and 0.8 (10,12,20,24).However, it should be determined separately in every particular case (40) and, since dairy wastewater is industrial, the BOD analysis should be conducted with selected microbial consortia, instead of traditional seeding material in order to achieve reliable results (41).
The highest whey COD and BOD 5 concentrations have been reported to be between 60-80 and 30-50 g/L, respectively.About 90 % of BOD and COD loading is caused by lactose, while protein removal contributes to only around 12 % of the whey COD reduction.High lactose solubility increases soluble COD part, which is removed mostly by biological units.Like whey, milk and whey permeates have high COD load because they are rich in lactose, which excludes the possibility for a direct discharge in water bodies (15,24).Cheese whey wastewater also has increased concentrations of organic matt er, the values varying signifi cantly: 0.8-77 g/L of COD and 0.6-16 g/L of BOD 5 .The lower lactose concentration reported is due to the fermentation in anaerobic conditions that leads to a lower initial pH and casein precipitation and odour production from the obtained butyric acid (2).
The time-consuming BOD analysis requires the application of faster methods that determine aerobically digestible organic matt er in dairy wastewater.Many authors show that COD fractionation combined with the calculation of respirometric oxygen uptake rate is a good alternative method for the determination of wastewater biodegradability (42)(43)(44).However, results were obtained only for mixed dairy wastewater (45)(46)(47), while the information on single manufacturing processes is insuffi cient (48).Total organic carbon (TOC) calculation also includes organic carbonaceous fractions.It gives immediate results and can be used for online measurements.However, the TOC-BOD relationship should be estimated fi rst (49).There is no available scientifi c data for the online TOC application or the TOC-BOD relationship in dairy wastewater treatment.
Every milk effl uent has notably diff erent TN and TP concentrations (3).Nitrogen exists mainly in the form of amino groups from milk proteins.Other nitrogenous compounds are also detected: urea, uric acids, and NH 4 + , NO 2 ¯ and NO 3 ¯ ions (11,27).Small quantities of nitrogen ammonium salts originating from ammonia compressors can also be found (18).Phosphorus compounds are mostly inorganic, phosphate (PO 4 ), but they can also be present in organic form (11). Total nitrogen content in the wastewater from urban dairies, dairy and butt er plants is 4.2-6 % and that from cheese factories 3.7 % of the BOD 5 .The phosphorus concentration is in the 0.6-0.7 % range of the BOD 5 .The reported TN and TP values demonstrate an increased eutrophication risk in water receivers.Their concentrations are sufficient for normal biological treatment processes and the respective growth of bacteria involved in the oxidation of dairy wastewater impurities.However, cheese effl uents lack in nitrogen for proper aerobic biological treatment due to the following C/N/P ratio of approx.200:3.5:1but can easily be treated anaerobically (50).During biological treatment of cheese factory wastewater, nitrifi cation is less intense than in other dairy industry waste water treatment facilities because of the lower BOD 5 /N ratio (2,12,18).Dairy effl uents are characterised by very low alkalinity (approx.2.5 g/L expressed as CaCO 3 in milk permeate), thus bringing about a potential for rapid acidification and increased reagent costs for pH maintenance during purifi cation (15,24).
The high salinity of industrial dairy effl uents causes a non-volatile suspended solid content increase in the primary and secondary sludge.Inorganic impurities in dairy wastewater are represented by Na + , K + , Ca 2+ and Cl¯ ions, with their highest amounts in cheese and cott age cheese production (0.46-10 %), mostly NaCl and KCl (>50 %) as well as Ca 3 (PO 4 ) 2 , where salt is added in advance.Increased Na + amounts indicate the application of alkaline cleaning agents in milk factories.The amount of Ca 2+ in acidic whey is twice as high as that in sweet whey (2,12).The presence of chlorides in dairy wastewater is due to the addition of salt in the production of brine and cooling liquors, and the Cl¯ concentration in fresh water and milk.Cl¯ concentration in dairy wastewater reaches 0.8-1 g/L but the average value range is 0.15-0.2g/L.
