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Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels

  • Biomaterials for 3D Cell Biology Research Letter
  • Published:
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Abstract

Human mesenchymal stem cells (MSCs) are the most intensely studied and clinically used adult stem cell type. Conventional long-term cultivation of MSCs as a monolayer is known to result in a reduction of their functionality and viability. In addition, large volumes of cell culture medium are required to obtain cell quantities needed for their clinical use. In this proof of concept study, we cultivated human MSCs within a three-dimensional nanofibrillar cellulose (NFC) hydrogel. We show that NFC is biocompatible with human MSCs, and represents a feasible approach to upscaling of their culture.

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Acknowledgments

D.W. is supported by a grant of the DFG (German Research Foundation, WI4318/2-1). J.S. is supported by UPM Biochemicals, Helsinki, Finland. S.S.H. is supported by The Scientific and Technological Research Council of Turkey (TUBITAK/SBAG-112S587), Ankara, Turkey and Selcuk University Research Coordination Office (BAP), Konya, Turkey. We thank Professor Erdal Karaoz, Liv Hospital, Center for Regenerative Medicine and Stem Cell Research & Manufacturing (LivMedCell), Istanbul, Turkey for providing human bone marrow MSCs. The sponsors did not participate in study design and data analysis. We thank Amanpreet Kaur for the help with electron microscopy and Marie Zeuner for careful proof-reading.

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Authors and Affiliations

  1. Stem Cell Biology and Regenerative Medicine Group, School of Pharmacy, University of Reading, Whiteknights Campus, Reading, RG6 6AP, UK

    Ioannis Azoidis, Joel Metcalfe, Jonathan Sheard & Darius Widera

  2. Lein Applied Diagnostics Ltd, Reading Enterprise Centre, Whiteknights Road, Reading, RG6 6BU, UK

    James Reynolds

  3. Cell Migration Lab, School of Biological Sciences, University of Reading, Whiteknights Campus, Reading, RG6 6AP, UK

    Shirley Keeton

  4. Selcuk University Faculty of Dentistry Department of Periodontology Campus, 42079, Konya, Turkey

    Sema S. Hakki

  5. Sheard BioTech Limited, Suite LP36133, 20-22, Wenlock Road, London, N17GU, UK

    Jonathan Sheard

Authors
  1. Ioannis Azoidis
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  2. Joel Metcalfe
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  3. James Reynolds
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  4. Shirley Keeton
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  5. Sema S. Hakki
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  6. Jonathan Sheard
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  7. Darius Widera
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Correspondence to Darius Widera.

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Azoidis, I., Metcalfe, J., Reynolds, J. et al. Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels. MRS Communications 7, 458–465 (2017). https://doi.org/10.1557/mrc.2017.59

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  • DOI: https://doi.org/10.1557/mrc.2017.59

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