Trypanosoma cruzi: cell charge distribution

Langenbach, T.

doi:10.1590/S0036-46651985000600004

Abstracts

Differences in cell charge between epimastigote and trypomastigote populations were compared in Y, Cl and Colombiana strains of T. cruzi. Trypomastigote populations were more homogenous in relation to cell charge than epimastigotes. This homogeneity of cell charge was not the result of the selection of trypomastigote sub-populations by the host immunosystem, but may be the result of a surface coat formed by host blood components.

Diferenças na carga celular entre populações de epimastigotas e de tripomastigotas foram comparadas nas cepas Y, CL e Colombiana do T. cruzi. As populações de tripomastigotas mostraram-se mais homogêneas quanto à carga celular do que os epimastigotas. Esta maior homogeneidade de cargas não foi decorrente da seleção de sub-populações de tripomastigotas por ação do sistema imunológico do hospedeiro, mas talvez esta se deva à capa superficial formada por componentes sangüíneos dos hospedeiros.

ORIGINAL ARTICLES

Trypanosoma cruzi: cell charge distribution

Trypanosoma cruzi: distribuição de cargas celulares

T. Langenbach

Instituto de Microbiologia da Universidade Federal do Rio de Janeiro, Departamento de Microbiologia Geral, Bloco I. Cidade Universitária, Ilha do Fundão, 21.944 Rio de Janeiro, RJ, Brazil

SUMMARY

Differences in cell charge between epimastigote and trypomastigote populations were compared in Y, Cl and Colombiana strains of T. cruzi. Trypomastigote populations were more homogenous in relation to cell charge than epimastigotes. This homogeneity of cell charge was not the result of the selection of trypomastigote sub-populations by the host immunosystem, but may be the result of a surface coat formed by host blood components.

RESUMO

Diferenças na carga celular entre populações de epimastigotas e de tripomastigotas foram comparadas nas cepas Y, CL e Colombiana do T. cruzi. As populações de tripomastigotas mostraram-se mais homogêneas quanto à carga celular do que os epimastigotas. Esta maior homogeneidade de cargas não foi decorrente da seleção de sub-populações de tripomastigotas por ação do sistema imunológico do hospedeiro, mas talvez esta se deva à capa superficial formada por componentes sangüíneos dos hospedeiros.

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ACKNOWLEDGEMENTS

This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Conselho de Ensino e Pesquisa da Universidade Federal do Rio de Janeiro (CEPG-UFRJ), and Financiadora de Estudos e Projetos (FINEP). I would like to thank Dr. L.R. Travassos and Dr. A.N. Hagler for their helpful criticisms and Dr. F.S. Cruz and M.A. Sousa for the supply of T. cruzi strains. The skillful technical assistance of M.F. de Araújo is gratefully acknowledged.

Recebido para publicação em 10/7/1984.