The additional wastewater pollution due to the used cleaning solutions, additives and other products which enter the drainage pipes should be taken into account (4,18).CIP methods produce wastewater streams at 12-or 24-hour time intervals, while sanitisers are used if the dairy factory has been shut down for more than 96 h.Thus, wastewater pH will change widely depending on the cleaning program applied (23).Diff erent chemical solutions can be used in accordance with the installation type, water hardness, etc. (4).The cleaning agents applied in CIP procedures aff ect principally the effl uent pH (mineral and organic acids), contributing less than 10 % to BOD 5 and COD loading and increasing amounts of water for cleaning and disinfection (up to 30 % of total water fl ow rate).Most of the applied chemicals are very toxic to microorganisms in secondary treatment units.NaOH and HCl increase the mineral scale (1), while HNO 3 , quaternary ammonium surfactants, and detergents containing H 3 PO 4 and P infl uence TN and TP loading, which leads to an accelerated eutrophication of the environment if not treated properly (6).Due to the above-mentioned environmental problems, the trend is to apply more HNO 3 instead of the less desirable H 3 PO 4 although the latt er is a bett er cleaner the application of which will not be reduced in the future.The cleaning solutions utilised in CIP procedures are hot (64-82 °C), which causes a temperature increase in the resulting effl uents (4).Strong oxidants or bleaches (NaOCl and ClO 2 ) are applied for sanitising installations.Cl-containing bleaching agents can produce dangerous organochlorides which pollute dairy effl uents.Enzymes as well as surfactants are the chemicals preferred for cool surface cleaning and cause fewer negative environmental problems (6).In minor doses, the following substances can also be found: NH 3 , Na 3 PO 4 , HCl, HO-CH 2 COOH, Na 2 SiO 3 , hydraulic oil, propylene glycol, emulsifi ers, antifoaming agents, sodium azide and chloramphenicol (4,51).

Dairy Wastewater Treatment
Dairy manufacturing has a strong impact on the environment, producing large volumes of wastewater with high organic and nutrient loading and extreme pH variations.This requires the application of eff ective and cheap wastewater treatment procedures which ensure fresh water preservation (1,4).There are various dairy effl uent treatment strategies (Fig. 1), which are described in the following paragraphs.

Discharge in nature without treatment
It is not recommended that raw dairy wastewater be discharged directly into water bodies because this would lead to diff erent pollution problems, including rapid dissolved O 2 depletion due to the high organic loading, which results in anaerobic conditions, the release of volatile toxic substances, aquatic life destruction and subsequent environmental damage.Higher water temperatures decrease O 2 solubility and increase biota sensitivity (1).

Treatment in wetlands
Wetland systems use natural processes that include self-supported microbial communities to improve wastewater treatment (52).The simple construction and the lack of sludge recycling make them preferable for dairy effl uent utilisation in developing communities (52,53).The main drawbacks of their application include the need for a large surface area, the potential risks for surface and ground water pollution, the presence of dangerous volatile substances and the presence of insects.The easy exploitation of the systems counteracts with the complexity of the biological processes, which exceeds that of other treatment systems applied in wastewater purifi cation.Also problematic is the generation of Fe 3+ , Mn 3+ and Ca 2+ ions.They precipitate and reduce bed permeability with time.As a result, anaerobic conditions prevail and the NH 3 removal is limited (1).
Generally, dairy wastewater is treated in wetlands under aerobic conditions.Five days are enough for an 85 % BOD 5 reduction in aerobic ponds with milk wastes at 20 °C, while high-load dairy wastewater is treated mostly in facultative wetlands (1).In a butyl-covered lagoon, processing effl uent was biodegraded at 35 °C, with the organic loading rate (OLR), expressed as COD, of 1.5 kg/ (m 3 •day), neutral pH and a hydraulic retention time (HRT) of 1-2 days.However, a polishing step in aerated pond is necessary to achieve 99 % total COD reduction (1,4).A surface-fl ow wetland was applied to utilise 2.65 m 3 /day of milkhouse wastewater with OLR, expressed as BOD 5 , of 7.3 g/(m 2 •day) (54).The results showed high TSS, BOD 5 , TP and total Kjeldahl nitrogen (TKN) biodegradation with respective values of 94, 85, 68 and 53 %.Despite the fact that the lagoon produced NH 3 , its outfl ow concentrations gradually declined over time.Most of the nitrogen was stored in biomass, while denitrifi cation had a minor role (<1 %) (54).Clarifi ed effl uents needed more BOD 5 reduction to meet water discharge standards (55).Cheese wastewater with OLR, expressed as COD, of 5.5 kg/(m 3 •day) was consistently treated in a grease trap, an upfl ow anaerobic sludge blanket (UASB) reactor-type pond, aerobic pond and a fi nal wetland with water hyacinth (56).A high quality effl uent was obtained: BOD 5 , COD, TSS, FOG, organic N and total coliforms were reduced by more than 90 %, except for the phosphorus from PO 4 3-(with a decrease of only 62 %).

Purifi cation in urban or in-factory wastewater treatment plant
In-plant effl uent treatment is the most common strategy for dairy wastewater purifi cation (1).Typically, it includes mechanical, physicochemical, chemical and biological methods.

Mechanical treatment
Mechanical treatment removes suspended solids from wastewater.Conventional mechanical procedures reduce insuffi ciently the organic load because of the low sett leable solid concentration in dairy wastewater (5).Nevertheless, the faster the wastewater is screened, the bett er, due to less TSS biodegradation and a low soluble COD increase (1).
High variations of dairy effl uents can bring about an instability of the subsequent treatment facilities.Adequate equalisation will smooth the fl uctuations in the fl ow, organic loading, pH and temperature, neutralise residual cleaning agents and completely destroy excess oxidisers.In practice, a 24-hour fl ow patt ern at the highest load can be eff ectively handled by effl uent equalisation for at least 6-12 h with a basin dimension from 25 to 50 % of the total effl uent volume (1,6).

