Skip to main content
SpringerLink
Log in

Fluorescence microscopic analysis of bone osseointegration of strontium-substituted hydroxyapatite implants

  • Published:
Journal of Zhejiang University SCIENCE B Aims and scope Submit manuscript

Abstract

The purpose of this study was to analyze the effect of strontium-substituted hydroxyapatite (Sr-HA) on bone osseointegration of the implants using fluorescence microscopy. We allocated 20 implants to two groups: Sr-HA group and HA group. Electrochemically deposited HA and Sr-HA coatings were applied onto the implants separately. All the implants were inserted into femur bone of rabbits. Oxytetracycline hydrochloride, alizarin-complexon, and calcein green were respectively administered 7, 28, and 46 d after the implantation. After eight weeks, femurs were retrieved and prepared for the fluorescence microscopy observation. We analyzed the bone mineral apposition rates (MARs), bone area ratios (BARs), and bone to implant contact (BIC) of the two groups. Fluorescence microscopic observation showed that all groups exhibited extensive early peri-implant bone formation. The MAR of the Sr-HA group was greater than that for pure HA from 7 to 28 d after implantation, but no significant difference was found at later stage. And the BIC showed difference at 7 and 28 d compared with pure HA. We concluded that Sr-HA coating can improve the bone osseointegration of the implant in the early stage compared with the HA coating.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Boanini, E., Gazzano, M., Bigi, A., 2010. Ionic substitutions in calcium phosphates synthesized at low temperature. Acta Biomater., 6(6):1882–1894. [doi:10.1016/j.actbio.2009..12.041]

    Article  PubMed  CAS  Google Scholar 

  • Christoffersen, M.R., Christoffersen, N., Kolthoff, O., 1997. Effect of strontium ions on growth and dissolution of hydroxyapatite and on bone mineral detection. Bone, 20(1):47–54. [[doi:10.1016/S8756-3282(96)00316-X]

    Article  PubMed  CAS  Google Scholar 

  • Cooper, L.F., Zhou, Y., Takebe, J., Guo, J., Abron, A., Holmen, A., Ellingsen, J.E., 2006. Fluoride modification effects on osteoblast behavior and bone formation at TiO2 grit-blasted c.p. titanium endosseous implants. Biomaterials, 27(6):926–936. [doi:10.1016/j.biomaterials.2005.07.009]

    Article  PubMed  CAS  Google Scholar 

  • Friberg, B., Sennerby, L., Linden, B., Lekholm, U., 1999. Stability measurements of one-stage Brånemark implants during healing in mandibles. A clinical resonance frequency analysis study. Int. J. Oral Maxillofac. Surg., 28(4):266–272. [[doi:10.1016/S0901-5027(99)80156-8]

    CAS  Google Scholar 

  • Gentleman, E., Fredholm, Y.C., Jell, G., Lotfibakhshaiesh, N., O’Donnell, M.D., Hill, R.G., Stevens, M.M., 2010. The effects of strontium-substituted bioactive glasses on osteoblasts and osteoclasts in vitro. Biomaterials, 31(14): 3949–3956. [doi:10.1016/j.biomaterials.2010.01.121]

    Article  PubMed  CAS  Google Scholar 

  • Hanawa, T., Kamira, Y., Yamamoto, S., Kohgo, T., Amemyia, A., Ukai, H., Murakami, K., Asaoka, K., 1997. Early bone formation around calcium-ion-implanted titanium inserted into rat tibia. J. Biomed. Mater. Res., 36(1):131–136. [doi:10.1002/(SICI)1097-4636(199707)36:1<131::AID-JBM 16>3.0.CO;2-L]

    Article  PubMed  CAS  Google Scholar 

  • He, F., Yang, G., Wang, X., Zhao, S., 2009. Effect of electrochemically deposited nanohydroxyapatite on bone bonding of sandblasted/dual acid-etched titanium implant. Int. J. oral Maxillofac. Implants, 24(5):790–799.