1. AL-ABBASSY, S. N.; SEED, T, M. & KREIER, J. P. Isolation of the trypomastigote form of Trypanosoma cruzi from a mixture of the trypomastigote and epimastigote forms of the parasite by use of a DEAE-cellulose column. J. Parasitol. 58: 631-632, 1972.
2. ALVES, M. J. M. & COLLI, W. Agglutination of Trypanosoma cruzi by Concanavalin A. J. Protozool. 21: 574-578, 1974.
3. BRENNER, Z. Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi. Rev. Inst. Med. trop. São Paulo 4: 389-396, 1962.
4. BRENNER, Z. & CHIARI, E. Variações morfológicas observadas em diferentes amostras de Trypanosoma cruzi. Rev. Inst. Med. trop. São Paulo 5: 128-132, 1963.
5. BORST, P. & CROSS, G. A. Molecular basis for trypanosome antigenic variation. Cell 29: 291-303, 1982.
6. CROSS, G. A. M. Antigenic variation in Trypanosomas. Proc. Roy. Soc. London (Ser. B) 202: 55-72, 1978.
7. DE SOUZA, W.; ARGUELLO, C; MARTINEZ-PALOMO, A.; TRISSL, D.; GONZALEZ-ROBLES, A. & CHIARI, E. Surface charge of Trypanosoma cruzi. Binding of cationized Ferritin and measurement of cellular electrophoretic mobility. J. Protozool. 24: 411-415, 1977.
8. DE SOUZA, W.; MARTINEZ-PALOMO, A. & GONÇALEZ-ROBLES, A. The cell surface of Trypanosoma cruzi: cytochemistry and freeze-fracture. J. Cell Sci. 33: 285-299, 1978.
9. PEDERICI, E. E.; ABELMANN, W. B. & NEVA, F. A. Chronic and progressive myocarditis and myositis in C_aH mice infected with Trypanosoma cruzi. Amer. J. Trop. Med. Hyg. 13: 272-280, 1964.
10. GUTTERIDGE, W. E.; COVER, B. & GABORAK, M. Isolation of blood and intracellular forms of Trypanosoma cruzi from rats and other rodents and preliminary studies of their metabolism. Parasitology 76: 156-176, 1976.
11. HOARE, C. A. & WALLACE, P. C. Developmental stages of trypomastigote flagellates. A new terminology. Nature (London) 212: 1385-1386, 1966.
12. KREIER, J. P.; AL-ABBASSY, S. N. A. & SEED, T. M. Trypanosoma cruzi; surface change characte- ristics of cultured epimastigotes trypomastigotes and amastigotes. Rev. Inst. Med. trop. São Paulo 19: 10-20, 1977.
13. KRETTLI, A. & NUSSENZWEIG, R. S. Presence of immunoglobulins of the surface of circulating trypomastigotes of Trypanosoma cruzi resulting in activation of the alternative pathway of complement and lysis. PAHO, Dis. SC Publ. 347: 71-73 1977.
14. LANHAM, S. M. Separation on infected rats and mice by anion-exchanges. Nature (London) 218: 1273-1274, 1968.
15. LANHAM, S. M. & GODFREY, D. G. Isolation of salivarian Trypanosomes from man and others mammals using DEAE-cellulose. Exp. Parasitol. 28: 521-534, 1970.
16. MERCADO, T. T. & KATUSHA, K. Infection of Trypanosoma cruzi from the blood of infected mice by column chromatography. Preparative Biochem. 9: 97-106, 1979.
17. PEREIRA, M. E. A.; LOURES, M. A.; VILLALTA, F. & ANDRADE, A. F. B. Lectin receptors as markers for Trypanosoma cruzi Developmental stages and a study of the interaction of wheat germ agglutinin with sialic acid residues on epimastigote cells. J. Exp. Med. 152: 1375-1392, 1980.
18. SILVA, L. H. P. & NUSSENZWEIG, V. An strain of Trypanosoma cruzi higly virulent for white mice. Folia Clin. Biol. 20: 191-207, 1953.
19. SOUSA, M. A. Novo método de purificação de tripomastigotas de diferentes cepas de Trypanosoma cruzi em colunas de DEAE-celuIose a partir do sangue de camundongos. Biologia e antigenicidade dos parasitos purificados. Dissertation. 1981.
20. TURNER, M. How Trypanosomes change coats. Nature (London) 284: 13-14, 1980.
21. VICKERMAN, K. Antigenic variation in Trypanosomes. Nature (London) 273: 613-617, 1978.
22. VILLALTA, F. & LEON, W. Effect of purification by DEAE-cellulose column on infectivity of Trypanosoma cruzi blood forms. J. Parasitol. 65: 188-189, 1979.
23. WARREN, L. G. Metabolism of Schizotrypanum cruzi Chagas. I. Effect of culture age and substrate concentration on respiratory rate. J. Parasitol. 46: 529-539, 1960.
24. ZENIAN, A. & KIERZENBAUM, F. Trypanosoma cruzi: differences in cell surface interaction of circulating (Trypomastigote) and culture (epimastigote) forms with macrophages. J. Parasitol. 69: 665-670, 1983.

Publication Dates

Publication in this collection
25 Oct 2012
Date of issue
Dec 1985

History

Received
10 July 1984

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. AL-ABBASSY, S. N.; SEED, T, M. & KREIER, J. P. Isolation of the trypomastigote form of Trypanosoma cruzi from a mixture of the trypomastigote and epimastigote forms of the parasite by use of a DEAE-cellulose column. J. Parasitol. 58: 631-632, 1972.

[2] 2. ALVES, M. J. M. & COLLI, W. Agglutination of Trypanosoma cruzi by Concanavalin A. J. Protozool. 21: 574-578, 1974.

[3] 3. BRENNER, Z. Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi. Rev. Inst. Med. trop. São Paulo 4: 389-396, 1962.

[4] 4. BRENNER, Z. & CHIARI, E. Variações morfológicas observadas em diferentes amostras de Trypanosoma cruzi. Rev. Inst. Med. trop. São Paulo 5: 128-132, 1963.

[5] 5. BORST, P. & CROSS, G. A. Molecular basis for trypanosome antigenic variation. Cell 29: 291-303, 1982.

