Research Article
BibTex RIS Cite

Formulation and physicochemical characterization of neem oil nanoemulsions for control of Sitophilus oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae)

Year 2018, Volume: 42 Issue: 2, 127 - 139, 31.05.2018
https://doi.org/10.16970/entoted.398541

Abstract

This report describes the development of environmentally-benign nanoemulsion formulations for control of adult Sitophilus oryzae(L., 1763) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae). The formulations were emulsions of neem [Azadirachta indicaA. Juss (Sapindales: Meliaceae)] oil, nonionic surfactant (alkylpolyglucoside or polysorbate 80), and water using low-energy emulsification method. Four formulations were chosen and physicochemical characterization showed a particle size range of 208-507 nm in diameter. Bioassays were conducted using food and filter paper impregnation methods. The formulation comprised of polysorbate surfactant with 2.0 mL/kg azadirachtin caused a 100% mortality of S. oryzaeadults after only 24 h of exposure, with the food impregnation method. The toxicological studies carried out at Putra University of Malaysia toxicology laboratory between November 2014 and December 2015 indicated that S. oryzaeadults were more susceptible than T. castaneumadults to nanoemulsion formulations including 2.0 mL/kg azadirachtin according to median lethal times.

References

  • Anjali, C. H., Y. Sharma, A. Mukherjee & N. Chandrasekaran, 2012. Neem oil (Azadirachta indica) nanoemulsion a potent larvicidal agent against Culex quinquefasciatus. Pest Management Science, 68: 158-163.
  • Asib, N., D. Omar, R. M. Awang, N. Ashikin & P. Abdullah, 2015. Preparation, characterization and toxicity of nano-emulsion formulations of rotenone extract of Derris Elliptica. Journal of Chemical Biological and Physical Sciences, 5: 3989-3997.
  • Bell, C. H. & S. M. Wilson, 1995. Phosphine tolerance and resistance in Trogoderma granarium Everts (Coleoptera: Dermestidae). Journal of Stored Products Research, 31: 199-205.
  • Chen, G. & D. Tao, 2005. An experimental study of stability of oil-water emulsion. Journal of Fuel Processing Technology, 86: 499-508.
  • Choupanian, M., D. Omar, M. Basri & N. Asib, 2017. Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults. Journal of Pesticide Science, 42: 158-165.
  • Costa, J. T., M. R. Forim, E. S. Costa, J. R. D. Souza, J. M. Mondego & A. L. B. Junior, 2014. Effects of different formulations of neem oil-based products on control Zabrotes subfasciatus (Boheman, 1833) (Coleoptera: Bruchidae) on beans. Journal of Stored Products Research, 56: 1-5.
  • Domínguez Umpiérrez, J. E. & L. Marrero Artabe, 2010. Catálogo de la entomofauna asociada a almacenes de alimentos en la provincia de Matanzas. Fitosanidad, 14: 75-82.
  • Finney, D., 1971. Probit Analysis (3rd Edition). Cambridge University Press, Cambridge, UK, 333 pp.
  • Flanangan, J., K. Kortegaard, D. N. Pinder, T. Rades & H. Singh, 2006. Solubilisation of soybean oil in microemulsions using various surfactants. Food Hydrocolloids, 20: 253-260.
  • Gahukar, R. T., 2014. Factors affecting content and bioefficacy of neem (Azadirachta indica A, Juss.) phytochemicals used in agricultural pest control: A review. Journal of Crop Protection, 62: 93-99.
  • Gonzalez, J. O. W., M. M. Gutierrez & A. A. Ferrero, 2013. Essential oils of nanoformulations for stored-product pest control-characterization and biological properties. Chemosphere, 100: 130-138.
  • Hameed, A., S. Freed, A. Hussain, M. Iqbal, M. Hussain, M. Naeem & A. L. Tipu, 2012. Toxicological effects of neem (Azadirachta indica), Kaniar (Nerium oleander) and spinosad (Tracer 240 SC) on the red flour beetle Tribolium castaneum (Herbst.). African Journal of Agricultural Research, 7: 555-560.
  • Howe, R. W., 1965. Losses caused by insects and mites in stored foods and food stuffs. Brazilian Journal of Nutrition, 35: 285-302.
  • Islam, M. S., M. M. Hasan, C. Lei, T. Mucha-Pelzer, I. Mewis & C. Ulrichs, 2010. Direct and admixture toxicity of diatomaceous earth and monoterpenoids against the storage pests Callosobruchus maculatus (F.) and Sitophilus oryzae (L.). Journal of Pest Science, 83: 105-112.
  • Kljajic, P. & I. Peric, 2006. Susceptibility to contact insecticides of granary weevil Sitophilus granarius (L.) (Coleoptera: Curculionidae) originating from different locations in the former Yugoslavia. Journal of Stored Products Research, 42: 149-161.