Physicochemical treatment
Physicochemical treatment destroys and reduces milk fat and protein colloids in the dairy wastewater (4).FOG removal is a major problem in the plants producing unskimmed milk, in milk and whey separation, cheese and butt er production, as well as milk bott ling.Skimmed milk production rarely creates such problems.
Animal fat is solid at room temperature due to the high levels of saturated fatt y acids in its composition.Milk fat is no exception.This physical state, combined with the low density of the fat allows its easy removal from the surface of wastewater (6,57).If the equalisation unit precedes the FOG trap, a temperature drop will heighten the risk of high fat accumulation on the top of the liquid.Otherwise, the equalisation unit must have a suffi cient volume to collect the peak effl uent fl ow.In general, fl ow balancing is followed by FOG removal.Increased wastewater temperatures can reduce fat separation ability (1,5).Dissolved air fl otation is more eff ective because it reduces organic loading via protein and fat colloid destabilisation with coagulants (Al 2 (SO 4 ) 3 , FeCl 3 and FeSO 4 ) and fl occulants.Nevertheless, this method requires expensive, synthetic chemicals which causes environmental problems and removes soluble matt er to a lesser extent (58).The resulting scum is very hard to dewater and it is not recommended to mix it with activated sludge.Scum must be treated properly before disposal (1,4).If inorganic and synthetic chemicals are replaced by biopolymers (carboxymethyl cellulose (CMC) or chitosan), processed sludge can be used as an animal food ingredient (59,60).
According to some authors (60-62) natural coagulation in dairy wastewater can be achieved with the application of certain lactic acid bacteria.These bacteria ferment soluble lactose to lactic acid, which denatures milk proteins in the wastewater.In combination with CMC, the total COD was reduced by 65-78 %, while reduction of 49-82 % was obtained when chitosan was used (60).At an initial 5 g/L of COD, over 0.01 g/L of proteins and 0.7-0.8g/L of sugars, 75, >90 and 10-25 % of COD were removed, respectively (61).

Chemical treatment
Chemical treatment removes mostly colloids and soluble contaminants from milk processing effl uents.It in-cludes reagent oxidation or pH correction.During cheese wastewater reaction with FeSO 4 and H 2 O 2 , up to 80 % of fat (initial concentration of 1.93 g/L) is removed (63).Extreme pH values of dairy wastewater below 6.5 and above 10 can increase the corrosion of pipes and be highly detrimental to microbiological assemblages in biological processes.Therefore, they should be corrected to reduce side eff ects.If a dissolved air fl otation (DAF) unit is used, then the pH control is a necessary step to achieve optimal coagulant conditions (64).However, coagulants work best at an acidic pH, which requires a second pH adjustment to a neutral value before biological treatment (65).It is very suitable to collect independently used CIP solutions and outfl ow them constantly during the whole wastewater plant exploitation (1,4).

Biological treatment
One of the most reliable methods for dairy effl uent purifi cation is biological removal.Such methods can assimilate all dairy wastewater components but they mostly utilise soluble compounds and small colloids.These processes have not been fully studied.Moreover, because of their unlimited adaptation potential, they can be jointly used in various sequences to meet certain component biodegradation requirements (1,7).Biological treatment has two main branches depending on oxygen requirements: aerobic and anaerobic processes (66).Aerobic processes.Nowadays, most dairy wastewater treatment plants are aerobic although they have been less effi cient, mainly due to fi lamentous growth and rapid acidifi cation caused by high lactose levels and low water buff er capacity, respectively (4,12,67).Problems generally encountered with activated sludge processes are bulking and foaming, which diminish sludge sett ling, Fe 3+ and CO 3 2precipitation, additional biomass production as well as poor activity at low temperatures.It takes a few months for the sludge adaptation before full operational capacity is reached.Nitrogen from NH 3 is easily degraded.Phosphorus removal is less eff ective and relies on environmental conditions.Aerobic bacteria are less useful in colloid utilisation when compared to anaerobic bacteria.The heightened O 2 depletion (>3 kg of O 2 per kg of BOD 5 ) requires large energy demands during the aerobic treatment of concentrated dairy wastewater (>2 g of COD per L) (1,4).Plug fl ow systems are bett er than complete-mix processes since they are less sensitive to high organic load problems like bulking sludge, etc. (21).Commonly, dairy effl uent OLR, expressed as BOD 5 , should be less than 0.28-0.30kg/m 3 .To enhance biological removal, a proper pretreatment or adequate wastewater dilution should be applied (1,68).
