Skip to main content
Log in

Protein and hordein fraction content in barley seeds as affected by sowing date and their relations to malting quality

  • Biotechnology
  • Published:
Journal of Zhejiang University-SCIENCE B Aims and scope Submit manuscript

Abstract

The effect of sowing date on grain protein, hordein fraction content and malting quality of two-rowed spring barley was invesitigated by using ten commercial cultivars with different grain protein content and the relationships among these traits were examined. The results showed that grain protein content and B hordein content increases as the sowing date postponed and were significantly affected by sowing date, while C and D hordein contents were less influenced by sowing date. There were significant differences in grain protein and hordein fraction content among the ten cultivars. The coefficient of variation of D hordein content was much larger than that of B and C hordein contents, suggesting its greater variation caused by different sowing dates. Beta-amylase activity and diastatic power were also significantly affected by sowing date, with malt extract being less affected. Significant differences in measured malt quality were found among the ten cultivars. Grain protein was significantly correlated with B hordein and malt extract positively and negatively, respectively. There was no significant correlation between beta-amylase activity or diastatic power and grain protein content. B hordein was negatively and significantly correlated with malt extract, but no significant correlations between C hordein, D hordein and malting quality traits.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • AACC (American Association of Cereal Chemists), 2000. Approved Methods of American Association of Cereal Chemists, 10th Ed. The Association, St Paul, MN.

    Google Scholar 

  • Ahokas, H., Naskali, L., 1990. Geographic variation of α-amylase, β-amylase, β-glucanase, pullulanase and chitinase activity in germinatingHordeum spontaneum barley from Israel and Jordan.Genetica,82:73–78.

    Article  CAS  Google Scholar 

  • Arends, A.M., Fox, G.P., Henry, R.J., Marschke, R.J., Symons, M.H., 1995. Genetic and environmental variation in the diastatic power of Australian barley.J. Cereal Sci.,21:63–70.

    Article  CAS  Google Scholar 

  • Baxter, E.D., Wainwright, T., 1979. Hordein and malting quality.J. Amer. Soc. Brew. Chemist,37:8–12.

    Article  CAS  Google Scholar 

  • Birch, C.J., Long, K.E., 1990. Effect of nitrogen on the growth, yield and grain protein content of barley (Hordeum vulgare).Aust. J. Exp. Agric.,30:237–242.

    Article  CAS  Google Scholar 

  • Bishop, L.R., Day, F.E., 1993. The effect of variety on the relation between nitrogen content and extract.J. Inst. Brew.,39:545–551.

    Article  Google Scholar 

  • Brennan, C.S., Smith, D.B., Harris, N., Shewry, P.R., 1998. The production and characterization of Hor3 null lines of barley provides new information on the relationship of D hordein to malting performance.J. Cereal Sci.,28:291–299.

    Article  CAS  Google Scholar 

  • Coles, G.D., Jamieson, P.D., Haslemore, R.M., 1991. Effects of moisture stress on malting quality in Triumph barley.J. Cereal Sci.,14:161–177.

    Article  Google Scholar 

  • Delcour, J.A., Verschaeve, S.G., 1987. Malt diastatic power. Part I. A modified EBC diastatic power assay for the selective estimation of β-amylase activity. Time and temperature dependence of the release of reducing sugars.J. Inst. Brew.,93:296–301.

    Article  CAS  Google Scholar 

  • Eagles, H.A., Bedggood, A.G.J., Panozzo, F., Martin, P.J., 1995. Cultivar and environmental effects on malting quality in barley.Aust. J. Agric. Res.,46:831–844.

    Article  CAS  Google Scholar 

  • EBC (European Brewery Convention), 1975. Analytica EBC, 3rd Ed. Verlag Hans Carl, Nurnberg, Germany.

    Google Scholar 

  • Georg-Kraemer, J.E., Mundstock, E.C., Cavalli-Molina, L., 2001. Developmental expression of amylase during barley malting.J. Cereal Sci.,33:279–288.

    Article  CAS  Google Scholar 

  • Gibson, T.S., Solah, V., Glenni-Homes, M.R., Taylor, H.R., 1995. Diastatic power in malted barley: contributions of malt parameters to its development and the potential of barley grain beta-amylase to predict malt diastatic power.J. Inst. Brew.,101:277–280.

    Article  CAS  Google Scholar 

  • Grant, C.A., Gauer, L.E., Gehl, D.T., Bailey, L.D., 1991. Protein production and nitrogen utilization by barley cultivars in response to nitrogen fertilization under varying moisture conditions.Can. J. Plant Sci.,71:997–1009.

    Article  CAS  Google Scholar 

  • Howard, K.A., Gayler, K.R., Eagles, H.A., Halloran, G.M., 1996. The relationship between D hordein and malting quality in barley.J. Cereal Sci.,24:47–53.

    Article  CAS  Google Scholar 

  • Kaczmarek, K., Adamski, T., Surma, M., Jezowski, S., Lseniewska-Erstczak, M., 1999. Genotype-environment interaction of barley doubled haploids with regard to malting quality.Plant Breeding,118:243–247.

    Article  CAS  Google Scholar 

  • Macnicol, P.K., Jacobsen, J.V., Keys, M.M., Stuart, I.M., 1993. Effects of heat and water stress on malt quality and grain parameters of Schooner barley grown in cabinets.J. Cereal Sci.,18:61–68.