  • Lim, C. J., M. Basri, D. Omar, M. B. Abdul Rahman, A. B. Salleh & R. N. Z. R. A. Rahman, 2013. Green nanoemulsion-laden glyphosate isopropylamine formulation in suppressing creeping foxglove (A. gangetica), slender button weed (D. ocimifolia) and buffalo grass (P. conjugatum). Pest Management Sciece, 69: 104-111.
  • Lin, J. J. & S. F. Lin, 2003. Phase inversion of self-aggregation Mannich amines with poly (oxyethylene) segments. Journal of Colloid and Interface Science, 258: 310-321.
  • Lu, J. H. & Y. Q. He, 2010. Fumigant toxicity of Ailanthus altissima (Swingle), Atractylodes lancea (Thunb.) DC. and Elsholtzia atauntonii Benth extracts on three major stored-grain insects. Industrial Crops and Products, 32: 681-683.
  • Mazzonetto, F. & J. D. Vendramim, 2003. Effect of powders from vegetal species on Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae) in stored bean. Neotropical Entomology, 32: 145-149.
  • Medeiros, D. C., R. C. Andrade-Neto, L. K. Figueira, D. K. Nery, P. B. Maracaja & G. H. S. Nunes, 2007. Dry and green leaves powder of neem in the control of the woodworm in Cowpea seed. Revista Cientifica Eletrȏnica de Engenharia Florestal, 10: 1.
  • Mishra, P., J. Jerobin, J. Thomas, A. Mukherjee & N. Chandrasekaran, 2014. Study on antimicrobial potential of neem oil nanoemulsion against Pseudomonas aeruginosa infection in Labeo rohita. Biotechnology and Applied Biochemistry, 61: 611-619.
  • Morales, D., J. M. Gutierrez, M. J. Garci-Celma & Y. C. Solans, 2003. A study of the relation between bicontinuous microemulsions and oil/water nano-emulsion formation. Langmuir, 17: 7196-7200.
  • Negahban, M., S. Moharramipour & F. Sefidkon, 2007. Fumigant toxicity of essential oil from Artemisia sieberi Besser against three stored-product insects. Journal of Stored Products Research, 43: 123-128.
  • Ogendo, J. O., M. Kostyukovsky, U. Ravid, J. C. Matasyoh, A. L. Deng, E. O. Omolo, S. T. Kariuki & E. Shaaya, 2008. Bioactivity of Ocimum gratissimum (L.) oil and two of its constituents against five insect pests attacking stored food products. Journal of Stored Products Research, 44: 328-334.
  • Osborne, J. W., 2010. Improving your data transformation: applying the Box-Cox transformation. Practical Assessment, Research & Evaluation, 15: 1-9.
  • Reiss, H., 1975. Entropy induced dispersion of bulk liquid. Journal of Colloid and Interface Science, 53: 61-70.
  • Sahaf, B. Z., S. Moharramipour & M. H. Meshkatalsadat, 2007. Chemical constituents and fumigant toxicity of essential oil from Carum copticum against two stored product beetles. Insect Science, 14: 213-218.
  • Sahaf, B. Z., S. Moharramipour & M. H. Meshkatalsadat, 2008. Fumigant toxicity of essential oil from Vitex pseudo-negundo against Tribolium castaneum (Herbst) and Sitophilus oryzae (L.). Journal of Asia-Pacific Entomology, 11: 175-179.
  • Shafiq, S., S. Faiyaz, T. Sushma, J. A. Farhan, A. K. Khar & M. Ali, 2007. Design and development of oral oil in water ramipril nanoemulsion formulation: in vitro and in vivo assessment. Journal Biomedical Nanotechnology, 3: 28-44.
  • Solans, C., J. Esquena, A. Forgiarini, N. Uson, D. Morales, P. Izquierdo, N. Azemar & M. García-Celma, 2003. "Nano-emulsions: Formation, Properties and Applications, 525-554". In: Adsorption and Aggregation of Surfactants in Solution, Vol. 109 (Eds. K. L. Mittal & D. O. Shah). Marcel Dekker, New York, USA. 724 pp.
  • Solans, C., P. J. Izquierdo, J. Nolla, N. Azemar, M. J. Garcia-Celma, 2005. Nano-emulsion. Current Opinion in Colloid & Interface Science, 10: 102-110.
  • Taghizadeh-Saroukolai, A., S. Moharramipour & M. H. Meshkatalsadat, 2010. Insecticidal properties of Thymus persicus essential oil against Tribolium castaneum and Sitophilus oryzae. Journal of Pest Science, 83: 3-8.
  • Talukder, F. & P. Howse, 1994. Laboratory evaluation of toxic and repellent properties of the pithraj tree, Aphanamixis polystachya Wall & Parker, against Sitophilus oryzae (L.). International Journal of Pest Management, 40: 274-279.
  • Taylor, P., 2003. Ostwald ripening in emulsions: Estimation of solution thermodynamics of the disperses phase. Advanced Colloid Interface, 106: 261-285.
  • Wagan, T. A., D. Hu, Y. He, M. Nawaz, T. Nazir, J. I. Mabubu & H. Hua, 2016. Repellency of three plant essential oils against red flour beetle Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebriondae). Turkish Journal of Entomology, 40: 347-354.
  • Weaver, D. K., C. D. Wells, F. V. Dunkell, W. Bertsch, S. E. Sing & S. Shiharan, 1994. Insecticidal activity of floral, foliar, and root extracts of Tagetes minuta (Asterales: Asteraceae) against adult Mexican bean weevil (Coleoptera: Bruchidae). Journal of Economic Entomology, 87: 1718-1725.

