Abstract
Interactions between smooth muscle cells (SMCs) and biomaterials must not result in phenotype changes as this may generate uncontrolled multiplication processes and occlusions in vascular grafts. The aim of this study was to relate the hydrolytic stability and biocompatibility of polyurethanes (PUs) on SMCs. A higher polycaprolactone (PCL) concentration was found to improve the hydrolytic stability of the material and the adhesion of SMCs. A material with 5% polyethylene glycol, 90% PCL, and 5% pentaerythritol presented high cell viability and adhesion, suggesting a contractile phenotype in SMCs depending on the morphology. Nevertheless, all PUs retained their elastic modulus over 120 days, similar to the collagen of native arteries (~10 MPa). Furthermore, aortic SMCs did not present toxicity (viability over 80%) and demonstrated adherence without any abnormal cell multiplication processes, which is ideal for the function to be fulfiled in situ in the vascular grafts.
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Acknowledgments
The research and publication were supported by the Universidad de La Sabana (ING-205-2018) and the Minister of Science, Technology, and Innovation of the Republic of Colombia, MINCIENCAS (Contract number 80740-186-2019). M. M-G. would like to thank the Universidad de La Sabana for the scholarship for her master's studies. S. A-A. would like to thank MINCIENCIAS for the doctoral training scholarship (Grant 727-2015). The authors are thankful to Professor Ericsson Coy Barrera and his staff at Nueva Granada Military University for the access to the Varioskan™ LUX multimode microplate reader. J. A. S. acknowledges the financial support by MINECO through FIS2017-83295-P, MAT2015-71070-REDC, MAT2016-75586-C4-1/2/3-P and the Ramon y Cajal Fellowship (RYC-2015-17482). CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008–2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund.
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Morales-Gonzalez, M., Arévalo-Alquichire, S., Diaz, L.E. et al. Hydrolytic stability and biocompatibility on smooth muscle cells of polyethylene glycol–polycaprolactone-based polyurethanes. Journal of Materials Research 35, 3276–3285 (2020). https://doi.org/10.1557/jmr.2020.303
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DOI: https://doi.org/10.1557/jmr.2020.303