    Article  CAS  Google Scholar 

  • McCleary, B.V., Codd, R., 1989. Measurement of β-amylase in cereal flours and commercial enzyme preparations.J. Cereal Sci.,9:17–33.

    Article  CAS  Google Scholar 

  • Molina-Cano, J.L., Francesch, M., Perez-Vendrell, A.M., Ramo, T., Voltas, J., Brufau, J., 1997. Genetic and environmental variation in malting and feed quality of quality.J. Cereal Sci.,25:37–47.

    Article  Google Scholar 

  • Molina-Cano, J.L., Polo, J.P., Romera, E., Araus, J.L., Zarco, J., Swanston, J.S., 2001. Relationships between barley hordeins and malting quality in a mutant of cv. Triumph I. Genotype by environment interaction of hordein content.J. Cereal Sci.,34:285–294.

    Article  CAS  Google Scholar 

  • Morgan, A.G., Riggs, T.J., 1981. Effects of drought on yield and grain and malt characters in spring barley.J. Sci. Food Agric.,32:339–346.

    Article  CAS  Google Scholar 

  • Peltonen, J., Rita, H., Aikasalo, R., Home, S., 1994. Hordein and malting quality in northern barleys.Hereditas,120:231–239.

    Article  CAS  Google Scholar 

  • Rahman, S., Shewry, P.R., Miflin, B.J., 1982. Differential protein accumulation during barley grain development.J. Exper. Bot.,33:717–728.

    Article  CAS  Google Scholar 

  • Riggs, T.J., Sanada, M., Morgan, A.G., Smith, D.B., 1983. Use of acid gel electrophoresis in the characterization of “B” hordein protein in relation to mating quality and mildew resistance of barley.J. Sci. Food Agric.,34:576–586.

    Article  CAS  Google Scholar 

  • Savin, R.S., Nicolas, M.E., 1996. Effects of short periods of drought and high temperature on grain growth and starch accumulation of two malting barley cultivars.Aust. J. Plant Physiol.,23:201–210.

    Google Scholar 

  • Shewry, P.R., Faulks, A.J., Parmar, S., Miflin, B.J., 1980. Hordein polypeptide pattern in relation to malting quality and the varietal identification of malted barley grain.J. Inst. Brew.,86:138–141.

    Article  CAS  Google Scholar 

  • Shewry, P.R., Franklin, J., Parmar, S., Smith, S.J., Miflin, B.J., 1983. The effects of sulphur starvation on the amino acid and protein compositions of barley grain.J. Cereal Sci.,1:21–31.

    Article  CAS  Google Scholar 

  • Shewry, P.R., Kreis, M., Parmar, S., Lew, E.J.L., Kasarda, D.D., 1985. Identification of γ-type hordeins in barley.FEBS Lett.,190:61–64.

    Article  CAS  Google Scholar 

  • Smith, D.B., 1990. Barley seed protein and its effects on malting and brewing quality.Plant Varieties Seeds,3:63–80.

    Google Scholar 

  • Swanston, J.S., 1980. Use of electrophoresis in testing for high diastatic power in barley.J. Inst. Brew.,86:81–83.

    Article  CAS  Google Scholar 

  • Swanston, J.S., Molina-Cano, J.L., 2001. Beta-amylase activity and thermostability in two mutants derived from the malting barley cv. Triumph.J. Cereal Sci.,33:155–162.

    Article  CAS  Google Scholar 

  • Varvel, G.E., Severson, R.K., 1987. Evaluation of cultivar and nitrogen management's options for malting barley.Agron. J.,79:459–463.

    Article  Google Scholar 

  • Wang, J.M., Zhang, G.P., Chen, J.X., Shen, Q.Q., Wu, F.B., 2003. Genotypic and environmental variation in barley beta-amylase activity and its relation to protein content.Food Chem.,83:163–165.

    Article  CAS  Google Scholar 

  • Weston, D.T., Horsley, R., Schwarz, P.B., Goos, R.J., 1993. Nitrogen and planting date effects on low-protein spring barley.Agron. J.,85:1170–1174.

    Article  Google Scholar 

  • Yin, C., Zhang, G.P., Wang, J.M., Chen, J.X., 2002. Variation of beta-amylase activity in barley as affected by cultivar and environmental and its relation to protein content and grain weight.J. Cereal Sci.,36:307–312.

    Article  CAS  Google Scholar 

  • Zhang, G.P., Wang, J.M., Chen, J.X., Wu, F.B., Ding, S.R. 2005. The effect of cultivar and environment on beta-amylase activity is associated with the change of protein content in barley grains.J. Agron. Crop Sci., in Press.

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Guo-ping Zhang.

Additional information

Project supported by the National Natural Science Foundation of China (Nos. 30270779 and 30471022) and Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) (No. 20020335028), China

Rights and permissions

Reprints and permissions

About this article

Cite this article

Qi, Jc., Chen, Jx., Wang, Jm. et al. Protein and hordein fraction content in barley seeds as affected by sowing date and their relations to malting quality. J Zheijang Univ Sci B 6, 1069–1075 (2005). https://doi.org/10.1631/jzus.2005.B1069

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1631/jzus.2005.B1069

Key words

CLC number

Navigation