Phylogenetic Relationships in Ephedra (Gnetales): Evidence from Nuclear and Chloroplast DNA Sequence Data
Sequences from the nuclear ribosomal internal transcribed spacer region 1 (nrDNA ITS1) and the plastid rps4 gene from the genus Ephedra (Ephedraceae, Gnetales) were obtained in order to infer phylogenetic relationships, character evolution, and historical biogeography
in the genus. Within Ephedra the length of the nrDNA ITS1 varied from 1,081 to 1,143 basepairs (bp), in contrast to dramatically shorter lengths in the outgroups (Gnetum, Welwitschia, and Pinus). The rps4 locus varied in length from 645 to 661 bp in the
same set of taxa. Both parsimony and maximum likelihood analyses of these sequences resulted in a well-resolved phylogeny that supports the monophyly of Ephedra, but not its subdivision into the traditional sections Ephedra, Asarca, and Alatae. The resulting phylogeny
also indicates a derivation of the New World clade from among the Old World taxa. Among the Old World species three highly-supported monophyletic groups are recognized that are highly concordant with morphological evidence. The New World clade includes two main subclades of North and South
American species that are strongly supported, while the position of two, mostly Mexican species E. pedunculata and E. compacta remains unresolved. Character reconstruction of ovulate strobilus types in Ephedra indicates that fleshy bracts are ancestral, with shifts to
dry, winged bracts having occurred multiple times. Low levels of sequence divergence within the North American clade suggest either recent and rapid ecological radiation or highly conservative ribosomal DNA evolution within the clade.
Document Type: Regular Paper
Publication date: 01 October 2004
- Systematic Botany is the scientific journal of the American Society of Plant Taxonomists and publishes four issues per year.
2011 Impact Factor: 1.517
2011 ISI Journal Citation Reports® Rankings: 87/190 - Plant Sciences
34/45 - Evolutionary Biology - Editorial Board
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