Aerobic biological systems give a very positive response during synthetic dairy wastewater treatment with 4 g/L of COD and 1 g/L of TKN at pH=11.5, with over 96 % of degradation being achieved in a continuous mode (69).An artifi cial effl uent similar to milk powder and butter processing wastewater was treated in an anaerobic--anoxic-oxic system at HRT of 7 days and a nominal sludge age of 20 days (70).The process was characterised by sludge bulking due to the growth of fi lamentous bacteria (Sphaerotilus natans, Type 0411 and Haliscomenobacter hydrossis).TN removal remained unchanged at 66 % with-out the improvement in the sludge volume index.TP depended on the anoxic selector relative dimensions (from 49 to 20 %) and a respective nitrate rise in the effl uent.Nevertheless, more than 90 % of COD reduction was achieved.
Aerobic fi lters are applied to a lesser extent in the treatment of high-strength dairy effl uents rich in FOG.High fat and heavy biofi lm blockage are possible, which results in biomass loss, fi lter fouling and corresponding reduction in productivity (1).
The sequencing batch reactor (SBR) is preferred in dairy wastewater treatment because of its various loading capabilities and effl uent fl exibility.A traditional technology with free sludge fl ocs is mostly applied.The purifi cation of milk effl uents is given by Britz et al. (1).COD was reduced by 91-97, TS by 63, volatile solids (VS) by 66, TKN by 75, and TN by 38 %.However, mechanical treatment had to be applied fi rst.Another study shows the aerobic SBR as an excellent example of the combination of activated sludge granulation with dairy effl uent treatment (71).Granulation stability is limited by nutrient concentration in the wastewater, while effl uent quality depends on the need for preliminary sludge sett ling, usually 0.25-0.5 HRT.Up to 90 % of total COD, 80 % of TN and 67 % of TP were reached in an 8-hour cycle and 50 % volume exchange ratio.The results were obtained aft er fully activated sludge granulation and consecutive biomass sedimentation.The soluble effl uent COD was reduced to 125 mg/L.Industrial effl uents are more diffi cult to treat than synthetic ones.The lower maximum OLRs also reduced the SBR granular sludge effi ciency (17).In a bench-scale SBR, raw industrial dairy wastewater was treated with Lactobacillus casei TISTR 1500 (62).Microaerobic conditions maintained in the SBR allow for biomass accumulation in large amounts, leading to 85 % lactose reduction via rapid fermentation and subsequent protein coagulation by 90 %.As a consequence, 70 % of COD degradation can be achieved.Around 2.67 times higher OLR was achieved in two laboratory aerobic SBRs treated with a mixed landfi ll and dairy effl uent than in traditional SBR processes (71).The best BOD 5 removal mode was reached at OLR, expressed as BOD 5 , of 0.8 kg/(m 3 •day) per a 10--day HRT.The application of fl exible fi bre as an activated sludge carrier increases the laboratory SBR reliability and it is possible to treat dairy effl uents at very high OLRs.At OLR, expressed as COD, of 0.4 kg/(m 3 •day), COD was degraded by more than 89 % and up to 97 % at OLR, expressed as COD, of 2.74 kg/(m 3 •day) (72).Membrane technologies are successfully applied in the treatment of low-load dairy effl uents in an SBR.A high BOD removal (over 97 %) and TSS-free wastewater are obtained.Due to low infl uent loading, TN removal reaches 96 % by means of assimilation only.TP elimination reaches only 80 % after system optimisation due to the limited excess sludge disposal (73).
Moving bed biofi lm reactor (MBBR) shows very high performance when applied to dairy wastewaters: OLR increases dozens of times compared to conventional activated sludge systems.A milk processing effl uent was treated in a MBBR with biomass developed on FLOCOR--RMP ® particles (Henderson Plastics Ltd, Norfolk, UK) (74).At OLR, expressed as COD, of 5 kg/(m 3 •day), more than 80 % of total COD degradation was achieved in almost half-order kinetics with partial substrate penetration.TN was decreased by 13.3-96.2%.The small reactor volume and the high OLR encompass process applications including plant renovation and the introduction of new, limited-space treatment facilities (74).A novel MBBR with free-fl oating plastic elements (with a density slightly less than 1.0 kg/m 3 ) may give 85 and 60 % COD reduction at OLRs of 12 and 21.6 kg/(m 3 •day), respectively.On the basis of test results, we can say that the MBBR should be very suitable for the treatment of dairy industry effl uents (75).
Good results can be reached in a membrane bioreactor during the treatment of an ice-cream factory effl uent with 13.3 kg/m 3 of COD, 6.5 kg/m 3 of BOD 5 at a temperature of 25 °C.Both indicators are reduced by over 95 %, while TKN is decreased by more than 96 and TP by 80 %.Under aerobic conditions, the indigenous microfl ora composed of lactic acid bacteria may reach over 10 9 CFU/ mL, which will downgrade CIP-induced alkaline pH variations (76).