Sitophilus oryzae (L., 1763) (Coleoptera: Curculionidae) ve Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae)'un kontrolünde neem yağının nano emülsiyonlarının ve fizikokimyasal tanımlaması ve formülasyonu

Year 2018, Volume: 42 Issue: 2, 127 - 139, 31.05.2018
https://doi.org/10.16970/entoted.398541

Abstract

Bu çalışmada, Sitophilus oryzae(L., 1763) (Coleoptera: Curculionidae) ve Tribolium castaneum(Herbst, 1797) (Coleoptera: Tenebrionidae) erginlerin mücadelesinde kullanmak için çevre dostu nano emülsiyon formülasyonlarının geliştirilmesini amaçlanmıştır. Emülsiyon formülasyonu, düşük enerjili emülsiyonlaşma yöntemi kullanılarak neem yağından [Azadirachta indicaA. Juss (Sapindales: Meliacear)], iyonik olmayan yüzey aktif maddeden (alkilpoliglukosid veya polisorbat 80) ve sudan oluşturulmuştur. Dört formülasyon seçilmiş ve fizikokimyasal çapı 208-507 nm olan partikül boyutu aralığı ile karakterize edilmiştir. Biyoassay çalışmaları, besine ve filtre kağıdına emdirme yöntemleri kullanılarak yürütülmüştür. 2.0 mL/kg azadirachtin konsantrasyonlu polisorbat yüzey aktif maddeden oluşan formülasyon besine emdirme yöntemi ile sadece 24 saat maruz bırakıldığında S. oryzaeerginlerinin %100 ölümü gerçekleşmiştir. Kasım 2014 ile Aralık 2015 tarihleri arasında Putra Malezya Üniversitesi toksikoloji laboratuvarında yürütülen toksikolojik çalışmalar ortalama ölüm zaman değerlerine göre S. oryzaeerginleri 2.0 mL/kg neem yağı içeren nano-emülsiyon formülasyona karşı en hassas tür olduğunu göstermiştir.

