Hand Hygiene Compliance among Healthcare Workers in Public-Sector Rural Hospitals in Benin

Introduction Hand Hygiene Compliance among Healthcare Workers in Public-Sector Rural Hospitals in Benin Dégbey C *1,2, Glèlè Ahanhanzo Y1, Kakikatsoma TK1, Ouendo EM1 and Makoutode M1 1Regional Institute of Public Health, University of Abomey-Calavi, Ouidah, Benin 2University Hospital Hygiene Clinic, National University Hospital Center Hubert Koutoukou MAGA, Cotonou, Benin *Corresponding author: Dégbey C, Regional Institute of Public Health, University of Abomey-Calavi, Ouidah, Benin, Tél. 0022995960541; E-mail: comlancy@yahoo.fr

Healthcare workers therefore need to improve their hand hygiene in order to reduce the prevalence of healthcare-associated infections. To effectively achieve this, an understanding of the context is required along with an analysis of the factors behind the current situation. This study aims to examine the factors associated with hand hygiene compliance among healthcare workers in a rural district hospital in Benin.
This study was conducted in the hospital in Aplahoué health district in the department of Kouffo in Benin. It is a district referral hospital that covers an essentially rural population of 250,000. The hospital has the following healthcare departments: emergency and intensive care department, maternity department, general medicine department, ophthalmology department, surgical department, physiotherapy department, operating theatres, a laboratory and the radiology department.
The study was a cross-sectional descriptive and analytical study conducted in 2017.
The primary targets were healthcare workers carrying out healthcare activities in the hospital's departments. A non-probability sampling method was used with an exhaustive technique. The sample comprised 59 subjects, broken down as follows: four (04) doctors, twenty (20) nurses, four (04) midwives, five (05) biomedical technicians, one (01) anaesthetist nurse and twenty-five (25) auxiliary nurses. The latter are an occupational category that is not trained in a professional school but which supports healthcare providers in their day-to-day activities.
Laboratory technicians in the performance of their duties of service (blood, vaginal, throat, blood culture and others) should perform hand hygiene (simple hand washing, hygienic hand washing and friction hands) to not transmit to patients infections associated with care and services.
The techniques used for data collection were a survey questionnaire and direct observation during the provision of healthcare or services. The collection tools developed and used were a questionnaire and an observation chart. To assess the knowledge of those surveyed we used the WHO questionnaire on hand hygiene for healthcare workers [1]. This questionnaire has 11 questions with 36 possible correct answers. The questions are multiple-choice questions (MCQ); for each question there are a series of answers from which the healthcare worker chooses the one or ones they think correct. A score of "0" was given for negative attitudes and poor practices. A score of "1" was given for each correct answer associated with positive attitudes and good practices, meaning that the maximum score was 36. A scale was used to grade the healthcare workers according to their level of knowledge. A total score for a healthcare worker ≥ 75% was considered "good", between 50 and 74% "moderate" and 50% "Insufficient".
Hand hygiene compliance was assessed based on practice, using an observation chart that covered: i) the type of healthcare requiring hand hygiene; ii) the type of hand hygiene applied; iii) the procedure for washing hands with soap and water; iv) the factors associated with the "standard precautions" for hand washing; v) the opportunities and vi) the actions based on the WHO's five moments for hand hygiene. A "compliance rate" for each healthcare worker was calculated by comparing the number of actions carried out for each type of care that required hand hygiene and the number of opportunities.
The dependent variable was hand hygiene compliance among healthcare workers working in one of the five buildings of the Aplahoué district hospital, expressed as the "compliance rate". The independent variables were associated with: Socio-demographic characteristics and background; age, sex, occupational category, seniority in the profession, soap allergies.

Data Collection Techniques, Tools and Measures
Variables Journal of Public Health, Hygiene and Safety The data was entered using the software Epi Info version 7, and analysed using Stata 11. Proportion comparisons were conducted using the chi-square test. For the search for links between hand hygiene compliance and factors associated with the healthcare workers, a univariate analysis of the data by simple linear regression was conducted using the Fisher statistical test with a p-value considered significant at a threshold of 5%.

Data Processing and Statistical Analysis
In total, 59 healthcare workers with a median age of 38 (30-42) participated in the study, of which 38 (64.4%) were women and 25 (42%) auxiliary nurses ( Table 1).

Characteristics
Number 76.3% of the healthcare workers have a moderate knowledge of hand hygiene (Table 2), 62.7% know the main way germs are spread between patients in a healthcare facility, and 93.2% know the components of hand hygiene.
The healthcare workers were observed for 30 to 60 minutes as they delivered care or services, excluding major emergency situations. Out of the 963 opportunities counted, 117 actions were recorded during the provision of the healthcare or service and all involved simple washing with soap and water, giving an overall compliance rate of 12.15%. It varies significantly according to the World Health Organisation's five indications between 2.1% prior to touching the patient and 50% in cases of exposure to a biological fluid (p<0.000) (Figure 1). This rate also varies significantly according to the department of activity, with a higher compliance rate in the laboratory (p-value=0.008) in relation to the general medicine department.    The individual hand hygiene compliance rate among the healthcare workers at the Aplahoue district hospital varies between 0% and 40%.