    PubMed  Google Scholar 

  • Jiao, M.J., Wang, X.X., 2009. Electrolytic deposition of magnesium-substituted hydroxyapatite crystals on titanium substrate. Mater. Lett., 63(27):2286–2289. [doi:10. 1016/j.matlet.2009.07.048]

    Article  CAS  Google Scholar 

  • Jinno, T., Kirk, S.K., Morita, S., Goldberg, V.M., 2004. Effects of calcium ion implantation on osseointegration of surface blasted titanium alloy femoral implants in a canine total hip arthroplasty model. J. Arthroplasty, 19(1): 102–109. [doi:10.1016/j.arth.2003.10.001]

    Article  PubMed  Google Scholar 

  • Ni, G.X., Lin, J.H., Chiu, P.K.Y., Li, Z.Y., Lu, W.W., 2010. Effect of strontium-containing hydroxyapatite bone cement on bone remodeling following hip replacement. J. Mater. Sci. Mater. Med., 21(1):377–384. [doi:10.1007/ s10856-009-3866-2]

    Article  PubMed  CAS  Google Scholar 

  • Ni, G.X., Yao, Z.P., Huang, G.T., Liu, W.G., William, W., 2011. The effect of strontium incorporation in hydroxyapatite on osteoblasts in vitro. J. Mater. Sci. Mater. Med., 22(4):961–967. [doi:10.1007/s10856-011-4264-0]

    Article  PubMed  CAS  Google Scholar 

  • Nkenke, E., Kloss, F., Wiltfang, J., Schultze-Mosgau, S., Radespiel-Tröger, M., Loos, L., Neukam, F.W., 2002. Histomorphometric and fluorescence microscopic analysis of bone remodelling after installation of implants using an osteotome technique. Clin. Oral Implants Res., 13(6):595–602. [doi:10.1034/j.1600-0501.2002.130604.x]

    Article  PubMed  Google Scholar 

  • Park, J.W., Suh, J.Y., Chung, H.J., 2008. Effects of calcium ion incorporation on osteoblast gene expression in MC3T3-E1 cells cultured on microstructured titanium surfaces. J. Biomed. Mater. Res., 86A(1):117–126. [doi: 10.1002/jbm.a.31618]

    Article  CAS  Google Scholar 

  • Park, J.W., Kim, Y.J., Jang, J.H., 2010. Enhanced osteoblast response to hydrophilic strontium and/or phosphate ions-incorporated titanium oxide surfaces. Clin. Oral Implants Res., 21(4):398–408. [doi:10.1111/j.1600-0501. 2009.01863.x]

    Article  PubMed  Google Scholar 

  • Patel, N., Best, S.M., Bonfield, W., Gibson, I.R., Hing, K.A., Damien, E., Revell, P.A., 2002. A comparative study on the in vivo behavior of hydroxyapatite and silicon substituted hydroxyapatite granules. J. Mater. Sci. Mater. Med., 13(12):1199–1206. [doi:10.1023/A:1021114710076]

    Article  PubMed  CAS  Google Scholar 

  • Roy, M., Bandyopadhyay, A., Bose, S., 2011. Induction plasma sprayed Sr and Mg doped nano hydroxyapatite coatings on Ti for bone implant. J. Biomed. Mater. Res. B Appl. Biomater., 99B(2):258–265. [doi:10.1002/jbm.b. 31893]

    Article  CAS  Google Scholar 

  • Sato, M., Sambito, M.A., Aslani, A., Kalkhoran, N.M., Slamovich, E.B., Webster, T.J., 2006. Increased osteoblast functions on undoped and yttrium-doped nanocrystalline hydroxyapatite coatings on titanium. Biomaterials, 27(11):2358–2369. [doi:10.1016/j.biomaterials. 2005.10.041]

    Article  PubMed  CAS  Google Scholar 

  • Sila-Asna, M., Bunyaratvej, A., Maeda, S., Kitaguchi, H., Bunyaratavej, N., 2007. Osteoblast differentiation and bone formation gene expression in stroutium-inducing bone marrow mesenchymal stem cell. Kobe J. Med. Sci., 53(1–2):25–35.

    PubMed  CAS  Google Scholar 

  • Suh, J.Y., Jeung, O.C., Choi, B.J., Park, J.W., 2007. Effects of a novel calcium titanate coating on the osseointegration of blasted endosseous implants in rabbit tibiae. Clin. Oral Implants Res., 18(3):362–369. [doi:10.1111/j.1600-0501. 2006.01323.x]

    Article  PubMed  Google Scholar 

  • Sul, Y.T., 2003. The significance of the surface properties of oxidized titanium to the bone response: special emphasis on potential biochemical bonding of oxidized titanium implant. Biomaterials, 24(22):3893–3907. [doi:10.1016/ S0142-9612(03)00261-8]