References

  • Anjali, C. H., Y. Sharma, A. Mukherjee & N. Chandrasekaran, 2012. Neem oil (Azadirachta indica) nanoemulsion a potent larvicidal agent against Culex quinquefasciatus. Pest Management Science, 68: 158-163.
  • Asib, N., D. Omar, R. M. Awang, N. Ashikin & P. Abdullah, 2015. Preparation, characterization and toxicity of nano-emulsion formulations of rotenone extract of Derris Elliptica. Journal of Chemical Biological and Physical Sciences, 5: 3989-3997.
  • Bell, C. H. & S. M. Wilson, 1995. Phosphine tolerance and resistance in Trogoderma granarium Everts (Coleoptera: Dermestidae). Journal of Stored Products Research, 31: 199-205.
  • Chen, G. & D. Tao, 2005. An experimental study of stability of oil-water emulsion. Journal of Fuel Processing Technology, 86: 499-508.
  • Choupanian, M., D. Omar, M. Basri & N. Asib, 2017. Preparation and characterization of neem oil nanoemulsion formulations against Sitophilus oryzae and Tribolium castaneum adults. Journal of Pesticide Science, 42: 158-165.
  • Costa, J. T., M. R. Forim, E. S. Costa, J. R. D. Souza, J. M. Mondego & A. L. B. Junior, 2014. Effects of different formulations of neem oil-based products on control Zabrotes subfasciatus (Boheman, 1833) (Coleoptera: Bruchidae) on beans. Journal of Stored Products Research, 56: 1-5.
  • Domínguez Umpiérrez, J. E. & L. Marrero Artabe, 2010. Catálogo de la entomofauna asociada a almacenes de alimentos en la provincia de Matanzas. Fitosanidad, 14: 75-82.
  • Finney, D., 1971. Probit Analysis (3rd Edition). Cambridge University Press, Cambridge, UK, 333 pp.
  • Flanangan, J., K. Kortegaard, D. N. Pinder, T. Rades & H. Singh, 2006. Solubilisation of soybean oil in microemulsions using various surfactants. Food Hydrocolloids, 20: 253-260.
  • Gahukar, R. T., 2014. Factors affecting content and bioefficacy of neem (Azadirachta indica A, Juss.) phytochemicals used in agricultural pest control: A review. Journal of Crop Protection, 62: 93-99.
  • Gonzalez, J. O. W., M. M. Gutierrez & A. A. Ferrero, 2013. Essential oils of nanoformulations for stored-product pest control-characterization and biological properties. Chemosphere, 100: 130-138.
  • Hameed, A., S. Freed, A. Hussain, M. Iqbal, M. Hussain, M. Naeem & A. L. Tipu, 2012. Toxicological effects of neem (Azadirachta indica), Kaniar (Nerium oleander) and spinosad (Tracer 240 SC) on the red flour beetle Tribolium castaneum (Herbst.). African Journal of Agricultural Research, 7: 555-560.
  • Howe, R. W., 1965. Losses caused by insects and mites in stored foods and food stuffs. Brazilian Journal of Nutrition, 35: 285-302.
  • Islam, M. S., M. M. Hasan, C. Lei, T. Mucha-Pelzer, I. Mewis & C. Ulrichs, 2010. Direct and admixture toxicity of diatomaceous earth and monoterpenoids against the storage pests Callosobruchus maculatus (F.) and Sitophilus oryzae (L.). Journal of Pest Science, 83: 105-112.
  • Kljajic, P. & I. Peric, 2006. Susceptibility to contact insecticides of granary weevil Sitophilus granarius (L.) (Coleoptera: Curculionidae) originating from different locations in the former Yugoslavia. Journal of Stored Products Research, 42: 149-161.
  • Lim, C. J., M. Basri, D. Omar, M. B. Abdul Rahman, A. B. Salleh & R. N. Z. R. A. Rahman, 2013. Green nanoemulsion-laden glyphosate isopropylamine formulation in suppressing creeping foxglove (A. gangetica), slender button weed (D. ocimifolia) and buffalo grass (P. conjugatum). Pest Management Sciece, 69: 104-111.
  • Lin, J. J. & S. F. Lin, 2003. Phase inversion of self-aggregation Mannich amines with poly (oxyethylene) segments. Journal of Colloid and Interface Science, 258: 310-321.
  • Lu, J. H. & Y. Q. He, 2010. Fumigant toxicity of Ailanthus altissima (Swingle), Atractylodes lancea (Thunb.) DC. and Elsholtzia atauntonii Benth extracts on three major stored-grain insects. Industrial Crops and Products, 32: 681-683.
  • Mazzonetto, F. & J. D. Vendramim, 2003. Effect of powders from vegetal species on Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae) in stored bean. Neotropical Entomology, 32: 145-149.
