Various types of zeolites were evaluated for the adsorptive separation of ethanol and gasoline in ethanol blended gasoline. Mixed solution of ethanol and n-heptane was used as a model fuel. Adsorption selectivities of zeolites for ethanol and n-heptane were measured by a pulse testing method at 80 °C. These properties were dependent on the framework structures, exchangeable cations and Si/Al atomic ratios of zeolites. The adsorption capacities of zeolites for ethanol and n-heptane were evaluated by a continuous flow adsorption testing method at 80 °C. Zeolites with the FAU type structure (Na-Y and H-Y) showed higher adsorption capacities compared to the other types of zeolites. Adsorption strength of ethanol on zeolites was evaluated by a temperature programmed desorption method. Ethanol molecules adsorbed on Na-Y and H-Y were desorbed by heat treatment at around 300 °C, but ethanol adsorbed on H-Y was partially dehydrated because of zeolite acidity.