Level of Hand Hygiene and Hospital-Acquired Infection
The factors related to health professionals associated with hand hygiene compliance were knowledge of hospital-acquired infections from basic training (coefficient= 8.86 and p-value =0.000), knowledge of hand hygiene in hospital settings from basic training (coefficient=8.25 and p-value=0.001), soap allergies (coefficient= -5.46 and p-value = 0.041), and occupational category (p-value < 0.05) (Table 3a).
Regarding the factors related to health professionals in relation to the standard precautions related to the rate of compliance of hand hygiene, only the wearing short-sleeved scrubs (coefficient= 9.82 and p-value = 0.011) ( This study conducted on 59 healthcare workers provides a point-in-time analysis of the situation. It highlights the issue of hand hygiene knowledge and compliance in our hospitals. The proportion of healthcare workers with a "good" level of awareness, 23.73% (14/59), is higher than the 9% (13/144) found in India by Nair SS et al. [7]. This difference can be explained by the difference in the occupational category of those surveyed. Nair SS et al. conducted their study among medical and nursing students. The proportion of healthcare workers who knew the components of hand hygiene were 93.22%, compared with the 0% found by Hien H et al. in their study in Burkina Faso in 2013 [8]. This difference can be explained by the techniques used to collect the data from the healthcare workers in our different studies. Those who know the main way germs are spread between patients in a healthcare facility represented 62.71% in our study, and a score of 44.6% in the study by Hien H et al., but these scores were still lower than the 73.61% found by Nair SS et al. [7,8]. The overall hand hygiene compliance rate among healthcare workers delivering healthcare and services in the Aplahoue district hospital was very low in 2017. Due to the quality of their professional training, auxiliary nurses may have less knowledge concerning hygiene before and after providing healthcare; as a consequence, they need more support with hand hygiene practices and compliance in hospital settings than those from other occupational categories. The overall hand hygiene compliance rate found by Uneke CJ et al. in their study on the promotion of hand hygiene in 2014 in Nigeria was six (06) times higher than that found in our study, in other words 65.3% [10]. This high rate in Nigeria is warranted as the study was divided into two phases: an intervention phase (training for doctors and nurses, use of reminders concerning hand hygiene in the form of posters in strategic places around the hospital, and the provision of bottles of 70% alcohol isopropyl) and an evaluation phase two months after the intervention. It is normal therefore that there should be an improvement in hand hygiene compliance. The 50% compliance rate after exposure to a biological fluid in our study was higher than that found by Karaaslan A et al. (18.10%) in their study conducted in Istanbul in 2014 [9]. Our study revealed compliance rates of 2.07% "before touching a patient" and 21.93% "after touching a patient"; these results were lower than those obtained by Diarra F in Benin in 2007: 67.1% and 91.2% before and after the medical intervention respectively [11]. This study was conducted on 362 healthcare workers at Cotonou hospital, a referral hospital with an operational hospital hygiene department and which therefore supports and supervises staff in the area of hospital hygiene on a day-to-day basis; which is not the case at the Aplahoue district hospital. The setting of the study would therefore explain this difference.

Discussion
In this study, the factors associated with the hand hygiene compliance rate were identified. As such, there is a significant link between the overall hand hygiene compliance rate and knowledge of hospital-acquired infections from basic training. The average overall hand hygiene compliance rate is lower (by approximately 8.86%) among healthcare workers who do not have knowledge of hospital-acquired infections from their basic training in relation to the average overall hand hygiene compliance rate among healthcare workers who do have knowledge of hospital-acquired infections from their basic training. Likewise, there is a significant link between the overall hand hygiene compliance rate and knowledge of hand hygiene in hospital settings from basic training. The average overall hand hygiene compliance rate is lower (by approximately 8.25%) among healthcare workers who do not have knowledge of hand hygiene in hospital settings from their basic training in relation to the average overall hand hygiene compliance rate among healthcare workers who do have knowledge of hand hygiene in hospital settings from their basic training. It is therefore important to introduce teaching on hospital-acquired infections and hospital hygiene in basic training courses in the country's schools and faculties in the health sciences sector.
As regards soap allergies, there is a significant link between the overall hand hygiene compliance rate and soap allergies. Indeed, the average overall hand hygiene compliance rate is higher (by approximately 5.46%) among healthcare workers who are not allergic to soap in relation to the average overall hand hygiene compliance rate among professionals who are allergic to soap. Hydro-alcohol rubbing is a valid alternative that should be considered.
As regards compliance with standard precautions recommended to prevent hospital-acquired infections, there is a significant link between the overall hand hygiene compliance rate and the wearing of short-sleeved scrubs. The average overall hand hygiene compliance rate is in fact higher (by approximately 9.82%) among healthcare workers who wear short-sleeved scrubs in relation to the average overall hand hygiene compliance rate among healthcare workers who do not wear short-sleeved scrubs ( Although not found as factors associated with the low rate of hand hygiene compliance in this study, capacity building on hospitalacquired infections in the last 3 years and on hand hygiene in hospital settings in the last 3 years, and current training remain major factors in the strategy to promote hand hygiene in hospital settings [12].
After the study, there were recommendations that have already been made in the direction of the hospital on the in-service training in hospital hygiene of the nursing staff and posters of communication for a change of behaviour and the use almost permanent 70% alcohol for hand rub.
Similar studies should be undertaken at the level of two health departments expanding the study framework by including at least three zone hospitals. These studies may identify other factors that may be associated with observance of hand hygiene in hospitals. Journal of Public Health, Hygiene and Safety The non-probabilistic method, the exhaustiveness technique and the small sample size could not make it possible to generalize the results obtained to all health facilities in the region.
Our study shows the factors that determine the hand hygiene compliance rate. These are: knowledge of hospital-acquired infections, and knowledge of hand hygiene in hospital settings from basic training. The wearing of short-sleeved scrubs, soap allergies and occupational category are also factors associated with hand hygiene compliance. The current level of knowledge of hand hygiene among healthcare workers is acceptable. The overall hand hygiene compliance rate remains low (12.15%). A multimodal strategy should be undertaken to increase this rate and help improve hospital hygiene in the Aplahoue district hospital.