  • Medeiros, D. C., R. C. Andrade-Neto, L. K. Figueira, D. K. Nery, P. B. Maracaja & G. H. S. Nunes, 2007. Dry and green leaves powder of neem in the control of the woodworm in Cowpea seed. Revista Cientifica Eletrȏnica de Engenharia Florestal, 10: 1.
  • Mishra, P., J. Jerobin, J. Thomas, A. Mukherjee & N. Chandrasekaran, 2014. Study on antimicrobial potential of neem oil nanoemulsion against Pseudomonas aeruginosa infection in Labeo rohita. Biotechnology and Applied Biochemistry, 61: 611-619.
  • Morales, D., J. M. Gutierrez, M. J. Garci-Celma & Y. C. Solans, 2003. A study of the relation between bicontinuous microemulsions and oil/water nano-emulsion formation. Langmuir, 17: 7196-7200.
  • Negahban, M., S. Moharramipour & F. Sefidkon, 2007. Fumigant toxicity of essential oil from Artemisia sieberi Besser against three stored-product insects. Journal of Stored Products Research, 43: 123-128.
  • Ogendo, J. O., M. Kostyukovsky, U. Ravid, J. C. Matasyoh, A. L. Deng, E. O. Omolo, S. T. Kariuki & E. Shaaya, 2008. Bioactivity of Ocimum gratissimum (L.) oil and two of its constituents against five insect pests attacking stored food products. Journal of Stored Products Research, 44: 328-334.
  • Osborne, J. W., 2010. Improving your data transformation: applying the Box-Cox transformation. Practical Assessment, Research & Evaluation, 15: 1-9.
  • Reiss, H., 1975. Entropy induced dispersion of bulk liquid. Journal of Colloid and Interface Science, 53: 61-70.
  • Sahaf, B. Z., S. Moharramipour & M. H. Meshkatalsadat, 2007. Chemical constituents and fumigant toxicity of essential oil from Carum copticum against two stored product beetles. Insect Science, 14: 213-218.
  • Sahaf, B. Z., S. Moharramipour & M. H. Meshkatalsadat, 2008. Fumigant toxicity of essential oil from Vitex pseudo-negundo against Tribolium castaneum (Herbst) and Sitophilus oryzae (L.). Journal of Asia-Pacific Entomology, 11: 175-179.
  • Shafiq, S., S. Faiyaz, T. Sushma, J. A. Farhan, A. K. Khar & M. Ali, 2007. Design and development of oral oil in water ramipril nanoemulsion formulation: in vitro and in vivo assessment. Journal Biomedical Nanotechnology, 3: 28-44.
  • Solans, C., J. Esquena, A. Forgiarini, N. Uson, D. Morales, P. Izquierdo, N. Azemar & M. García-Celma, 2003. "Nano-emulsions: Formation, Properties and Applications, 525-554". In: Adsorption and Aggregation of Surfactants in Solution, Vol. 109 (Eds. K. L. Mittal & D. O. Shah). Marcel Dekker, New York, USA. 724 pp.
  • Solans, C., P. J. Izquierdo, J. Nolla, N. Azemar, M. J. Garcia-Celma, 2005. Nano-emulsion. Current Opinion in Colloid & Interface Science, 10: 102-110.
  • Taghizadeh-Saroukolai, A., S. Moharramipour & M. H. Meshkatalsadat, 2010. Insecticidal properties of Thymus persicus essential oil against Tribolium castaneum and Sitophilus oryzae. Journal of Pest Science, 83: 3-8.
  • Talukder, F. & P. Howse, 1994. Laboratory evaluation of toxic and repellent properties of the pithraj tree, Aphanamixis polystachya Wall & Parker, against Sitophilus oryzae (L.). International Journal of Pest Management, 40: 274-279.
  • Taylor, P., 2003. Ostwald ripening in emulsions: Estimation of solution thermodynamics of the disperses phase. Advanced Colloid Interface, 106: 261-285.
  • Wagan, T. A., D. Hu, Y. He, M. Nawaz, T. Nazir, J. I. Mabubu & H. Hua, 2016. Repellency of three plant essential oils against red flour beetle Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebriondae). Turkish Journal of Entomology, 40: 347-354.
  • Weaver, D. K., C. D. Wells, F. V. Dunkell, W. Bertsch, S. E. Sing & S. Shiharan, 1994. Insecticidal activity of floral, foliar, and root extracts of Tagetes minuta (Asterales: Asteraceae) against adult Mexican bean weevil (Coleoptera: Bruchidae). Journal of Economic Entomology, 87: 1718-1725.
There are 36 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Marziyeh Choupanıan