[6] 6. CROSS, G. A. M. Antigenic variation in Trypanosomas. Proc. Roy. Soc. London (Ser. B) 202: 55-72, 1978.

[7] 7. DE SOUZA, W.; ARGUELLO, C; MARTINEZ-PALOMO, A.; TRISSL, D.; GONZALEZ-ROBLES, A. & CHIARI, E. Surface charge of Trypanosoma cruzi. Binding of cationized Ferritin and measurement of cellular electrophoretic mobility. J. Protozool. 24: 411-415, 1977.

[8] 8. DE SOUZA, W.; MARTINEZ-PALOMO, A. & GONÇALEZ-ROBLES, A. The cell surface of Trypanosoma cruzi: cytochemistry and freeze-fracture. J. Cell Sci. 33: 285-299, 1978.

[9] 9. PEDERICI, E. E.; ABELMANN, W. B. & NEVA, F. A. Chronic and progressive myocarditis and myositis in C_aH mice infected with Trypanosoma cruzi. Amer. J. Trop. Med. Hyg. 13: 272-280, 1964.

[10] 10. GUTTERIDGE, W. E.; COVER, B. & GABORAK, M. Isolation of blood and intracellular forms of Trypanosoma cruzi from rats and other rodents and preliminary studies of their metabolism. Parasitology 76: 156-176, 1976.

[11] 11. HOARE, C. A. & WALLACE, P. C. Developmental stages of trypomastigote flagellates. A new terminology. Nature (London) 212: 1385-1386, 1966.

[12] 12. KREIER, J. P.; AL-ABBASSY, S. N. A. & SEED, T. M. Trypanosoma cruzi; surface change characte- ristics of cultured epimastigotes trypomastigotes and amastigotes. Rev. Inst. Med. trop. São Paulo 19: 10-20, 1977.

[13] 13. KRETTLI, A. & NUSSENZWEIG, R. S. Presence of immunoglobulins of the surface of circulating trypomastigotes of Trypanosoma cruzi resulting in activation of the alternative pathway of complement and lysis. PAHO, Dis. SC Publ. 347: 71-73 1977.

[14] 14. LANHAM, S. M. Separation on infected rats and mice by anion-exchanges. Nature (London) 218: 1273-1274, 1968.

[15] 15. LANHAM, S. M. & GODFREY, D. G. Isolation of salivarian Trypanosomes from man and others mammals using DEAE-cellulose. Exp. Parasitol. 28: 521-534, 1970.

[16] 16. MERCADO, T. T. & KATUSHA, K. Infection of Trypanosoma cruzi from the blood of infected mice by column chromatography. Preparative Biochem. 9: 97-106, 1979.

[17] 17. PEREIRA, M. E. A.; LOURES, M. A.; VILLALTA, F. & ANDRADE, A. F. B. Lectin receptors as markers for Trypanosoma cruzi Developmental stages and a study of the interaction of wheat germ agglutinin with sialic acid residues on epimastigote cells. J. Exp. Med. 152: 1375-1392, 1980.

[18] 18. SILVA, L. H. P. & NUSSENZWEIG, V. An strain of Trypanosoma cruzi higly virulent for white mice. Folia Clin. Biol. 20: 191-207, 1953.

[19] 19. SOUSA, M. A. Novo método de purificação de tripomastigotas de diferentes cepas de Trypanosoma cruzi em colunas de DEAE-celuIose a partir do sangue de camundongos. Biologia e antigenicidade dos parasitos purificados. Dissertation. 1981.

[20] 20. TURNER, M. How Trypanosomes change coats. Nature (London) 284: 13-14, 1980.

[21] 21. VICKERMAN, K. Antigenic variation in Trypanosomes. Nature (London) 273: 613-617, 1978.

[22] 22. VILLALTA, F. & LEON, W. Effect of purification by DEAE-cellulose column on infectivity of Trypanosoma cruzi blood forms. J. Parasitol. 65: 188-189, 1979.

[23] 23. WARREN, L. G. Metabolism of Schizotrypanum cruzi Chagas. I. Effect of culture age and substrate concentration on respiratory rate. J. Parasitol. 46: 529-539, 1960.

[24] 24. ZENIAN, A. & KIERZENBAUM, F. Trypanosoma cruzi: differences in cell surface interaction of circulating (Trypomastigote) and culture (epimastigote) forms with macrophages. J. Parasitol. 69: 665-670, 1983.

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Trypanosoma cruzi: cell charge distribution

Trypanosoma cruzi: distribuição de cargas celulares

Abstracts

Publication Dates

History