Various alternatives for aerobic treatment of dairy effl uents are also used.Pure oxygen is another possibility in the biodegradation of milk wastewater.Oxygen can be applied directly in the homogenisation tank during a traditional physicochemical treatment and stable operation is achieved under a broad initial COD and TSS range.This modifi cation improves effl uent quality and reduces process costs.Such oxygen injection systems can replace the expensive anaerobic treatment and are naturally safer (77).Cheese whey can also be successfully utilised as a cheap medium for edible mushroom cultivation.Some authors report the growth of Ganoderma lucidum on protein-free cheese whey.The best soluble COD utilisation was achieved at pH=4.6 and 27.1 °C, while the maximum mycelial yield of 0.35 mg per mg of soluble COD removed was obtained at pH=4.2 and 28.5 °C (78).Although there is information on edible fungal growth, dairy wastewater utilisation has not been studied from a COD point of view (79)(80)(81)(82).
Cheese whey effl uents can be treated successfully in municipal wastewater treatment plants.Factories with onsite treatment technologies should collect sanitary wastewater independently from processing effl uents and discharge them directly into municipal wastewater treatment plants.Nevertheless, such a treatment option can lead to operational problems with secondary treatment units (1,12).Periodic sludge bulking is possible and is caused by intermitt ent high soluble COD levels in the receiving sewage plant.Anaerobic processes.Anaerobic systems are more suitable for the direct utilisation of high-strength dairy wastewater and are more cost-eff ective than aerobic processes.If properly operated, these systems do not produce unpleasant odours (1,4).The major problems of anaerobic dairy wastewater treatment include long start-up periods due to complex substrate degradation, preliminary biomass adaptation prior to protein and fat utilisation, fast drop in pH and a resultant inhibition of methane production (as a consequence of the high concentration of easily fermentable lactose and low substrate alkalinity), sludge disintegration by fats in the form of triglyceride emul-sions and subsequent biomass fl otation, presence of inhibitory compounds (long-chain fatt y acids, K + and Na + ions), inability of ammonia biodegradation and phosphorus removal, careful management, increased sensitivity to various OLRs and shock loadings, etc. Notwithstanding the litt le information on industrial-scale anaerobic plants utilising cheese whey, more than 75 % COD removal and around 10 kg/(m 3 •day) of OLRs, expressed as COD, are achieved.The degree of biodegradation depends on the HRT applied (4,12,22,(83)(84)(85).
Milk processing effl uents are predominantly treated in conventional one-phase systems: upfl ow anaerobic sludge blanket (UASB) reactor and anaerobic fi lter (AF) are most commonly applied (4).UASB reactors have been used in industrial dairy wastewater treatment for more than 20 years.They are suitable for treatment of overloaded effl uents with COD higher than 42 g/L (86).Laboratory scale UASB reactors utilising whey permeates in a continuous regime have been designed (87).Kinetic coefficients using the Monod equation are determined per HRT of 0.4-5 days and an initial wastewater COD of 10.4-0.2 g/L (87).It was shown by a comparative study of the possibility of using fl occulent sludge and the eff ect of diff erent HRTs (6-16 h) on the anaerobic UASB reactor behaviour applied to dairy wastewater treatment that nearly 80 % of protein mineralisation, soluble COD and volatile fatt y acid degradation as well as over 60 % fat removal can be reached at an HRT of at least 12 h and an OLR, expressed as COD, of less than 2.5 g/(L•day) (88).Biomass granulation was also achieved in the UASB reactor within 60-70 days.Of all the elements studied, only Ca 2+ ions had any signifi cant eff ect (89).When treating a synthetic ice-cream effl uent in the UASB reactor, TOC was reduced by 86 % at an HRT of 18.4 h, with the highest OLR, expressed as TOC, reaching 3.06 kg/(m 3 •day) (1).High FOG degradation is also possible in an UASB reactor.A couple of bench-scale UASB reactors were successfully employed during the utilisation of a synthetic milk effl uent rich in FOG (0.2, 0.6 and 1 g/L) (90).Enzymatic pre-hydrolysis contributed to 8 % more COD removal at the highest FOG concentration (90).Cheese effl uents are degraded in UASB reactors in laboratory tests and on an industrial scale.A laboratory-scale UASB reactor utilising a cheese factory effl uent eliminates around 90 % of effl uents at an OLR, expressed as COD, of 31 g/(L•day) (91).Organic loads, expressed as COD, over 45 g/(L•day) perform worse (70-80 % only).Moreover, chemicals are needed to support a constant pH.Short-shock OLR during operation increases sludge granulation, improving stability in reactor performance.The results of the laboratory tests on an industrial level have been confi rmed (1), improving them by 6 % per 10 % higher load.A full-plant UASB reactor can be applied in cheese factory wastewater treatment.With an initial COD of 33 g/L, HRT of 16 h and OLR, expressed as COD, of 49.5 kg/(m 3 •day), 86 % degradation can be reached.During the utilisation of an industrial effl uent from Edam cheese, butt er and milk production, a full-scale UASB reactor can be applied, the COD being decreased by 70 % (1).