Dzolkhifli Omar This is me

Publication Date May 31, 2018
Submission Date February 26, 2018
Acceptance Date May 4, 2018
Published in Issue Year 2018 Volume: 42 Issue: 2

Cite

APA Choupanıan, M., & Omar, D. (2018). Formulation and physicochemical characterization of neem oil nanoemulsions for control of Sitophilus oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae). Turkish Journal of Entomology, 42(2), 127-139. https://doi.org/10.16970/entoted.398541
AMA Choupanıan M, Omar D. Formulation and physicochemical characterization of neem oil nanoemulsions for control of Sitophilus oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae). TED. May 2018;42(2):127-139. doi:10.16970/entoted.398541
Chicago Choupanıan, Marziyeh, and Dzolkhifli Omar. “Formulation and Physicochemical Characterization of Neem Oil Nanoemulsions for Control of Sitophilus Oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium Castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae)”. Turkish Journal of Entomology 42, no. 2 (May 2018): 127-39. https://doi.org/10.16970/entoted.398541.
EndNote Choupanıan M, Omar D (May 1, 2018) Formulation and physicochemical characterization of neem oil nanoemulsions for control of Sitophilus oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae). Turkish Journal of Entomology 42 2 127–139.
IEEE M. Choupanıan and D. Omar, “Formulation and physicochemical characterization of neem oil nanoemulsions for control of Sitophilus oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae)”, TED, vol. 42, no. 2, pp. 127–139, 2018, doi: 10.16970/entoted.398541.
ISNAD Choupanıan, Marziyeh - Omar, Dzolkhifli. “Formulation and Physicochemical Characterization of Neem Oil Nanoemulsions for Control of Sitophilus Oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium Castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae)”. Turkish Journal of Entomology 42/2 (May 2018), 127-139. https://doi.org/10.16970/entoted.398541.
JAMA Choupanıan M, Omar D. Formulation and physicochemical characterization of neem oil nanoemulsions for control of Sitophilus oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae). TED. 2018;42:127–139.
MLA Choupanıan, Marziyeh and Dzolkhifli Omar. “Formulation and Physicochemical Characterization of Neem Oil Nanoemulsions for Control of Sitophilus Oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium Castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae)”. Turkish Journal of Entomology, vol. 42, no. 2, 2018, pp. 127-39, doi:10.16970/entoted.398541.
Vancouver Choupanıan M, Omar D. Formulation and physicochemical characterization of neem oil nanoemulsions for control of Sitophilus oryzae (L., 1763) (Coleoptera: Curculionidae) and Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae). TED. 2018;42(2):127-39.