Time series HPLC pigment data as measured by Horn Point Laboratory (HPL) from B/O Hermano Gines cruises CAR-124 to CAR-175 in the CARIACO basin from 2006-07-04 to 2010-12-08 (CARIACO Ocean Time-Series Program)

The CARIACO Ocean Time-Series Program (formerly known as CArbon Retention In A Colored Ocean) started on November 1995 (CAR-001) and ended on January 2017 (CAR232). Throughout the CARIACO time-series, High Performance Liquid Chromatography (HPLC) data was analyzed by four different laboratories: Bermuda Biological Research Station; Mote Marine Laboratory; Horn Point Laboratory; and NASA Goddard Space Flight Center. This package contains the data analyzed at Horn Point Laboratory, covering cruises CAR-124 to CAR-175 from 2006-07-04 to 2010-12-08. Some of the parameters analyzed were different along time and along the different laboratories. To keep the continuity of the HPLC time-series analyzed by different laboratories, all the HPLC files have the same units and contain the same number and order of columns/parameters (with “nd” to indicate when a parameter was no determined). Fluorometric Chlorophyll-a and Phaeopigments (measured at Estación de Investigaciones Marinas de Margarita, Fundación La Salle, EDIMAR-FLASA) are also included. HPLC was not analyzed for cruises CAR-069 to CAR-123. A general description of the CARIACO Ocean Time-Series Program can be found at www.imars.usf.edu/cariaco.

contain the same number and order of columns/parameters (with "nd" to indicate when a parameter was no determined). Fluorometric Chlorophyll-a and Phaeopigments (measured at Estación de Investigaciones Marinas de Margarita, Fundación La Salle, EDIMAR-FLASA) are also included. HPLC was not analyzed for cruises CAR-069 to CAR-123. A general description of the CARIACO Ocean Time-Series Program can be found at www.imars.usf.edu/cariaco. www.bco-dmo.org/dataset/3293

Table of Contents
These data were also funded by the following awards: -23914: Ley Orgánica de Ciencia, Tecnología e Innovación, LOCTI (Estación de Investigaciones Marinas), Venezuela.

Acquisition Description
Water for the HPLC filters was collected from the first cast done during the CARIACO core cruises, carried out around 5 am local time. During periods of low primary production, 2000 ml.
of water were vacuum filtered through a 47mm GF/F filter pad. During periods of high primary production, water was filtered until the filter was clogged. The volume of water filtered was annotated. Eight depths were sampled: 1,7,15,25,35,55,75 and 100 m. If a chlorophyll maximum was found at a different depth, this depth was also sampled. A duplicate was taken at one of these depths (usually at 1m), for QA/QC assessment. Filters were carefully folded in half, stored in aluminum foil and refrigerated until reaching shore. Once back on shore, they were stored frozen at -40°C. Filters were transported from Margarita Island to USF and stored frozen (at -40°C) until they were shipped in a liquid-nitrogen storage to the laboratory that The HPLC parameter names as reported in this dataset correspond to the pigment names as defined in the NASA SeaWiFS Bio-optical Archive and Storage System (SeaBASS) (https://seabass.gsfc.nasa.gov/wiki/stdfields). Fluorometric estimations of chlorophyll-a and phaeopigments concentrations were done with a Turner Design10-AU-005 Fluorometer using standard methods (Holm-Hansen et al., 1965;Falkowski and Kiefer, 1985  [ Basin. This depression, located on the continental shelf of Venezuela (Map), shows marked seasonal and interannual variation in hydrographic properties and primary production (carbon fixation rates by photosynthesis of planktonic algae). This peculiar basin is anoxic below ~250 m, due its restricted circulation and high primary production (Muller-Karger et al., 2001).

Related Publications
CARIACO observations show annual primary production rates exceed 500 gC/m2y, of which over 15-20% can be accounted for by events lasting one month or less. Such events are observed in other locations where time series observations are collected, and suggest that prior estimates of regional production based on limited sampling may have been underestimated. The annual primary production rates in the Cariaco Basin are comparable to rates estimated using time series observations for Monterey Bay (460 gC/m2y;Chavez, 1996), and higher than previous rates estimated for Georges Bank, the New York Shelf, and the Oregon Shelf (380, 300, and 190 gC/m2y, respectively;Walsh, 1988 , 1999;Peterson et al., 2000;Haug et al., 2001;Black et al., 2004;Hughen et al., 2004 ). As we studied ocean biogeochemistry, we learned that our simple views of carbon uptake and transport were severely limited, and a new "wave" of ocean science was born. U.S. JGOFS