    Article  PubMed  CAS  Google Scholar 

  • Sul, Y.T., Johansson, C., Byon, E., Albrektsson, T., 2005. The bone response of oxidized bioactive and non-bioactive titanium implants. Biomaterials, 26(33):6720–6730. [doi:10.1016/j.biomaterials.2005.04.058]

    Article  PubMed  CAS  Google Scholar 

  • Szmukler-Moncler, S., Piattelli, A., Favero, G.A., Dubruille, J.H., 2000. Considerations preliminary to the application of early and immediate loading protocols in dental implantology. Clin. Oral Implants Res., 11(1):12–25. [doi:10.1034/j.1600-0501.2000.011001012.x]

    Article  PubMed  CAS  Google Scholar 

  • Xue, W.C., Hosick, H.L., Bandyopadhyay, A., Bose, S., Ding, C., Luk, K.D.K., 2007. Preparation and cell-materials interactions of plasma sprayed strontium-containing hydroxyapatite coating. Surf. Coat. Technol., 201(8): 4685–4693. [doi:10.1016/j.surfcoat.2006.10.012]

    Article  CAS  Google Scholar 

  • Xue, W.C., Dahlquist, K., Banerjee, A., Bandyopadhyay, A., Bose, S., 2008. Synthesis and characterization of tricalcium phosphate with Zn and Mg based dopants. J. Mater. Sci. Mater. Med., 19:2669–2677. [doi:10.1007/s10856-008-3395-4]

    Article  PubMed  CAS  Google Scholar 

  • Yang, F., Dong, W.J., He, F.M., Wang, X.X., Zhao, S.F., Yang, G.L., 2012. Osteoblast response to porous titanium surface coated with zinc-substituted hydroxyapatite. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod., 113(3):313–318. [doi:10.1016/j.tripleo.2011.02.049]

    Google Scholar 

  • Yang, G.L., He, F.M., 2009. Effects of biomimetically and electrochemically deposited nano-hydroxyapatite coatings on osseointegration of porous titanium implants. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod., 107(6):782–789. [doi:10.1016/j.tripleo.2008.12.023]

    Article  PubMed  Google Scholar 

  • Yang, G.L., He, F.M., Zhao, S.S., Wang, X.X., Zhao, S.F., 2008. Effect of H2O2/HCl heat treatment of implants on in vivo peri-implant bone formation. Int. J. Oral Maxillofac. Implants, 23(6):1020–1028.

    PubMed  Google Scholar 

  • Yang, L., Perez-Amodio, S., Florence, Y.F., Groot, B.D., Everts, V., Blitterswijk, C.A.V., Habibovic, P., 2010. The effects of inorganic additives to calcium phosphate on in vitro behavior of osteoblasts and osteoclasts. Biomaterials, 31(11):2976–2989. [doi:10.1016/j.biomaterials.2010.01.002]

    Article  PubMed  CAS  Google Scholar 

  • Ye, W., Wang, X.X., 2007. Effect of supersaturation on the morphologies of hydroxyapatite crystal deposited on titanium surface with electrochemical technique. Key Eng. Mater., 330–332:601–604. [doi:10.4028/www.scientific.net/KEM.330-332.601]

    Article  Google Scholar 

  • Zhang, W.B., Shen, Y.H., Pan, H.B., Lin, K.L., Liu, X.G., Darvell, B.W., Lu, W.W., Chang, J., Deng, L.F., Wang, D.P., et al., 2011. Effects of strontium in modified biomaterials. Acta Biomater., 7(2):800–808. [doi:10.1016/ j.actbio.2010.08.031]

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Dentistry, Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China

    Dan-li Fu

  2. Department of Oral Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China

    Qiao-hong Jiang, Fu-ming He & Guo-li Yang

  3. Department of Prosthodontics, Stomatology Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China

    Li Liu

Authors
  1. Dan-li Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qiao-hong Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fu-ming He
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guo-li Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Li Liu.

Additional information

Project supported by the Excellent Youth Science Foundation of Zhejiang Provincial Natural Science Foundation of China (No. R2110374) and the Provincial Department Co-building Foundation of Health Bureau of Zhejiang Province, China (No. WKJ2011-2-009)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fu, Dl., Jiang, Qh., He, Fm. et al. Fluorescence microscopic analysis of bone osseointegration of strontium-substituted hydroxyapatite implants. J. Zhejiang Univ. Sci. B 13, 364–371 (2012). https://doi.org/10.1631/jzus.B1100381

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1631/jzus.B1100381

Key words

CLC number

Search

Navigation