Berriochloa gabeli n. sp. and Berriochloa huletti n. sp. are described from fossil anthoecia (husks) recovered in late Miocene (Ash Hollow Formation) sediments in central Nebraska and central and western Kansas. Comparisons with other known fossil and living grasses suggest relationships with members of the grass tribe Stipeae and previously described B. intermedia Elias, 1942 from Ash Hollow deposits in north-central Kansas. Berriochloa gabeli and B. huletti were recovered in direct association with, or in the close vicinity, of rich biotas that provide evidence of widespread, probably treeless, grasslands with adjacent moist riparian habitats along streams or around temporary pools of water during deposition. Fossil vertebrates associated with the grasses at some sites suggest that the age of B. gabeli and B. huletti is early to middle Hemphillian.