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In vitro evaluation of a novel multiwalled carbon nanotube/nanohydroxyapatite/polycaprolactone composite for bone tissue engineering

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Abstract

In this study, a three-phased multiwalled scaffold, composed of carbon nanotube (mwCNT), nanocrystalline hydroxyapatite (nHA), and polycaprolactone (PCL), was fabricated by the solvent evaporation technique. The structure character, mechanical properties, and degradation activity in simulated body fluid (SBF), along with osteoproductive ability in human osteosarcoma cell MG63, were investigated thoroughly. Results showed that the three phases in mwCNT/nHA/PCL composite presented excellent miscibility and stronger interfacial force when the weight content was 1/15/84 (wt%). Simultaneously, the composite had smaller porosity and slower degradation rate, and there was massive crystallized hydroxyapatite formed on the surface after being soaked in SBF. With regard to bioactivity, MG63s on this scaffolds presented good proliferation performance and differentiated into the osteogenic lineage by expressing high levels of ALP. It was concluded that mwCNTs/nHA/PCL composite scaffolds might be beneficial for bone tissue engineering at a relatively low concentration of mwCNTs and nHA.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatological Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510140, People’s Republic of China

    Huixiao Yang, Huishan Chen, Yuting Zhu, Meijuan Cai & Huling Lv

  2. Department of Breast Surgery, Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin, 300100, People’s Republic of China

    Jieqing Li

  3. Division of Cancer Research and Training, Department of Internal Medicine, Charles R. Drew University of Medicine and Science, Los Angeles, California, 90059, USA

    Jieqing Li

  4. Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education (Sichuan University) China, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, 610041, People’s Republic of China

    Qiong Liao

  5. Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine, Temple University, Philadelphia, Pennsylvania, 19140, USA

    Hua Guo

  6. State Key Laboratory of Oral Diseases, Sichuan University West China College of Stomatology, Sichuan University, Chengdu, Sichuan, 610041, People’s Republic of China

    Huling Lv

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  1. Huixiao Yang
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Yang, H., Li, J., Liao, Q. et al. In vitro evaluation of a novel multiwalled carbon nanotube/nanohydroxyapatite/polycaprolactone composite for bone tissue engineering. Journal of Materials Research 34, 532–544 (2019). https://doi.org/10.1557/jmr.2018.484

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