摘要
药食两用真菌红曲霉转运真菌毒素桔霉素的途径目前尚不清楚. 本文通过CRISPR/Cas9基因编辑和过表达, 分析了紫色红曲霉中一个假定的主要协同转运蛋白超家族(MFS)基因CtnC, 证实了CtnC参与桔霉素的外排以及抑制菌丝体中桔霉素的积累, 但CtnC蛋白不是唯一通道. 同时, 本研究结果发现CtnC过表达会负调控桔霉素合成相关基因(CtnD、 CtnE、 CtnF和pksCT)的表达. 综上, 本研究发现的红曲霉中桔霉素转运体基因为真菌毒素的防控策略研究提供了新的依据.
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Acknowledgments
This work was supported by the Science and Technology Planning Project of Guizhou Province (No. 2019-2776) and the National Natural Science Foundation of China (No. 81960692).
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Jie HAN and Jiehong ZHAO designed the project and wrote the manuscript. Yanling GUI and Guangfu TANG carried out the transgenic experiment. Yanling GUI, Haiqiao MAN, and Jiao WANG conducted the chemical analysis. Guangfu TANG conducted the RT-qPCR analysis. All authors have read and approved the final manuscript, and therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Yanling GUI, Guangfu TANG, Haiqiao MAN, Jiao WANG, Jie HAN, and Jiehong ZHAO declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
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Gui, Y., Tang, G., Man, H. et al. Transportation of citrinin is regulated by the CtnC gene in the medicinal fungus Monascus purpureus. J. Zhejiang Univ. Sci. B 24, 543–548 (2023). https://doi.org/10.1631/jzus.B2300023
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DOI: https://doi.org/10.1631/jzus.B2300023