Dairy effl uents with a low TSS can be successfully utilised in AFs in an all-scale range.The COD decreased by between 60 and 98 % at a HRT of 12-48 h and an OLR, expressed as COD, of 1.7-20 kg/(m 3 •day) (1).A large specifi c surface of the fi lter media creates a precondition for higher biomass accumulation which is less aff ected by shear stress.A fi ve-time higher load than with the non--porous fi ller under the same conditions is achieved.It has been reported that with a couple of mesophilic upfl ow AFs utilising a milk bott ling effl uent, the reactor with the porous packing performed bett er (OLR, expressed as COD, of 21 kg/(m 3 •day)) than the same reactor with non-porous packing (OLR, expressed as COD, of 4 kg/(m 3 •day)), which is infl uenced by shear stress to a greater extent (92).Different temperature regimes can be analysed during the treatment of dairy wastewater in la boratory upfl ow AFs.At 12.5, 21 and 30 °C and HRT of 4 days on average, the COD removal in each reactor amounted to 92, 85 and 78 %, respectively (93).An AF was used to treat ice-cream wastewater in a comparative study with contact process, UASB reactor and fl uidised bed bioreactor (FBB) (94).The data showed a COD removal of 67, 80 and 50 % at OLR, expressed as COD, of 6, 1 and 2 kg/(m 3 •day) and 60 % of total COD removal, at OLR, expressed as COD, of 2-4 kg/ (m 3 •day).All reactors had a poor biomass retention resulting from FOG loading.An upfl ow AF performed bett er, which allowed its full-scale installation in the manufacturing process (94).An upfl ow AF has been claimed to be unsuitable for the anaerobic digestion of very dilute dairy wastewaters (95).In fact, continuous stirred-tank (CSTR), UASB and baffl ed reactors also cause problems although experimental data show that the baffl ed reactor performs bett er with an OLR, expressed as VS, of 0.117-1.303g/ (L•day) and HRTs between 18.8 and 2 days.
Although a CSTR is a good option for scientifi c research of complete-mix systems (96), it is diffi cult to use it on an industrial scale because of HRT restrictions.Such reactors were studied with a cheese effl uent consisting of wash water/whey ratio of 4:1 with 17 g/L of COD.However, problems with sludge loss arise if the HRT drops to below 9 days (1).
Milk processing effl uents can be treated in hybrid systems too (4).An anaerobic contact digester may reach a COD degradation of over 80-95 % under mesophilic conditions.The main disadvantage is the diffi cult sludge sett lement.However, the technology is applied worldwide in dairy plants although it is quite old (1).A laboratory-scale experiment analysed the kinetic performance of anaerobic synthetic ice-cream effl uent at 37 °C applying the Monod and Contois equations at an HRT range between 2.99 and 7.45 days.A bett er explanation of the kinetic coeffi cients can be achieved in the fi nal pilot-scale plant since it allows variations in the initial substrate concentration (97).
Anaerobic packed-bed bioreactor (PBB) can be successfully applied for dairy wastewater treatment of various organic loads.A downfl ow PBB was used for treating deproteinised cheese whey with 59 g/L of COD (1).At OLR, expressed as COD, of 12.5 kg/(m 3 •day), the system decreased the COD to 90-95 % at HRT of 2-2.5 days.The infl uent pH was around 2.9, while the pH in the reactor was almost neutral.Good results were obtained in a pilot--scale plant with an up-fl ow anaerobic PBB (98).The initial cheese whey COD was 59.4 g/L.A 16-hour HRT was enough to reach 99.4 % of lactose conversion.Whey wastewater was degraded to 89 % in an anaerobic MBBR at (35±2) °C per 1-day HRT and an OLR, expressed as COD, of 11.6 kg/(m 3 •day) (99).The cheese whey was decomposed in a laboratory PBB with a polyethylene carrier.The highest COD reduction was achieved at a 3.5-day HRT with OLR, expressed as COD, of 3.8 kg/(m 3 •day) and biogas production of 0.42 m 3 per kg of COD per day (1).The mesophilic anaerobic fl uidized-bed bioreactor system degraded 5.2 g/L of COD in the ice-cream wastewater to 94.4 % at 35 °C, OLR, expressed as COD, of 15.6 kg/ (m 3 •day) and HRT of 8 h.Under shock loading, the return to steady-state conditions was possible within 6-16 h (100).The fl uidized-bed bioreactor was used to treat a low-load milk effl uent with 0.2-0.5 g/L of COD.At an 8-hour HRT, 80 % of COD was removed (1).
Membrane applications in anaerobic systems are good options for improved effl uent fi ltration combined with a higher concentration and an eff ective diff erentiation between HRT and solids retention time.A completely mixed anaerobic microfi ltration membrane reactor system was used on cheese whey high in COD (63 g/L) (1).More than 99 % of organic matt er was utilised when HRT was 7.5 days, which allowed authors to upgrade the studies from the pilot plant to a full-scale demonstration.The application of the ultrafi ltration system made it possible to achieve a higher biomass retention for more effi cient wastewater treatment.
