Abstract
Lead concentrations in roots, stems and leaves of accumulating and non-accumulating ecotypes ofSedum alfredii (Hance) were studied through a hydroponic experiment with different Pb concentrations supplied as Pb(NO3)2. Lead concentrations in leaves and stems of the accumulating ecotype were 4–9 times and 3–5 times those of the non-accumulating ecotype, and Pb-accumulated amounts in stems and leaves of the accumulating ecotype were 4–9 times and 8–11 times higher than those of the non-accumulating ecotype, respectively. The results indicated that the accumulating ecotype had better ability to transport Pb from roots to shoots. The subcellular distributions of Pb in the root, stem and leaf tissues were studied using sucrose differential centrifugation. Approximately 50% of Pb contents was found to be associated with the cell wall fraction in stems of the accumulating ecotype and the percentage increased to 80% both in roots and leaves, no matter when plants were grown with different levels of Pb. The results indicated that the distribution of Pb on cell walls of the accumulating ecotype could mainly account for the high tolerance to Pb.
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Baker, A. J., McGrath, S. P., Sidoli, C. M. D. and Recves, R. D., 1994. The possibility of in situ heavy metal decontamination of polluted soil using crops of metal-accumulating plants.Resour Conserv. Recycl,11: 41–49.
Blaylock, M. J., Salt, D. E., Dushenkov, S., Zakharova, O., Gussman, C., Kapulnik, Y., Ensley, B. D. and Raskin, I., 1997. Enhanced accumulation of Ph in Indian mustard by soil-applied chelating agents.Environ Sci Technol.,37: 860–865.
Brown, D. H. and Slingsby, D. R., 1972. The cellular location of lead and potassium in the lichen Cladonia rangiformis (L).New Phytol,71: 297–305.
Cunningham, S. D. and Berti, W. R., 1993. Remediation of contaminated soils with green plants: an overview.In Vitro Cell Dev. Biol,29: 207–212.
Cunningham, S. D., Berti, W. R. and Huang, J. W., 1995. Remediation of Contaminated Soils and Sludges by Green Plants.In: Hinchee R. E., Means J. L, Burris D. R., Eds. Bioremediation of inorganics. Batelle Press, Columbus, p. 33–54.
Gabbrielli, R., Panddfini, T., Vergnano, O. and Palandri, N. R., 1990. Comparison of two serpentine species with different nickel tolerance strategies.Plant and Soil,122: 271–277.
He, B., Yang, X. E., Ni, W. Z., Wei, Y. Z., Long, X. X. and Ye, Z. Q., 2002.Sedum alfredii Hance: a new lead-accumulating ecotype.Acta Botanica Sinca,44(11): 1365–1370.
Kumar, P. B. A. N., Dushenkov, V., Motto, H. and Raskin, I., 1995. Phytoextraction: the use of plants to remove heavy metals from soils.Environ Sci Technol,29: 1232–1238.
Macnair, M. R., 1993. The genetics of metal tolerance on vascular plants.New Phytologist,124: 541–559.
Nriagu, J. O., 1992. Toxic metal pollution in Africa.Sci. Total Environ,121: 1–37.
Paff, S. W. and Bosilovich, B. E., 1995. Use of Pb reclamation in secondary lead smelters for the remediation of lead contaminated sites.Journal of Hazardous Materials,40: 139–164.
Raskin, I., Kumar, P. B. A., Dushenkov, S. and Salt, D. E., 1994. Bioconcentration of heavy metals by plants.Curr. Opin. Biotechnol,5: 285–290.
Verkleij, J. A. C. and Schat, H., 1990. Mechanisms of Metal Tolerance in Plants.In: Heavy Metal Tolerance in Plants-Evolutionary Aspects, CRC Press, Boea Raton, FL., p. 179–193.
Weigel, H. J. and Jager, H. J., 1980. Subcellular distribution and chemical form of cadmium in bean.Plant physiol,65: 480–482.
Yang, X. E., Long, X. X., Ni, W. Z. and Ni, S. F., 2001. Zinc tolerance and hyperaccumulation in a new ecotype ofSedum alfredii Hance.Acta Phytoecolgica Scinica,25(6): 670–677.
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Project supported by a Key Project from the Educational Ministry of China (No:02180) and by the Outstanding Young Scientist Grant from the National Natural Science Foundation of China (No: 39925024).
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He, B., Yang, Xe., Ni, Wz. et al. Pb uptake, accumulation, subcellular distribution in a Pb-accumulating ecotype ofSedum alfredii (Hance). J. Zhejiang Univ. Sci. A 4, 474–479 (2003). https://doi.org/10.1631/jzus.2003.0474
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DOI: https://doi.org/10.1631/jzus.2003.0474