Citation:

PANGAEA.924440

Name: Results of a 24-hour laboratory experiment sampling cell concentrations, particulate organic carbon (POC), particulate inorganic carbon (PIC) and further cell parameters of the coccolithophore Emiliania huxleyi grown under a 16:8 h light/dark cycle
Published: 2020-11-04
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Exponential growth; Light/dark cycles; Phased cell division; PIC production; POC production

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Abstract:

Cell division of the coccolithophore Emiliania huxleyi and other phytoplankton typically becomes entrained to diel light/dark cycles under laboratory conditions, with division occurring primarily during dark phases and production occurring during light phases. Under these conditions, the increase in a culture's cell and biomass concentrations deviates from an exponential function on time scales < 24 h. We here present a dataset of short-term changes in cell and biomass concentrations of fast dividing, dilute-batch cultures of E. huxleyi grown under a 16:8 h light/dark cycle. This dataset was used to derive linear models describing the diel course in the concentrations of cells, particulate organic carbon (POC) and particulate inorganic carbon (PIC) and for the calculation of daily means of cellular quotas and production rates in Kottmeier et al. (2020). We also present the given seawater carbonate chemistry as well as cellular quotas of particulate organic nitrogen (PON) and chlorophyll a (Chl. a), and the ratios of PIC:POC, POC:PON, POC:cell volume and Chl. a:POC in the course of the 24 h sampling period.

Keyword(s):

Exponential growth; Light/dark cycles; Phased cell division; PIC production; POC production

Related to:

Kottmeier, Dorothee; Terbrüggen, Anja; Wolf-Gladrow, Dieter A; Thoms, Silke (2020): Diel variations in cell division and biomass production of Emiliania huxleyi - Consequences for the calculation of physiological cell parameters. Limnology and Oceanography, 65(8), 1781-1800, https://doi.org/10.1002/lno.11418

Funding:

European Research Council (ERC), grant/award no. 205150: SEACELLS - Marine phytoplankton as biogeochemical drivers: Scaling from membranes and single cells to populations

Federal Ministry of Education and Research (BMBF), grant/award no. 031A518C: ZeBiCa2: Zellfreie Biomineralisation

Coverage:

Latitude: -42.180000 * Longitude: 169.500000

Date/Time Start: 1998-10-17T23:30:00 * Date/Time End: 1998-10-17T23:30:00

Minimum Elevation: 0.0 m * Maximum Elevation: 10.0 m

Event(s):

SO136_006-A_HPN (1) * Latitude: -42.180000 * Longitude: 169.500000 * Date/Time: 1998-10-17T23:30:00 * Elevation Start: 0.0 m * Elevation End: 10.0 m * Campaign: (TASQWA) * Basis: * Method/Device: Hand net (HN)

Comment:

Relative concentrations refer to concentrations relative to initial (t0) concentrations.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Cell concentration, relative	Cell conc rel		Kottmeier, Dorothee	Coulter counter, Beckman Coulter, Multisizer 3	#/ml (#/ml)t0-1
2	Species	Species		Kottmeier, Dorothee
3	Registration number of species	Reg spec no		Kottmeier, Dorothee
4	Uniform resource locator/link to reference	URL ref		Kottmeier, Dorothee		WoRMS Aphia ID
5	Strain	Strain		Kottmeier, Dorothee
6	Uniform resource locator/link to reference	URL ref		Kottmeier, Dorothee		Roscoff Culture Collection
7	Stage	Stage		Kottmeier, Dorothee		Life cycle stage
8	Irradiance	E	µmol/m²/s	Kottmeier, Dorothee	Light meter (LM)
9	Treatment: light:dark cycle	T:L:D	hh:hh	Kottmeier, Dorothee
10	Growth rate	µ	1/day	Kottmeier, Dorothee	Coulter counter, Beckman Coulter, Multisizer 3
11	Growth rate, standard deviation	µ std dev	±	Kottmeier, Dorothee	Coulter counter, Beckman Coulter, Multisizer 3
12	Salinity	Sal		Kottmeier, Dorothee	Salinometer (SALINO)
13	Temperature, water	Temp	°C	Kottmeier, Dorothee	Temperature sensor
14	pH	pH		Kottmeier, Dorothee	Potentiometric	NBS scale
15	pH, standard deviation	pH std dev	±	Kottmeier, Dorothee	Potentiometric	NBS scale
16	Alkalinity, total	AT	µmol/kg	Kottmeier, Dorothee	Alkalinity, potentiometric	Dickson-corrected
17	Alkalinity, total, standard deviation	AT std dev	±	Kottmeier, Dorothee	Alkalinity, potentiometric	Dickson-corrected
18	Carbon, inorganic, dissolved	DIC	µmol/kg	Kottmeier, Dorothee	Colorimetric autoanalysis
19	Consumption of carbon, inorganic, dissolved, standard deviation	DIC consumpt std dev	±	Kottmeier, Dorothee	Colorimetric autoanalysis
20	Carbon dioxide, partial pressure	pCO2	µatm	Kottmeier, Dorothee	Calculated using CO2SYS
21	Carbon dioxide, partial pressure, standard deviation	pCO2 std dev	±	Kottmeier, Dorothee	Calculated using CO2SYS
22	Identification	ID		Kottmeier, Dorothee		Culture ID
23	Time in hours	Time	h	Kottmeier, Dorothee		within dark:light cycle
24	Phase	Phase		Kottmeier, Dorothee
25	Cell concentration, relative	Cell conc rel		Kottmeier, Dorothee	Coulter counter, Beckman Coulter, Multisizer 3	#/ml (#/ml)t0-1
26	Carbon, organic, particulate, relative concentration	POC conc rel		Kottmeier, Dorothee	Elemental analyzer, EuroVector, EA 3000	g/ml (g/ml)t0-1
27	Carbon, inorganic, particulate, relative concentration	PIC conc rel		Kottmeier, Dorothee	Elemental analyzer, EuroVector, EA 3000	g/ml (g/ml)t0-1
28	Carbon, organic, particulate, per cell	POC/cell	pg/#	Kottmeier, Dorothee	Elemental analyzer, EuroVector, EA 3000
29	Carbon, inorganic, particulate, per cell	PIC/cell	pg/#	Kottmeier, Dorothee	Elemental analyzer, EuroVector, EA 3000
30	Particulate organic nitrogen per cell	PON/cell	pg/#	Kottmeier, Dorothee	Elemental analyzer, EuroVector, EA 3000
31	Chlorophyll a per cell	Chl a/cell	pg/#	Kottmeier, Dorothee	Fluorometer, Turner Design, TD-700
32	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Kottmeier, Dorothee		mol/mol
33	Carbon, organic, particulate/Nitrogen, organic, particulate ratio	POC/PON	mol/mol	Kottmeier, Dorothee
34	Carbon, organic, particulate	POC	fg/µm³	Kottmeier, Dorothee		per cell volume
35	Chlorophyll a/particulate organic carbon ratio	Chl a/POC		Kottmeier, Dorothee		g/g

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

1983 data points

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