Diff erent temperature conditions have been tested in order to reach a higher COD anaerobic removal.The psychrophilic anaerobic operation in some laboratory hybrid reactors, utilising whey effl uents with low (COD of 1 kg/ m 3 ) and high (COD of 10 kg/m 3 ) load, showed a bett er COD performance when the OLR reached 70-80 % in the fi rst reactor (at OLRs, expressed as COD, of 0.5-1.3kg/ (m 3 •day), in a 20-12 °C range) and more than 90 % in the second (at OLRs, expressed as COD, up to 13.3 kg/ (m 3 •day), in a 20-14 °C range) (101).If the high-load reactor was operated at 12 °C, COD removal decreased to 50-60 % and biogranule decomposition started.These side eff ects could be eliminated via an OLR reduction down to 6.6 kg/(m 3 •day).However, dairy wastewater has higher average temperature, which makes it possible to apply high-load wastewater treatment technologies (6,18).Another study showed that mesophilic conditions ((36±1) °C) generate more H 2 compared to thermophilic ones ((55±1) °C) during the treatment of cheese whey wastewater, with 9.2 and 8.1 mmol of H 2 per g of COD, respectively.The specifi c H 2 production was 4.6 times higher at 36 than at 55 °C (102).
Separated-phase systems are preferred from technological point of view.They have the highest organic loading and shortest HRT compared to other anaerobic digesters.The consecutive acidogenic-methanogenic phase division of anaerobic digestion is suitable for the treatment of dairy wastewater with an unbalanced composition (high C:N ratios which acidify very quickly).In such separated-phase systems, the acidogenic reactor has a major role as it supplies short-chain volatile fatt y acids which can be easily fermented to CH 4 in the methanogenic reactor.The easily utilisable lactose requires a shorter HRT and a smaller volume of the acidogenic reactor than the methanogenic digester (1,4,103).Such a system was used to treat a dairy effl uent with 50 kg/m 3 of COD and pH=4.5.The COD was decreased by 72 % at 35 °C and the following operating conditions: OLR, expressed as COD, of 50 and 9 kg/(m 3 •day), when HRT was only 1 and 3.3 days in the acidogenic and the methanogenic reactors, respectively (1).The CSTR was the preferable model for the acidogenic phase.In a 9-month operation study, a two--phase anaerobic reactor comprising an acidogenic-phase CSTR and a methanogenic-phase upfl ow AF was used to treat dairy waste streams (104).The effl uent COD was reduced by 90 % and the BOD 5 by 95 %, while an OLR, expressed as COD, of 5 kg/(m 3 •day) and a 2-day HRT were obtained.The H 2 and subsequent CH 4 production from fresh cheese whey were achieved in a CSTR, at 35 °C and HRT of 1 day.The mixed liquor was consequently fermented to CH 4 in a baffl ed bioreactor, operated at HRTs of 20, 10 and 4.4 days.At the lowest HRT, the COD reduction reached 94 % ( 105).An acidogenic CSTR and a fi nal methanogenic upfl ow AF were used to utilise cheese whey.The results showed that a maximum acidogenesis of up to 50 %, with the same OLR (expressed as COD) range (0.5-2 g per mixed liquor suspended solids per day) could be achieved at an HRT of 24 h.The effl uent was fed subsequently to the upfl ow AF where the initial soluble COD was decreased by 90 % during HRT of 4 days (106).A two-stage hybrid UASB reactor, fi lled respectively with polyurethane foam and polyvinyl chloride rings in each phase, was supposed to exceed other anaerobic methods in the treatment of dairy effl uents.The combined COD removal in the reactor in a stable equilibrium (10.7 to 19.2 kg/(m 3 •day)) changed from 97 to 99 % (39).Anaerobic rotating biological contact reactors are also discussed in the literature for anaerobic separate phase treatment (1).Carrier incorporation into anaerobic reactors for biomass support greatly increases their specifi c activity.Depending on the operating temperature, dairy wastewater can be treated in a two-phase separation.The basic confi guration presupposes that thermophilic acidogenesis is followed by mesophilic methanogenesis.The information on these processes in the literature is scarce (107)(108)(109).An experiment compared two couples of anaerobic SBRs working at the following temperatures: the fi rst couple (thermophilic-mesophilic system) at 55-35 °C and the second (mesophilic-mesophilic system) at 35-35 °C.At an OLR, expressed as VS, varying between 2-4 g/(L•day), the thermophilic-mesophilic system performs bett er (VS removal rate of 43.8-44.1 % when HRT is 3 days and 37.1-38.9% when HRT is 6 days) than the mesophilic-mesophilic system (VS removal rate of 29.3-30.2% when HRT is 3 days and 26.1-29.1 % when HRT is 6 days).The overall improved performance showed that the thermophilic-mesophilic system with respect to total coliform reduction, TSS removal and biogas production, is preferable to the mesophilic-mesophilic SBR couple.Despite that, higher energy consumption during the thermophilic phase should be taken into account from an economical point of view (84).During a set of experiments, a high-temperature-based technology including acetic and butyric acid fermentation followed by CH 4 production achieved 116 % COD reduction and 43 % CH 4 biosynthesis, thus performing bett er than single--phased processes (110).
