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Spermidine affects the transcriptome responses to high temperature stress in ripening tomato fruit

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Abstract

Objective

High temperature adversely affects quality and yield of tomato fruit. Polyamine can alleviate heat injury in plants. This study is aimed to investigate the effects of polyamine and high temperature on transcriptional profiles in ripening tomato fruit.

Methods

An Affymetrix tomato microarray was used to evaluate changes in gene expression in response to exogenous spermidine (Spd, 1 mmol/L) and high temperature (33/27 °C) treatments in tomato fruits at mature green stage.

Results

Of the 10 101 tomato probe sets represented on the array, 127 loci were differentially expressed in high temperature-treated fruits, compared with those under normal conditions, functionally characterized by their involvement in signal transduction, defense responses, oxidation reduction, and hormone responses. However, only 34 genes were up-regulated in Spd-treated fruits as compared with non-treated fruits, which were involved in primary metabolism, signal transduction, hormone responses, transcription factors, and stress responses. Meanwhile, 55 genes involved in energy metabolism, cell wall metabolism, and photosynthesis were down-regulated in Spd-treated fruits.

Conclusions

Our results demonstrated that Spd might play an important role in regulation of tomato fruit response to high temperature during ripening stage.

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Growth, Development and Biotechnology, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou, 310029, China

    Lin Cheng, Rong-rong Sun, Fei-yan Wang, Zhen Peng, Fu-ling Kong, Jian Wu, Jia-shu Cao & Gang Lu

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  1. Lin Cheng
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  2. Rong-rong Sun
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  3. Fei-yan Wang
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  7. Jia-shu Cao
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  8. Gang Lu
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Correspondence to Gang Lu.

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Project supported by the National Basic Research Program (973) of China (No. 2009CB119000), the National Natural Science Foundation of China (Nos. 31071804 and 30771470), and the Zhejiang Provincial Natural Science Foundation (Nos. R3110209 and 2009C32025), China

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Cheng, L., Sun, Rr., Wang, Fy. et al. Spermidine affects the transcriptome responses to high temperature stress in ripening tomato fruit. J. Zhejiang Univ. Sci. B 13, 283–297 (2012). https://doi.org/10.1631/jzus.B1100060

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