Combined (anaerobic-aerobic) processes.Since an anaerobic technology reduces mostly C-containing contaminants and has a weaker eff ect on nutrient removal, it needs to be considered as only a preliminary step which must be polished.This can be achieved by incorporating a local aerobic step or, occasionally, by directly discharging anaerobic effl uent into the municipal wastewater treatment plants (4).
A mixed dairy wastewater was purifi ed on a full--scale level in consecutive UASB reactor and aerobic denitrifi cation steps.When 95 % COD removal was achieved, the produced CH 4 was suffi cient to cover the plant energy requirements (1).SBR great fl exibility makes it an adequate post-aerobic step in combined dairy wastewater treatment.A new downfl ow-upfl ow hybrid reactor containing downfl ow pre-acidifi cation and upfl ow methanation chambers was designed to treat high-load cheese wastewater at an average OLR, expressed as COD, of 10 g/(L•day).COD (98 %) was converted into biogas, while the discharged soluble COD reached 1 g/L.The process was maintained at stable pH values without chemical addition.Aft er treatment in the SBR, more than 90 % of COD, nitrogen from NH 3 and TP were removed (32).Wastewaters from raw milk quality laboratories, containing milk preservatives (sodium azide or chloramphenicol), were utilised in an industrial-scale plant with an AF and SBR.Infl uent FOG were completely treated in the anaerobic step without biomass washout for more than 2 years of operation, the COD decrease being more than 90 % at an OLR, expressed as COD, of 5-6 kg/(m 3 •day).However, alkali had to be added to reduce the critical pH drop.The outgoing stream from the anaerobic process was polished in SBR until the fi nal COD dropped to 200 mg/L and the TN to less than 10 mg/L (52).
The consecutive anaerobic-aerobic technology was used to purify reconstituted whey wastewater in a single reactor at low oxygen concentration and 20 °C.Maximum COD removal of (98±2) % was reached at total cycle time of 4 days and OLR, expressed as COD, of 0.78 g/(L•day).In accordance with specifi c biomass activity, trophic differentiation can be seen in the system: methanogens predominantly live at the bott om of the bulk liquid, while acidogens inhabit suspended fl ocs.When the soluble O 2 rose to 0.5 mg/L during the aerobic phase, the COD was reduced to (88±3) % in a 2-day total cycle time at 1.55 kg/ (m 3 •day) (111).

Conclusions
The discontinuous manufacturing process and high production heterogeneity in milk processing make it hard to outline the general dairy wastewater characteristics.Nevertheless, it can be concluded that dairy factories are large water consumers and therefore produce unstable waste streams with increased temperatures, variable pH values, high COD, BOD, FOG, N and P concentrations in combination with inhibiting cleaning agents and strong fl uctuations in all factors.However, there is litt le information on the composition of wastewater streams from certain dairy industry branches, such as the production of yoghurt and whey products, which require more att ention in future research.
Conventional aerobic activated sludge systems and percolating fi lters are not appropriate for dairy wastewater treatment.The high soluble COD values in wastewater account for the vast fi lamentous growth, which obstructs proper treatment and plant management.The application of immobilised biofi lm technologies off ers the opportunity to treat concentrated wastewater.MBBR are promising systems.However, many studies should be performed on other dairy wastewater streams, such as high FOG effl uents, acid whey, etc.
High organic contamination levels create conditions for the preference of anaerobic digestion over aerobic processes in dairy wastewater utilisation although an aerobic treatment rarely produces clear streams.This nec essitates the development of novel, more eff ective fer men tation technologies to deal with high-strength dairy effl uents.Insuffi cient information on temperature-phased anaerobic biodegradation paves the way for new research on dairy wastewater management.A major problem in the anaerobic fermentation of dairy wastewater is ammonia, known for its toxicity if generated in high concentrations.
Research can contribute a lot to the anaerobic ammonium oxidation application in the treatment of anaerobic effl uents from dairy manufacturing for an improved nitrogen removal.
The consecutive combination of fermentative and oxygen processes may be a solution for appropriate milk processing wastewater treatment.However, innovative and more compact equipment should be designed to meet the challenges associated with wastewater treatment limitations and water-quality requirements.Moreover, the replacement of outdated equipment with new machines needs to be supported by more, real-case studies, which will help us understand bett er dairy wastewater treatment.

Table 1 .
Composition of diff erent milk products